Establishment of a sensitive time-resolved fluoroimmunoassay for detection of Bacillus thuringiensis Cry1Ie toxin based nanobody from a phage display library

Xu, Chongxin; Liu, Xiaoqin; Zhang, Cunzheng; Zhang, Xiao; Zhong, Jianfeng; Liu, Yuan; Hu, Xiaodan; Lin, Manman

doi:10.1016/j.ab.2016.11.006

Cited by 20 publications

(7 citation statements)

References 30 publications

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“…Until now, mounting evidences about detection of various Cry toxins (Cry1Ac, Cry1Ie, Cry1C, Cry1Fa) base on Nbs have been published (Liu et al., ; Wang et al., ; Xu et al., ; Zhou et al., ). Among them, a series of papers about Cry1Ac, Cry1C, and Cry1Fa came from our laboratory.…”

Section: Resultsmentioning

confidence: 99%

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Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single‐domain antibodies

Zhong

et al. 2018

MicrobiologyOpen

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Bt Cry1B toxin, a residue in insect‐resistant transgenic plants, has been identified to be harmful to human health. Therefore, it is urgent to detect the Cry1B toxin level in each kind of transgenic plant. Nbs, with prominently unique physiochemical properties, are becoming more and more promising tools in the detection of target antigens. In this study, an immune phage display library that was of high quality was successfully constructed for the screening of Cry1B‐specific Nbs with excellent specificity, affinity, and thermostable. Subsequently, a novel sandwich ELISA for Cry1B detection was established, which was based on the biotin–streptavidin system using these aforementioned Nbs. This established detection system presented a linear working range from 5 to 1000 ng ml−1 and a low detection limit of 3.46 ng ml−1. The recoveries from spiked samples were in the range of 82.51%–113.56% with a relative standard deviation (RSD) lower than 5.00%. Taken together, the proposed sandwich ELISA would be a potential method for the detection of Cry1B toxin in transgenic Bt plants specifically and sensitively.

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Section: Resultsmentioning

confidence: 99%

“…Until now, mounting evidences about detection of various Cry toxins (Cry1Ac, Cry1Ie, Cry1C, Cry1Fa) base on Nbs have been published (Liu et al, 2016;Wang et al, 2014;Xu et al, 2017;Zhou et al, 2016).…”

Section: Recoveries Studymentioning

confidence: 99%

Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single‐domain antibodies

Zhong

et al. 2018

MicrobiologyOpen

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“…Por otro lado, la efectividad que ejercen los Nac al aplicarlos se extiende hasta la industria agrícola y de los alimentos, donde se han desarrollado como herramientas de identificación y detección de las toxinas producidas por microorganismos como las bacterias y los hongos principalmente. Por ejemplo, las micotoxinas de Aspergillus flavus (Ren et al, 2020), y la producida por Bacillus thuringiensis, que se han utilizado como un bio-insecticida efectivo para el control de plagas agrícolas (Xu et al, 2017). Para el año 2022, en Estados Unidos se espera el lanzamiento del primer biofungicida con el nombre "Biofun-1", el cual combina el reconocimiento de los Nac y antifúngicos contra Botrytis cinerea (Njeru & Kusolwa, 2021).…”

Section: Nanoanticuerpos Para La Industriaunclassified

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

et al. 2021

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Los anticuerpos monoclonales son una de las herramientas más revolucionarias en el área de la biomedicina por tener aplicaciones inmunoterapéuticas e inmunodiagnósticas. Sin embargo, el rápido avance tecnológico que demandan estas áreas genera la exploración de nuevas biomoléculas. El descubrimiento de anticuerpos compuestos únicamente por cadenas pesadas, presentes de forma natural en el suero de los camélidos y en algunas especies de tiburón, son motivo de estudio desde las últimas décadas como una alternativa a los anticuerpos convencionales. Estos poseen una región de reconocimiento antigénico, que consiste en un dominio variable por cada cadena, conocidos como anticuerpos de un solo dominio o nanoanticuerpos. Estas biomoléculas se caracterizan por tener un tamaño reducido, alta especificidad, estabilidad y bajo costo en su producción; propiedades que las convierten en una herramienta altamente versátil. En la presente revisión se abordarán aspectos relevantes de los nanoanticuerpos, como su descubrimiento, características estructurales, desarrollo en el campo de la biotecnología y su potencial de aplicación en enfermedades como el cáncer y en la identificación de microorganismos.

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“…At present, it is an effective means by using special chemiluminescent group labeled-antibody to improve the detection limit of antibody for antigen, and it has been widely used in immunology detection field (Acharya et al, 2016;Li et al, 2017;Sheng et al, 2016;You, Li, Li, & Ma, 2016). Eu 3+ is a kind of fluorescing rare earth metals ion with superiority of high luminous efficiency, long relaxation time and resist high background absorption, and it was widely labelled to antibody and then used to establish time-resolved fluoroimmunoassay for ultrasensitive determination of hazards in food and feed (Hu et al, 2018b;Xu et al, 2017;Zhang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

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Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu 3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive timeresolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC 50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong crossreactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples. Highlights (1) Prepared high-quality Van-KLH/BSA conjugates. (2) Obtained high activity and purify anti-Van polyclonal antibodies. (3) Prepared high-quality Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (4) Firstly established an IC-TRFIA for Van based on the anti-Van polyclonal antibodies and Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (5) The IC-TRFIA can be used for ultrasensitive determination of Van in milk powder samples.

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Establishment of a sensitive time-resolved fluoroimmunoassay for detection of Bacillus thuringiensis Cry1Ie toxin based nanobody from a phage display library

Cited by 20 publications

References 30 publications

Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single‐domain antibodies

Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single‐domain antibodies

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

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