Establishment of a Large Animal Model for Eustachian Tube Functional Study in Miniature Pigs

An, Fengwei; Hu, Yuan; Guo, Weiwei; Hou, Zeng-Guang; Cai, Jianming; Luo, Chuncai; Yu, Ning; Jiang, Qingqing; Cheng, Wei; Liu, Wei; Yang, Shiming

doi:10.1002/ar.24098

Cited by 7 publications

(7 citation statements)

References 30 publications

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“… An et al. (2019) obtained qualitative and quantitative information on the eustachian tube (ET) by studying 16 domestic small experimental pigs and measuring their eustachian tube structures.…”

Section: Resultsmentioning

confidence: 99%

Viewing the current situation of pig model application in China’s medical field from the application and funding of NSFC

Zhang

Yang

2021

Journal of Otology

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Background The National Natural Science Foundation of China (NSFC) is an important part of China’s innovation system. In the last decade, the pig has become more and more widely used in the field of medical science, especially in otology research. Objective By analyzing and summarizing the funding information over recent years, we intend to identify the characteristics and trends of funding for research using pig models and provide references for future development. Material and methods This is a comprehensive analysis of features in funding for research projects involving pig models by the NSFC in the past 10 years, with a focus on projects in the field of otolaryngology/head and neck surgery. Results Both the number and amount of funding provided by the NSFC for research involving pig models are on the rise with each passing year. Researchers at the PLA General Hospital have completed a number of studies using miniature pigs in cochlear morphology, electrophysiology, cochlear implantation, cochlear transcription analysis, gene therapy, inner ear disease modeling and Eustachian tube pathology modeling. Conclusion Pigs as an ideal large mammal model are well suited in the current national basic research strategy in China, and can help further strengthen China’s leading position in basic research in the world.

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Section: Resultsmentioning

confidence: 99%

Viewing the current situation of pig model application in China’s medical field from the application and funding of NSFC

Zhang

Yang

2021

Journal of Otology

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“…Our study investigated the overall shape and dimensions of the porcine ET using the porcine ET phantom. A previous study reported the average total length of the ET as 24.2 mm using a cadaveric miniature porcine ET model 21 . This was close to the average length of the porcine ET phantom (23.18 mm) observed in our study.…”

Section: Discussionmentioning

confidence: 99%

Tapered self-expandable metallic stent optimized for Eustachian tube morphology in a porcine ET model

Kang

Kim

Ryu

et al. 2022

Sci Rep

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Several investigations on the feasibility of stent placement into the Eustachian tube (ET) are being conducted. However, stents optimized for the anatomical structure of the ET have not yet been developed. In this study, the efficacy and safety of a self-expandable metallic stent (SEMS) optimized for porcine ET morphology was investigated. Silicone was injected into a cadaveric porcine ET to analyze the ET morphology. The three-dimensional-reconstructed porcine ET phantom images obtained after a computed tomography scan were measured to determine the dimensions of the porcine ET. The SEMS was designed as a tapered structure on the basis of the morphological findings of the porcine ET. The tapered SEMS (T-SEMS) and conventional SEMS (C-SEMS) were placed into the porcine ET to compare the safety and efficacy of the two types of SEMSs. Stent-induced tissue hyperplasia in the T-SEMS group was significantly lower than that in the C-SEMS group (p < 0.001). The T-SEMS optimized for the porcine ET was effective in maintaining stent patency. T-SEMS seems to be better than C-SEMS in suppressing stent-induced tissue hyperplasia, owing to the reduced stent-mediated mechanical injuries and maintaining ET patency.

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“…The technical feasibility of stent placement in a porcine ET was assessed by fluoroscopic and endoscopic images obtained during the procedure and computed tomography (CT) afterward. The stent sizes were selected according to the diameter (2.3 to 3.4 mm) and length (24.2 to 36.4 mm) of the normal ET in a porcine model [15,16]. This study was approved by the Institutional Animal Care and Use Committee of the Asan Institute for Life Sciences (2020-12-189) and conformed to US National Institutes of Health guidelines for humane handling of laboratory animals.…”

Section: Methodsmentioning

confidence: 99%

Technical Feasibility and Histological Analysis of Balloon-Expandable Metallic Stent Placement in a Porcine Eustachian Tube

Kim

Kang

et al. 2021

Applied Sciences

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There is a clinical need to develop a stent to treat obstructive and refractory Eustachian tube dysfunction (ETD) after balloon Eustachian tuboplasty. An animal model for stent placement in the Eustachian tube (ET) is needed to develop optimal designs and materials, as stents for ETD have not been clinically applied. The purpose of this study was to evaluate the technical feasibility of stent placement and histological changes in a porcine ET model. Six ETs were evaluated in three pigs. Cobalt–chrome alloy stents with two different diameters were placed in the left and right ET of each animal (right, 3.5 mm; left, 2.5 mm). The outcomes were assessed by endoscopic and fluoroscopic imaging during the procedure, computed tomography after the procedure, and by histological examinations. Stent placement was technically successful in all specimens after metallic guiding sheaths were located in the nasopharyngeal end of the ET. The mean luminal diameters of the proximal, middle, and distal portions of the larger stents in the right ETs were 3.48 mm, 2.54 mm, and 2.15 mm, respectively. In the left ETs using smaller stents, these values were 2.49 mm, 1.73 mm, and 1.42 mm, respectively. The diameters of the inserted stents differed by stent location and the original diameter. Histological findings showed tissue hyperplasia with severe inflammatory cell infiltration at 4 weeks after stent placement. In conclusion, stent placement into the porcine ET was technically feasible, and stent-induced tissue hyperplasia was significantly evident. The luminal configuration of the placed ET stent changed according to its non-elastic nature and anatomical features of the porcine ET. Using this model, ET stents of various materials and designs with anti-inflammatory or anti-proliferative drugs can be optimized for future treatments of ET dysfunction.

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Establishment of a Large Animal Model for Eustachian Tube Functional Study in Miniature Pigs

Cited by 7 publications

References 30 publications

Viewing the current situation of pig model application in China’s medical field from the application and funding of NSFC

Viewing the current situation of pig model application in China’s medical field from the application and funding of NSFC

Tapered self-expandable metallic stent optimized for Eustachian tube morphology in a porcine ET model

Technical Feasibility and Histological Analysis of Balloon-Expandable Metallic Stent Placement in a Porcine Eustachian Tube

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