Establishment of a human cell line (mono mac 6) with characteristics of mature monocytes

Abstract

A monocytic cell line, termed Mono Mac, was established from peripheral blood of a patient with monoblastic leukemia. Two clones, designated Mono Mac I and Mono Mac 6, were isolated and both were assigned to the monocyte lineage on the basis of morphological, cytochemical and immunological criteria. Most importantly, the clones express NaF-sensitive non-specific-esterase, produce reactive oxygen and stain with MAb My4. Mono Mac 6, in addition, constitutively exhibits phagocytosis of antibody-coated erythrocytes in 80% of the cells and reacts with a panel of MAbs that are specific for mature monocytes, i.e., M42, LeuM3, 63D3, Mo2 and UCHMI. By contrast, the monoblastic cell lines U937 and THP-I are negative for all these markers. Only expression of My4 could be detected after differentiation induced by interferon-gamma (IFN-gamma). Similar treatment of Mono Mac I, however, resulted in staining with all the monocyte-specific MAbs mentioned above, while IFN-gamma treatment of Mono Mac 6 enhanced antigen expression. In addition, the cells showed an increased frequency of multinucleated cells with a rise from 4.8% to 21.9%. Mono Mac 6 appears to be the only one of the cell lines studied to constitutively express phenotypic and functional features of mature monocytes.

Mechanisms of differential regulation of interleukin‐6 mRNA accumulation by tumor necrosis factor alpha and lymphotoxin during monocytic differentiation

“…HL 60, U 937 and THP-1 cells were purchased from the American Type Culture Collection, the MonoMac 1 line was kindly provided by H.W.L. Ziegler-Heitbrock, Institute for Immunology, University of Munich, FRG [15].…”

Multiplication and death-type of leukemia cell lines exposed to very long-chain polyunsaturated fatty acids

“…Sensitivity to PUFA was not associated with any particular cell lineage or clinical origin of the transformed cells. [24][25][26] The sensitivity to PUFA increased with increasing concentration of PUFA. All the untreated cultures multiplied with a similar doubling time of 23-28 h in our experiments.…”

Differential regulation of matrix metalloproteinase 2 and matrix metalloproteinase 9 by activated protein C: Relevance to inflammation in rheumatoid arthritis

“…Human monocytes and Mac6 cells, a cell line characteristic of mature monocytes (28), were maintained in RPMI 1640 containing 10% FCS and were supplemented with 2 mM L-glutamine, 100 units/ml of penicillin, and 100 g/ml of streptomycin. Before treatment, cells were preincubated for 2 hours in either DMEM or RPMI 1640 with 2% MMP-free FCS (basal medium) and then transferred to a fresh basal medium.…”