Establishment of a primary culture of polymorphous low grade adenocarcinoma cells

Teixeira, Lucas Novaes; Montalli, Victor Ângelo Martins; Oliveira, Stanley Robson de Medeiros; Toledo, Thais Fernanda Santos; Martinez, Elizabeth Ferreira; Araújo, Vera Cavalcanti de

doi:10.1016/j.archoralbio.2017.06.015

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…Histological growth patterns of PAC and adenoid cystic carcinoma (ACC) have several overlaps, such as infiltrating tubular, cribriform and solid histology, presence of cystic spaces and neurotropism [12,17]. However, immunohistochemical staining pattern of PAC is diffusely positive for pan-CK including CK 7, vimentin and S100 protein [9,11,[18][19][20][21], but ACC is a biphasic tumor [22,23]. Myoepithelial markers (α-SMA and p40) are negative in PAC [18,20,24], but positive in ACC [22,24].…”

Section: Discussionmentioning

confidence: 99%

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Palatal Polymorphous Adenocarcinoma with High-Grade Transformation: A Case Report and Literature Review

Kikuchi

Nagao

Ide

et al. 2018

Head and Neck Pathol

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Polymorphous adenocarcinoma (PAC) is the second most common intraoral malignant neoplasm of the minor salivary glands. However, it is very rare for PAC to show high-grade transformation (HGT) and to our knowledge, the English literature only seven reported cases. HGT tends to be observed when PAC recurs, and it is extremely rare to be seen at initial presentation. Here we report a 43-year-old Japanese male patient with PAC of the right palate showing HGT at initial presentation. Histopathologically, the tumor was characterized by a prominent solid and papillary-cystic growth pattern, with nuclear atypia and necrosis in area of HGT. The immunohistochemical staining pattern was consistent with PAC, as the tumor cells showed diffuse positivity for cytokeratin, vimentin and S-100, and focal positivity for bcl-2, ɑ-SMA and EMA. The tumor cells in HGT areas were markedly positive for AR and Ki-67 (about 40%/HPF), and also focally positive for cyclin D1 and p53, whereas HER2/neu, ER, PgR, p63, D2-40, GCDFP-15, and mitochondria were negative. Here we present a very rare case of palatal PAC with HGT at initial presentation.

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Section: Discussionmentioning

confidence: 99%

“…Myoepithelial markers (α-SMA and p40) are negative in PAC [18,20,24], but positive in ACC [22,24]. Additionally, PRKD1 E710D mutations are largely restricted to classic PAC [21,[25][26][27]. Although PAC and ACC exhibit nearly similar histological patterns, their biologic behavior is significantly different [12,17].…”

Section: Discussionmentioning

confidence: 99%

Palatal Polymorphous Adenocarcinoma with High-Grade Transformation: A Case Report and Literature Review

Kikuchi

Nagao

Ide

et al. 2018

Head and Neck Pathol

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“…29,38 Indeed, the cell culture of PAC reveals 1 cell labeled by these 3 proteins. 39 Contrary to the p63 positivity detected in PAC, which could indicate an aberrant expression of the TAp63 isoform, 25 the absence of this protein in SC is not fully understood and has also been described previously. 29,[40][41][42][43] Concerning EMC, this neoplasm is composed of the dual cells of intercalated duct, both cell lines in a mature condition, which is proven by the cell culture of this tumor that showed, at least in the beginning, the presence of these 2 cells.…”

Section: Discussionmentioning

confidence: 88%

Comparison of p63/p40 Expression With Myoepithelial Markers in Minor Salivary Gland Tumors

et al. 2018

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The present study aimed to compare the expression of p63/p40 with smooth muscle actin (SMA) and vimentin (VIM) by myoepithelial cells in minor salivary gland tumors. Fifty-two formalin-fixed paraffin-embedded samples of minor salivary gland tumors derived from intercalated duct (pleomorphic adenoma [PA], adenoid cystic carcinoma [ACC], epithelialmyoepithelial carcinoma [EMC], polymorphous adenocarcinoma [PAC], and secretory carcinoma [SC]) and 3 samples of minor salivary gland tumors derived from excretory duct (mucoepidermoid carcinoma [MEC]) were evaluated by means of immunohistochemistry. The data were analyzed qualitatively. The results indicated that p63 and p40 expression were detected in myoepithelial cells present in PA, ACC, and EMC. However, both proteins were also observed in squamous areas of PA and all cases of MEC. SMA were noticed in some myoepithelial cells of PA, ACC, and EMC. Expression of SMA was negative in the other salivary gland tumors evaluated. VIM was constantly expressed by myoepithelial cells in PA, ACC, and EMC. VIM was also observed in cells of PAC and SC, but not in squamous areas of PA and MEC. In conclusion, p63 expression is almost comparable with VIM in detecting myoepithelial cells, an immunolabeling pattern not followed by p40, and consequently, caution has to be taken during the interpretation of salivary gland tumor exhibiting an p63/p40 phenotype in order to avoid a misdiagnosis.

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“…The primary cell culture of PAC was established using the explant method followed by density gradient centrifugation in Percoll in order to separate epithelial cells from mesenchymal cells [32]. The neoplastic epithelial cells were then seeded onto 25 cm 2 culture flasks (Corning Incorporated, Costar, Corning, New York, NY) containing DMEM (Nutricell, Campinas, SP, Brazil) supplemented with 10% (v/v) fetal bovine serum (Cultilab, Campinas, SP, Brazil), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma Aldrich, St. Louis, MO).…”

Section: Primary Cell Culture Derived From Polymorphous Adenocarcinomamentioning

confidence: 99%

Nrf2-peroxiredoxin I axis in polymorphous adenocarcinoma is associated with low matrix metalloproteinase 2 level

et al. 2017

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Polymorphous adenocarcinoma (PAC) is a malignant epithelial neoplasm that affects almost exclusively the minor salivary glands, generally described as having a relatively good prognosis. Aberrant nuclear factor erythroid 2 (NF-E2)-related factor (Nrf2) activation in tumor cells has been associated with induction of antioxidant enzymes, such as peroxiredoxin I (Prx I) and increased matrix metalloproteinase (MMP) expression. In this context, the aim of the present study was to evaluate the expression of Nrf2 and correlate it with Prx I and MMP-2 secretion in PAC. Thirty-one cases of PAC from oral biopsies were selected and immunohistochemically analyzed for Nrf2 and Prx I. MMP-2 quantification was performed on primary cell cultures derived from PAC. Oral squamous cell carcinoma (OSCC) cell cultures were used as control. A high immunoexpression of Nrf2 was observed in both the cytoplasm and the nucleus of neoplastic cells from PAC. Nuclear staining for Nrf2 suggested its activation in the majority of the PAC cells, which was confirmed by the high expression of its target gene, Prx I. Quantification of MMP-2 secretion showed lower levels in PAC cell cultures when compared to OSCC cell cultures (p < 0.05). In conclusion, although Nrf2 overexpression has been frequently associated with high-grade malignancies, such relationship is not infallible and, in fact, the opposite may occur in low-grade tumors, such as PAC of minor salivary glands.

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Establishment of a primary culture of polymorphous low grade adenocarcinoma cells

Cited by 5 publications

References 39 publications

Palatal Polymorphous Adenocarcinoma with High-Grade Transformation: A Case Report and Literature Review

Palatal Polymorphous Adenocarcinoma with High-Grade Transformation: A Case Report and Literature Review

Comparison of p63/p40 Expression With Myoepithelial Markers in Minor Salivary Gland Tumors

Nrf2-peroxiredoxin I axis in polymorphous adenocarcinoma is associated with low matrix metalloproteinase 2 level

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