Establishment of a Novel Bladder Cancer Xenograft Model in Humanized Immunodeficient Mice

Gong, Zhen; Xu, Hanzi; Su, Yiping; Wu, Wangfei; Hao, Lin; Han, Conghui

doi:10.1159/000430401

Cited by 16 publications

(17 citation statements)

References 36 publications

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“…It is well-known that CD4 + and CD8 + T lymphocytes are the primary helper and cytotoxic T cells, respectively, which can secrete cytokines and induce the death of tumor cells [31,32]. Furthermore, NK cells are an important population of lymphocytes and have pivotal roles in both innate and adaptive immunity [33].…”

Section: Discussionmentioning

confidence: 99%

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Tang

Huang

Xiong

et al. 2016

Cell Physiol Biochem

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Background: The previous studies have demonstrated that the polysaccharide isolated from Tarphochlamys affinis (PTA) exhibits anti-tumor effect on S₁₈₀ tumor-bearing mice and protective effects against hepatic injury. Methods: In this study, we investigated the anti-tumor activity and possible underlying mechanism of PTA on liver cancer using a murine H22 hepatocarcinoma model. Results: PTA was capable of repressing transplanted H22 solid hepatic tumor cell growth in vivo. The relative weight of immune organs (spleen and thymus) and lymphocyte proliferation induced by ConA or LPS were improved after PTA treatment. Furthermore, treatment with PTA promoted immune-stimulating serum cytokine secretion in H22 tumor-bearing mice. Additionally, the percentage of CD4⁺ T lymphocytes, CD8⁺ T lymphocytes and NK cells was increased in tumor-bearing mice following PTA administration. In tumor tissue, PTA significantly up-regulated the expression of Bax and p53 proteins and down-regulated the expression of Bcl-2 protein. In addition, at the therapeutic dose, PTA displayed very few toxic effects to major organs, such as the liver and kidney, in tumor-bearing mice. Conclusion: In H22 tumor-bearing mice, PTA exhibited prominent anti-tumor activity in vivo. The possible mechanism of action might be related to enhanced host immune system function and induction of H22 tumor cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Tang

Huang

Xiong

et al. 2016

Cell Physiol Biochem

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“…A number of animal models have been developed and used in basic tumor research. Due to the technical and ethical considerations, investigations into the immunobiology of allotransplantation can only be performed using animals, which are limited when translating the results to humans (22). Progress has been made with the development of different chimeric animal models, which can serve important roles in studies associated with cancer, transplantation biology, infectious diseases, and autoimmunity (23).…”

Section: Discussionmentioning

confidence: 99%

“…Based on previous studies, mice were transplanted with PBMCs at a dose of 4x10 7 /mouse, which has been shown to have an increased success rate of immune reconstruction and a reduced risk of graft vs. host disease (16,20,24). Among the various transplantation methods, the present study engrafted human PBMCs into NOD/SCID mice via an intraperitoneal injection because the method is more readily manipulated (22). After 4 weeks of immunization with human PBMCs, human CD45 + T-lymphocytes were detected in mice.…”

Section: Discussionmentioning

confidence: 99%

Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model

et al. 2018

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The antitumor effect of the human umbilical vein endothelial cell (HUVEC) vaccine has been well documented; however, its anti‑angiogenic effects on human esophageal squamous cell carcinoma (ESCC) have yet to be studied. In the present study, a 'humanized' mouse model was established by transplanting NOD/SCID mice with human peripheral blood mononuclear cells (PBMC). After 4 weeks, the level of cluster of differentiation (CD)‑45+ human T‑lymphocytes in mouse peripheral blood was >0.1%, which indicated that mouse reconstruction and the human immune system transformation had been successful. The humanized mice were used to evaluate the anti‑angiogenic effect of the HUVEC vaccine on human ESCC. After immunization with the HUVEC vaccine for 5 consecutive weeks, the humanized mice were subcutaneously transplanted with EC9706 cells. The results indicated that the HUVEC vaccine reduced the size of human esophageal carcinoma xenografts by suppressing angiogenesis. In addition, the HUVEC‑immunized mice exhibited reduced expression of angiogenesis‑associated antigens (vascular endothelial growth factor receptor 2 and VE‑Cadherin) in the tumor specimens, and increased levels of angiogenesis‑associated antibodies in the serum. Notably, the HUVEC vaccine also increased the infiltration of human T‑lymphocytes into the spleen of humanized mice. In conclusion, the present study demonstrated the anti‑angiogenic effect of the HUVEC vaccine on ESCC in a humanized mouse model, and set an experimental foundation for the application of the HUVEC vaccine in ESCC patients.

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“…Nude mice, purchased from Beijing laboratory animal center, were exposed to 2.5 Gy X-ray at a dose rate of 1.2 Gy/min (RS2000Pro, Rad Source Technologies, USA) [ 42 – 44 ]. On withdrawal of X-ray exposure after 2 days, the mice were randomly divided into three groups, LY-10 cells (1×10 7 cells per animal were injected subcutaneously into the right abdomen of mice from all four groups).…”

Section: Methodsmentioning

confidence: 99%

Sirt1 gene confers Adriamycin resistance in DLBCL via activating the PCG-1α mitochondrial metabolic pathway

Zhou

et al. 2020

Aging

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Sirt1 is closely related to cells aging, and Sirt1 also plays an important role in diffuse large B-cell lymphoma (DLBCL). However, its mechanism remains unclear. Therefore, we investigated the mechanism of Sirt1 mediated drug-resistance in DLBCL, while the recombinant lentivirus was used to regulate Sirt1 gene expression in DLBCL cell lines. Subsequently, the effect of Sirt1 on DLBCL resistance to Adriamycin was analyzed in vitro. The results show that Sirt1 overexpression confers Adriamycin resistance in DLBCL cell lines. However, inhibition of Sirt1 sensitized DLBCL cell lines to Adriamycin cytotoxicity. Additionally, tumor-bearing mice were used to verify that Sirt1 overexpression confers Adriamycin resistance in vivo after chemotherapy. In addition, we used secondgeneration sequencing technology and bioinformatics analysis to find that Sirt1 mediated drug-resistance is related to the Peroxisome proliferator-activated receptor (PPAR) signaling pathway, especially to PGC-1α. Interestingly, the mitochondrial energy inhibitor, tigecycline, combined with Adriamycin reversed the cellular resistance caused by Sirt1 overexpression in vivo. Moreover, western blotting and CO-IP assay reconfirmed that Sirt1-mediated drug-resistance is associated with the increased expression of PGC1-α, which induce mitochondrial biogenesis. In summary, this study confirms that Sirt1 is a potential target for DLBCL treatment.

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Establishment of a Novel Bladder Cancer Xenograft Model in Humanized Immunodeficient Mice

Cited by 16 publications

References 36 publications

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice

Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model

Sirt1 gene confers Adriamycin resistance in DLBCL via activating the PCG-1α mitochondrial metabolic pathway

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