Establishment of a Rat Model of Liver Venous Deprivation: Simultaneous Portal and Hepatic Vein Ligation

Zhang, Yuefeng; He, Xiaoqin; Ma, Peng; Xiong, Liangkun; Bai, Wenhui; Zhang, Gaoshuo; Xu, Yangtao; Song, Wei; Yu, Kaihuan

doi:10.14218/jcth.2022.00032

Cited by 5 publications

(3 citation statements)

References 29 publications

(50 reference statements)

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“…Each individual liver lobe was weighed and recorded. Because each rat liver lobe compared to the body weight in normal healthy SD rats is constant, our previous study showed that the FLR (SCL+ part of PP)/body weight is approximately 0.00248585; thus, the FLR growth ratio was calculated using the following formula [ 12 ]: …”

Section: Methodsmentioning

confidence: 99%

Extreme hepatectomy with modified ALPPS in a rat model: gradual portal vein restriction associated with hepatic artery restriction

He,

Zhang,

et al. 2023

BMC Surg

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Background & aim Associating liver partition and portal vein ligation (PVL) for staged hepatectomy (ALPPS) is a creative strategy for enlarging the future liver remnant (FLR) and increasing the tumor resectability rate. However, the indications for ALPPS must have a certain limit when the FLR is too small. We aimed to establish a modified ALPPS model with more widen applicability in rats. Methods An extreme ALPPS model was established in rodents with only a 6.5% FLR. The portal vein (PV) was subjected to restriction to different degrees, then the portal vein pressure (PVP) was measured. Then, different modifications of ALPPS, including hepatic artery restriction (HAR), gradual portal vein restriction (GPVR), and GPVR-associated HAR (HAR+GPVR), were applied in the extreme ALPPS models. Results PVL or PVR provoked an immediate increase in the PVP. The PVP in the PVR -1.28 mm, PVR -0.81 mm, PVR -0.63 mm, and PVL groups was 11.05±1.57 cmH2O, 16.18±1.92 cmH2O, 20.66±1.99 cmH2O, and 24.10±3.33 cmH2O, respectively, and the corresponding 3-day survival rate was 100%, 90.09%, 36.33% and 0, respectively. Then, in the extreme ALPPS model, the growth ratio of the FLR in the control, HAR, GPVR, and HAR+GPVR groups was 0.43±0.21, 0.50±0.16, 4.80±0.86, and 7.40±2.56, and as a consequence, the corresponding 30-day survival rate was 9.09%, 15.38%, 84.61% and 92.90%, respectively. Conclusion ALPPS itself has a limit, and high PVP after PVL contributes to postoperative death in the extreme ALPPS model. Furthermore, a modified method for extreme ALPPS is proposed, i.e., GPVR+HAR in place of PVL, which significantly improves the survival rate of extreme hepatectomy in rat models.

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Section: Methodsmentioning

confidence: 99%

Extreme hepatectomy with modified ALPPS in a rat model: gradual portal vein restriction associated with hepatic artery restriction

He,

Zhang,

et al. 2023

BMC Surg

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“…The results suggest that S1P promotes the production of IL-6 by human LSEC and that this effect enhances the proliferation of human hepatocytes, mainly through the activation of the STAT3 signaling pathway in a paracrine manner [ 64 ]. Serum and liver S1P concentrations were significantly increased in the associated liver partitioning and portal vein ligature for staged hepatectomy (ALPPS) groups after surgery in vivo [ 36 ]. The levels of ceramide and sphingosine increased, accompanied by increased levels of neutral sphingomyelinase localized in liver nuclei [ 65 ].…”

Section: Sphingolipid Metabolic Pathway In Liver Regenerationmentioning

confidence: 99%

“…Serum and liver S1P concentrations were significantly increased in the associated liver partitioning and portal vein ligature for staged hepatectomy (ALPPS) groups after surgery in vivo [36]. The levels of ceramide and sphingosine increased, accompanied by increased levels of neutral sphingomyelinase localized in liver nuclei [65].…”

Section: Sphingolipid Metabolic Pathway In Liver Regenerationmentioning

confidence: 99%

Critical Roles of the Sphingolipid Metabolic Pathway in Liver Regeneration, Hepatocellular Carcinoma Progression and Therapy

Nojima,

Shimizu,

Murakami

et al. 2024

Cancers

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The sphingolipid metabolic pathway, an important signaling pathway, plays a crucial role in various physiological processes including cell proliferation, survival, apoptosis, and immune regulation. The liver has the unique ability to regenerate using bioactive lipid mediators involving multiple sphingolipids, including ceramide and sphingosine 1-phosphate (S1P). Dysregulation of the balance between sphingomyelin, ceramide, and S1P has been implicated in the regulation of liver regeneration and diseases, including liver fibrosis and hepatocellular carcinoma (HCC). Understanding and modulating this balance may have therapeutic implications for tumor proliferation, progression, and metastasis in HCC. For cancer therapy, several inhibitors and activators of sphingolipid signaling, including ABC294640, SKI-II, and FTY720, have been discussed. Here, we elucidate the critical roles of the sphingolipid pathway in the regulation of liver regeneration, fibrosis, and HCC. Regulation of sphingolipids and their corresponding enzymes may considerably influence new insights into therapies for various liver disorders and diseases.

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Extended Ligation of the Hepatic Vein May Yield a Similar Effect to Liver Venous Deprivation in a Rat Model

Zhang

et al. 2023

Journal of Investigative Surgery

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Establishment of a Rat Model of Liver Venous Deprivation: Simultaneous Portal and Hepatic Vein Ligation

Cited by 5 publications

References 29 publications

Extreme hepatectomy with modified ALPPS in a rat model: gradual portal vein restriction associated with hepatic artery restriction

Extreme hepatectomy with modified ALPPS in a rat model: gradual portal vein restriction associated with hepatic artery restriction

Critical Roles of the Sphingolipid Metabolic Pathway in Liver Regeneration, Hepatocellular Carcinoma Progression and Therapy

Extended Ligation of the Hepatic Vein May Yield a Similar Effect to Liver Venous Deprivation in a Rat Model

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