Establishment of a Mutant Library of Fragaria nilgerrensis Schlechtendal ex J. Gay via EMS Mutagenesis

Jiang, Shu; Wang, Mingqian; Zhao, Chenglin; Cui, Yuchen; Cai, Zhi; Zhao, Jun; Zheng, Yang; Li, Xue; Li, Jiajun

doi:10.3390/horticulturae8111061

Cited by 2 publications

(2 citation statements)

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“…The morphological changes in leaves are a typical observation in mutant libraries. In both the M 1 and M 2 generations, the leaf-type variant mutants were the majority in our study (Tables 2 and 3), which is consistent with the findings in other species, such as cucumber [7], strawberry [12], and eggplant [11]. Among these leaf-type mutants, changes in color were the most common mutants.…”

Section: Discussionsupporting

confidence: 91%

“…EMS has been widely adopted as a practical mutagen and is highly preferred owing to its high mutagenicity and ease of handling. For example, it has been used to create mutants in Arabidopsis [8], tomato (Solanum lycopersicum L.) [9,10], eggplant (Solanum melongena L.) [11], and strawberry (Fragaria × ananassa) [12]. Moreover, there are also numerous studies on EMS-induced mutant libraries in Cucurbitaceae crops.…”

Section: Introductionmentioning

confidence: 99%

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Construction of Watermelon Mutant Library Based on 60Co γ-ray Irradiation and EMS Treatment for Germplasm Innovation

Yin,

Hou,

Chen

et al. 2023

Horticulturae

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Watermelon is a crucial horticultural crop worldwide but its genetic base has become extremely narrow owing to long-term cultivation. Induced mutagenesis can create a range of variations with distinctive agricultural characteristics. To broaden the genetic diversity of watermelon, we established a mutagenesis library containing over 4000 M1 seeds from an inbred line ‘M08’, which was irradiated by 350 Gy of 60Co γ-rays for 3 h. The rates of germination, emergence, and survival of the M1 seeds were reduced by 5.88%, 18.66%, and 41.96%, respectively. After phenotypic screening, 20 and 10 types of morphological changes were observed in the M1 and M2 generations, with approximately 10.57% and 14.17% mutation frequencies, respectively. Six mutants with desirable horticultural alterations were selected for additional presentation, including the leaf color mutant C1-NO.1, the yellow peel mutant C1-NO.2, the pericarp thickening mutant C1-NO.3, the pericarp thinning mutant C1-NO.4, the seedless mutant C1-NO.5, and the C2-No.1 mutant with normal female flowers and malformed male flowers. Moreover, the three mutants M1-3, M2-1, and M1-5 were identified from our EMS-induced M2 library, exhibiting the fusiform fruit, the dark green peel, and the yellow leaves, respectively. Compared to the wild type (WT), the photosynthetic pigments and parameters were negatively impacted in the yellow-leaf mutant M1-5. For example, the total chlorophyll was 1.22 and 2.22 mg/g in the young and mature leaves of M1-5, respectively, which were significantly lower than those in the WT (2.58 and 2.90 mg/g, respectively). Notably, some mutagenesis phenotypes could be stably inherited, including traits such as yellow leaf color, fusiform fruit shape, and thickening and thinning pericarp. Taken together, these results indicate that these two mutant libraries serve as essential resources to discover new phenotypic germplasms, thereby facilitating the genetic breeding and functional gene exploration in watermelon.

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Section: Discussionsupporting

confidence: 91%