Establishment of a human hemangiosarcoma cell line (ISO-HAS)

Masuzawa, Mikio; Fujimura, Takao; Hamada, Yuhko; Fujita, Yuhsuke; Hara, Hisamichi; Nishiyama, Shigeo; Katsuoka, Kensei; Tamauchi, Hidekazu; Sakurai, Yoshinori

doi:10.1002/(sici)1097-0215(19990412)81:2<305::aid-ijc22>3.0.co;2-z

Cited by 79 publications

(76 citation statements)

References 18 publications

(31 reference statements)

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“…They were cultured as previously described, [34][35][36] and were used in fewer than 6 mo of continuous passage. The cell lines were incubated with or without 10 ng/mL or 100 ng/mL recombinant human IFNg (R&D) for 24 h before analysis.…”

Section: Cell Linementioning

confidence: 99%

Infiltration of PD-1-positive cells in combination with tumor site PD-L1 expression is a positive prognostic factor in cutaneous angiosarcoma

et al. 2016

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Cutaneous angiosarcoma (CAS) is a malignant sarcoma with poor prognosis. Programmed cell death-1 (PD-1)/programmed cell death-1 ligand-1 (PD-L1) expression reflects antitumor immunity, and is associated with patient prognosis in various cancers. The purpose of this study is to investigate the relationship between PD-1/PD-L1 expression and CAS prognosis. CAS cases (n D 106) were immunohistochemically studied for PD-L1 and PD-1 expression, and the correlation with patient prognosis was analyzed. PD-L1 expression was assessed by flow cytometry on three CAS cell lines with or without IFNg stimulation. A total of 30.2% of patients' samples were positive for PD-L1, and 17.9% showed a high infiltration of PD-1-positive cells. Univariate analysis showed a significant relationship between a high infiltration of PD-1-positive cells with tumor site PD-L1 expression and favorable survival in stage 1 patients (p D 0.014, log-rank test). Multivariable Cox-proportional hazard regression analysis also showed that patients with a high infiltration of PD-1-positive cells with tumor site PD-L1 expression were more likely to have favorable survival, after adjustment with possible confounders (hazard ratio (HR) D 0.38, p D 0.021, 95% confidence interval (CI) 0.16-0.86). Immunofluorescence staining of CAS samples revealed that PD-L1-positive cells were adjacent to PD-1-positive cells and/or tumor stroma with high IFNg expression. In vitro stimulation with IFNg increased PD-L1 expression in two out of three established CAS cell lines. Our results suggest that PD-1/PD-L1 expression is related to CAS progression, and the treatment with anti-PD-1 antibodies could be a new therapeutic option for CAS.

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Section: Cell Linementioning

confidence: 99%

Infiltration of PD-1-positive cells in combination with tumor site PD-L1 expression is a positive prognostic factor in cutaneous angiosarcoma

et al. 2016

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“…9,11,23 In part, the paucity of effective treatments for endothelial cancers is due to the lack of appropriate reagents to study these diseases. [24][25][26][27][28][29] Thus, our objective was to establish and characterize a cohort of canine HSA cell lines that could be used to explore the molecular and cellular features of this disease and that could serve as a resource to explore comparative aspects of canine and human endothelial tumors for therapeutic development.…”

mentioning

confidence: 99%

Canine malignant hemangiosarcoma as a model of primitive angiogenic endothelium

Fosmire

Dickerson

Scott

et al. 2004

Laboratory Investigation

123

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Hemangiosarcoma (HSA) is a common untreatable cancer of dogs that resembles human angiosarcoma. Detailed studies of these diseases have been historically hindered by the paucity of suitable reagents. Here, we show that expression of CD117 (c-Kit) can distinguish primitive (malignant) from mature (benign) proliferative endothelial lesions, and we describe eight independent cell lines derived from canine HSA explants. Endothelial origin was confirmed by sustained expression of surface CD105 (endoglin), CD146 (MUC18), and CD51/CD61 (a v b 3 integrin). The cell lines showed anchorage-independent growth and were motile and invasive when cultured on a basement membrane matrix. They required endothelial growth factors for growth and survival, and they could be induced to form tubular structures resembling blood vessels when cultured under low calcium conditions. The formation of vessel-like structures was blocked by nicotine, and restored by FK506, suggesting that 'nuclear factor of activated T cells' activity prevents differentiation of these cells. In summary, these cell lines represent a unique and novel resource to improve our understanding of endothelial cell biology in general and canine HSA in particular.

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“…Protocols 3 and 4 detailed below were based on methods described by Krump-Konvalinkova et al [154] and Masuzawa et al [156], yielding ASM and ISO-HAS respectively.…”

Section: Invasionmentioning

confidence: 99%

“…ASM and ISO-HAS have been identified as malignant endothelial cell lines [154,156,158]. Characterisation studies were performed to confirm that ASM and ISO-HAS cells …”

Section: Characterisation Of Angiosarcoma Cell Linesmentioning

confidence: 99%