Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2: molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization

Abstract

BackgroundPleomorphic malignant fibrous histiocytoma (MFH) is one of the most frequent malignant soft tissue tumors in adults. Despite the considerable amount of research on MFH cell lines, their characterization at a molecular cytogenetic level has not been extensively analyzed.Methods and resultsWe established a new permanent human cell line, FU-MFH-2, from a metastatic pleomorphic MFH of a 72-year-old Japanese man, and applied multicolor fluorescence in situ hybridization (M-FISH), Urovysion™ FISH, and comparative genomic hybridization (CGH) for the characterization of chromosomal aberrations. FU-MFH-2 cells were spindle or polygonal in shape with oval nuclei, and were successfully maintained in vitro for over 80 passages. The histological features of heterotransplanted tumors in severe combined immunodeficiency mice were essentially the same as those of the original tumor. Cytogenetic and M-FISH analyses displayed a hypotriploid karyotype with numerous structural aberrations. Urovysion™ FISH revealed a homozygous deletion of the p16INK4A locus on chromosome band 9p21. CGH analysis showed a high-level amplification of 9q31-q34, gains of 1p12-p34.3, 2p21, 2q11.2-q21, 3p, 4p, 6q22-qter, 8p11.2, 8q11.2-q21.1, 9q21-qter, 11q13, 12q24, 15q21-qter, 16p13, 17, 20, and X, and losses of 1q43-qter, 4q32-qter, 5q14-q23, 7q32-qter, 8p21-pter, 8q23, 9p21-pter, 10p11.2-p13, and 10q11.2-q22.ConclusionThe FU-MFH-2 cell line will be a particularly useful model for studying molecular pathogenesis of human pleomorphic MFH.

Malignant fibrous histiocytoma: Changing perceptions and management challenges

“…[1][2][3][4][5] Several recent studies, however, have described unique molecular and morphologic features, alongside behavioral features, of MFH that are not found in AFX or other tumor types. [6][7][8][9] The goal of this review is to analyze the current medical and scientific literature regarding MFH from the perspective of the practicing clinician in order to provide practical guidance regarding diagnosis and management of these tumors.…”

An approach to pleomorphic sarcomas: can we subclassify, and does it matter?

“…27 More recently, a number of studies using comparative genomic hybridization have evaluated UPS and compared the findings to those of other pleomorphic sarcomas. [28][29][30][31] Interestingly, several studies have found striking similarities between UPS and pleomorphic leiomyosarcoma suggesting a shared lineage. 29,31 Clinical behavior…”

Characterization of a novel radiation‐induced sarcoma cell line

“…It would be preferable to compare UACC‐SARC1 to other RIS cell lines; unfortunately no other such lines are available to the scientific community. When comparing the RIS cell line UACC‐SARC1 to a metastatic MFH cell line that was not a RIS, both show numerous structural abnormalities on cytogenetic analysis . However, UACC‐SARC1 showed a relatively higher number of chromosomal losses versus gains.…”