Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells

Yazawa, Ryosuke; Kubokawa, Tsubasa; Ichida, Kensuke; Kawamura, Wataru; Tani, Reoto; Kamio, Shigeharu; Morita, Tetsuro; Yoshizaki, Goro

doi:10.1007/s12562-020-01486-2

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…So far, only a few countries have achieved the reproduction of captive or reared bluefin tunas, despite their high demands and values [ 42 , 44 ], implying the difficulties in seed production of these species. To overcome these difficulties, xenotransplantation has been attempted with recipient species with advantages of a relatively smaller body, tolerance to environmental changes, shorter generation time, and well-established reproduction methodology, including blue drum [ 45 ], chub mackerel ( Scomber japonicus ) [ 22 ], Eastern little tuna ( Euthynnus affinis ) [ 46 ], yellowtail kingfish ( Seriola lalandi ) [ 47 ], or hybrid mackerel [ 48 ]. Although the successful production of gametes derived from the donor germ cells has not been reported, the incorporation of transplanted germ cells into recipients’ gonads was noted in these studies, indicating that these recipients could support the survival and migration of germ cells from bluefin tunas.…”

Section: Advantages Of Germ Cell Transplantation In Aquaculturementioning

confidence: 99%

“…152) Unsort: 16.6 Sort: 77.3 N. mitsukurii WT 12 dph PDGC Sort (PKH26 labled): 33.3 PDGC Sort (No. 152 AB labeled): 63.3 at 14 dpt N/A N/A N/A N/A N/A [ 45 ] WT 3 y >10,000 TCs PDGC N/A Hybrid ♂ S. japonicus × ♀ S. australasicus 10 d 100 at 14 dpt N/A N/A N/A N/A N/A [ 48 ] WT 2 y >30,000 TCs N/C N/A S. japonicus WT 7 dph Y at 14 dpt and 4 mpt N/A N/A N/A N/A N/A [ 46 ] E. affinis WT 10 dph Y at 14 dpt and 3 mpt …”

Section: Table A1mentioning

confidence: 99%

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Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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Germ cell transplantation technology enables surrogate offspring production in fish. This technology has been expected to mitigate reproductive barriers, such as long generation time, limited fecundity, and complex broodstock management, enhancing seed production and productivity in aquaculture. Many studies of germ cell transplantation in various fish species have been reported over a few decades. So far, surrogate offspring production has been achieved in many commercial species. In addition, the knowledge of fish germ cell biology and the related technologies that can enhance transplantation efficiency and productivity has been developed. Nevertheless, the commercial application of this technology still seems to lag behind, indicating that the established models are neither beneficial nor cost-effective enough to attract potential commercial users of this technology. Furthermore, there are existing bottlenecks in practical aspects such as impractical shortening of generation time, shortage of donor cells with limited resources, low efficiency, and unsuccessful surrogate offspring production in some fish species. These obstacles need to be overcome through further technology developments. Thus, we thoroughly reviewed the studies on fish germ cell transplantation reported to date, focusing on the practicality, and proposed potential solutions and future perspectives.

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Section: Advantages Of Germ Cell Transplantation In Aquaculturementioning

confidence: 99%

Section: Table A1mentioning

confidence: 99%

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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“…Gonads were fixed with Bouin's fixative overnight at 4°C, cut into 4μm thick sections using standard paraffin-embedding methods, and stained with hematoxylin and eosin. Freshly isolated testes were minced and incubated with 0.4% collagenase H (Roche Diagnostics) and 0.03% dispase II (Sanko Junyaku) in L-15 medium (pH 7.6; Life Technologies) containing 1% foetal bovine serum (Life Technologies) and 0.05% DNase I (Roche Diagnostics) at 25°C as previously described (Yazawa et al, 2010(Yazawa et al, , 2021. To remove spermatozoa and blood cells from whole testicular cell suspensions, density gradient centrifugation using a Percoll gradient (Percoll Plus; GE Healthcare) was performed as previously described (Ichida et al, 2019).…”

Section: Germ Cell Transplantationmentioning

confidence: 99%

“…At least 30,000 donor testicular cells were transplanted into the peritoneal cavity of hybrid mackerel larvae (total length, 5.8 ± 0.2 mm) at 10 days ph in April 2017. After transplantation, recipient larvae were transferred and raised in a 100-L seed production tank as previously described (Yazawa et al, 2010(Yazawa et al, , 2021.…”

Section: Germ Cell Transplantationmentioning

confidence: 99%

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Tani

Yazawa

Kamio

et al. 2022

Aquaculture Research

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In this study, we examined the feasibility of germ cell‐less hybrids, produced by a cross between female blue mackerel (Scomber australasicus) and male chub mackerel (S. japonicus), as recipients in order to develop a surrogate broodstock technology for mackerels, which is attracting attention as a new aquaculture target. Donor testicular cells, isolated from the testes of a chub mackerel, were transplanted into the peritoneal cavity of 500 hybrid mackerel larvae. Of the 42 male recipients that survived to 1 year of age, 35 matured and produced donor‐derived chub mackerel sperm. Amongst them, 22 produced only donor‐derived sperm, with none of the recipient's own sperm detected. Two of the six females that survived to 1‐year‐old produced donor‐derived eggs, and one of two matured females produced only donor‐derived eggs. Notably, when the mature recipients were housed in a single fish tank, some spontaneously mated, and normal hatchlings arising from fertilization of donor‐derived chub mackerel eggs and sperm were successfully obtained. Although the efficiency of this method needs to be improved in the future, hybrid mackerel can be used as surrogates for mackerel species breeding. This technology is expected to make a significant contribution to the breeding of mackerels.

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Reproductive Characteristics and Suitability of Sterile dead end Knockout Nibe Croaker as a Recipient for Intraperitoneal Germ Cell Transplantation

Yazawa,

Saitoh,

Yamauchi

et al. 2024

Mar Biotechnol

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The use of sterile recipients is crucial for e ciently producing donor-derived offspring through surrogate broodstock technology for practical aquaculture applications. Although knockout (KO) of the dead end (dnd) gene has been used in previous studies as a sterilization method, it has not been reported in marine sh. In this study, nibe croaker was utilized as a model for marine teleosts that produce small pelagic eggs, and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system was utilized to produce dnd KO sh. The F1 generation, which carried a nonsense mutation in the dnd gene, was produced by mating founder individuals with wild-type counterparts. Subsequently, the F2 generation was produced by mating the resulting males and females.Among the F2 generations, 24.0% consisted of homozygous KO individuals. Histological analysis revealed that primordial germ cells (PGCs) were present in homozygous KO individuals at 10 days post hatching (dph), similar to wildtype individuals. However, by 20 dph, PGCs were absent in KO individuals. Furthermore, no germ cells were observed in the gonads of both sexes of homozygous KO individuals at 6 months old, which is the typical maturity age for wild-type individuals of both sexes. In addition, when cryopreserved donor nibe croaker testicular cells were transplanted, only donor-derived offspring were successfully obtained through the spontaneous mating of homozygous KO recipient parents. Results indicate that dnd KO nibe croaker lack germ cells and can serve as promising recipients, producing only donor-derived gametes as surrogate broodstock.

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Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells

Cited by 7 publications

References 34 publications

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Reproductive Characteristics and Suitability of Sterile dead end Knockout Nibe Croaker as a Recipient for Intraperitoneal Germ Cell Transplantation

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