Establishment of a Rapid Breeding System for Bletilla striata

Abstract

Bletilla striata (Thunb. ex A. Murray) Rchb. f., a species of perennial herb of orchidaceae that has remarkable effects and high economic value, has been intensively studied by many scholars. Although this herb has many seeds, the germination rate is exceptionally low, which leads to decreased germplasm resources and increased market demand every year. To solve this problem, this study examined the aseptic germination system and the direct seeding technology system. On Murashige and Skoog (MS) medium, 2.0 mg/L 6-benzylaminopurine (6-BA) and 1.0 mg/L naphthylacetic acid (NAA) were added before seed germination, and 70 g/L banana juice and 0.5 mg/L NAA were added when rooting. Then, the seedlings were transplanted to a mixed substrate of humus, river sand, and bark (volume ratio of 3:1:1). The direct seeding system consists of substrate treatment, sowing, seedling raising, seedling growth, and transplanting. Turfy soil, Huangjiang residue, and river sand were selected as the substrate. The results revealed that the germination rate was increased to 91.8%, whereas the plantlet regeneration was increased to 82.0%. After 180 days of cultivation, the plants could be transplanted as finished seedlings. The establishment of B. striata seedling system provides a safe, rapid, reliable production technology route for industrial development.

Conservation Horticulture: In Vitro Micropropagation and Acclimatization of Selected Florida Native Orchids

“…Auxins aid in the process of cell growth and expansion (Vendrame and Khoddamzadeh, 2016), whereas another important PGR widely used is BAP, which is responsible for cell division. There have been many studies showing the importance of growth regulators in the in vitro micropropagation of orchids, such as Bautista-Aguilar et al (2021) in Vanilla planifolia, Do et al (2019) in Paphiopedilum hybrids, Huang and Fang (2021) in Cymbidium goeringii, Mi et al (2021) in Bletillastriata, Chookoh et al (2019) in Tolumnia, andDe Stefano et al (2022) in Epidendrum nocturnum. However, a fast protocol has yet to be developed for the species used in this project.…”