“…Inclusion bodies were extracted as previously described [ 30 ]. Briefly, the biomass was resuspended in PBS (137 mM NaCl (Sigma-Aldrich, Saint Louis, MO, USA), 2.7 mM KCl (Sigma-Aldrich, Saint Louis, MO, USA), 10 mM Na 2 HPO 4 (Sigma-Aldrich, Saint Louis, MO, USA), and 1.8 mM KH 2 PO 4 (Merck, Darmstadt, Germany), pH 7.4) containing 0.1% Triton X-100 (Sigma-Aldrich, Saint Louis, MO, USA) and then lysed by mechanical cell disruption in the bead mill Dyno-mill ML (Wab, Muttenz/Basel, Switzerland).…”