Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas

Lieber, Michael M.; Mazzetta, Joann; Nelson‐Rees, Walter A.; Kaplan, Michael; Todaro, George J.

doi:10.1002/ijc.2910150505

Cited by 427 publications

(267 citation statements)

References 15 publications

Supporting

Mentioning

261

Contrasting

Unclassified

Order By: Relevance

“…21 To investigate whether this bystander effect is dependent on direct cell-to-cell contact, as is the case for the HSVtk-mediated effect 15 or whether it is mediated by a freely diffusable metabolite, cells expressing CYP2B1 and nonexpressing reporter cells were separated by a filter with 0.45 m pores. To simulate the situation during treatment of a pancreatic tumour, a human pancreatic tumour-derived cell line, PANC1 17,22 was used. The combination of non-CYP2B1-expressing cells and PANC1 cells showed no significant change in cell number or viability when treated with pharmacologically active ifosfamide concentrations (Figure 1c).…”

Section: Resultsmentioning

confidence: 99%

Targeted chemotherapy by intratumour injection of encapsulated cells engineered to produce CYP2B1, an ifosfamide activating cytochrome P450

et al. 1998

View full text Add to dashboard Cite

The prognosis of pancreatic adenocarcinoma is poor and agent ifosfamide to toxic metabolites. Administration of current treatment ineffective. A novel treatment strategy is ifosfamide to tumour-bearing mice that were recipients of described here using a mouse model system for pancreatic implanted encapsulated cells results in partial or even comcancer. Cells that have been genetically modified to plete tumour ablation. These results suggest that in situ express the cytochrome P450 2B1 enzyme are encapsuchemotherapy with genetically modified cells in an lated in cellulose sulphate and implanted into pre-estabimmunoprotected environment may prove useful for lished tumours derived from human pancreatic cells. application in man. Cytochrome P450 2B1 converts the chemotherapeutic

show abstract

Section: Resultsmentioning

confidence: 99%

Targeted chemotherapy by intratumour injection of encapsulated cells engineered to produce CYP2B1, an ifosfamide activating cytochrome P450

et al. 1998

View full text Add to dashboard Cite

show abstract

“…In this study, eight of the 12 cell lines were found to produce CA19-9, and several produced CEA or DUPAN-2 as well. The four remaining cell lines were characterised mainly by morphological analysis (Lieber et al, 1975;Yunis et al, 1977;Owens et al, 1979;Yamada et al, 1986;Ikeda et al, 1990), which suggested that they originated from pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

Oikawa¹,

Kushuhara²,

Ishikawa³

et al. 1994

Br J Cancer

View full text Add to dashboard Cite

Summary Analysis of bioactive substances produced by cancer cells is one approach to understanding the biological features of human cancer. One of these bioactive substances is endothelin (ET)-1, a peptide with potent vasoconstrictive activity produced by vascular endothelial cells. We have previously reported the production of ET-1 by several types of human cancer, especially pancreatic cancer cells. To elucidate whether these cancer cells might share biological characteristics with vascular endothelial cells, we investigated the production of three ET isoforms in pancreatic cancer cells, using a specific radioimmunoassay. Further, we also investigated whether these cells produce thrombomodulin (TM), another product of endothelial cells functioning as a modulator of procoagulant activity. ET-1 was detected in 11 of 12 pancreatic cancer cell lines (92%) while ET-2 and ET-3 were detectable in only one cell line. Gel filtration analysis confirmed the presence of ET-1. Moreover, TM was detected in the cell lysates of 11 of the 12 cell lines (92%) and it was released into the culture medium in the majority (58%) of these cell lines. TM mRNA was also detected in these cells. In addition, TM was demonstrated immunocytochemically along the cell surface. These results suggest that pancreatic cancer cells share two characteristics with endothelial cells: the production of ET-1 and TM.

show abstract

“…The Panc1 cell line, a poorly differentiated (morphologically and phenotypically) pancreatic adenocarcinoma cell line (Lieber et al, 1975), was obtained from the American Type Culture Collection (ATCC). The well-differentiated pancreatic tumor cell line, CD11/HPAF, was established at Duke University (Kim et al, 1989).…”

Section: Cell Linesmentioning

confidence: 99%

The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis

et al. 2006

View full text Add to dashboard Cite

The 19q13 amplicon in pancreatic cancer cells contains a novel pancreatic differentiation 2 (PD2) gene (accession number AJ401156), which was identified by differential screening analysis. PD2 is the human homologue of the RNA polymerase II-associated factor 1 (hPaf1). In yeast, Paf1 is part of the transcription machinery, acting as a docking protein in between the complexes Rad6-Bre1, COMPASS-Dot1p, and the phosphorylated carboxyl terminal domain of the RNA polymerase II. As such, Paf1 is directly involved in transcription elongation via histone H2B ubiquitination and histone H3 methylation. The PD2 sequence is highly conserved from Drosophila to humans with up to 98% identity between rodent and human, suggesting the functional importance of PD2/ hPaf1 to maintain cellular homeostasis. PD2 is a modular protein composed of RNA recognition motif, DEADboxes, an aspartic/serine (DS)-domain, a regulator of the chromosome condensation domain and myc-type helixloop-helix domains. Our results further showed that PD2 is a nuclear 80 kDa protein, which interacts with RNA polymerase II. In addition, we have demonstrated that the overexpression of PD2 in the NIH 3T3 cells result in enhanced growth rates in vitro and tumor formation in vivo. Altogether, this paper presents strong evidence that the overexpression of PD2/hPaf1 is involved in cancer development.

show abstract

Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas

Cited by 427 publications

References 15 publications

Targeted chemotherapy by intratumour injection of encapsulated cells engineered to produce CYP2B1, an ifosfamide activating cytochrome P450

Targeted chemotherapy by intratumour injection of encapsulated cells engineered to produce CYP2B1, an ifosfamide activating cytochrome P450

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis

Contact Info

Product

Resources

About