Establishment of a comprehensive reference transcriptome for vertebral bone tissue to study the impacts of nutritional phosphorus deficiency in rainbow trout (Oncorhynchus mykiss, Walbaum)

Luyer, Jérémy Le; Deschamps, Marie‐Hélène; Proulx, Émilie; Stewart, N. Poirier; Beauparlant, Charles Joly; Droit, Arnaud; Robert, Claude; Vandenberg, Grant W.

doi:10.1016/j.margen.2014.10.002

Cited by 4 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was thus important to assess whether molecular activity or overall mineral status might be biased by the high cellular activity within the basalias. Both osteoblasts and chondrocytes differentiate from mesenchymal stem cells and thus share numerous key events involved in their differentiation and regulation [26]. Here, we observed that pathways involved in chondrocyte differentiation were affected in deformed fish (down-regulation of SHH and FGFR4) but not the expression level of a key mediator of cartilage formation SOX9 [98] and of MEF2C, a marker of chondrogenesis [23,99], which remained constant.…”

Section: Resultsmentioning

confidence: 74%

“…The mechanisms by which osteoblasts and osteoclasts control bone formation are not fully understood. However, various metabolic pathways affecting bone and cartilage formation have been identified in mammals and recent whole-transcriptome annotation provided evidence that these pathways were also present in teleosts, which suggests high conservation during osteichthyan evolution [26,27]. Genome-wide experiments revealed that most of the pathways (e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, a large-scale study on the impact of photoperiod on vertebral bone formation was conducted in Atlantic salmon [25] and highlighted the structural role of COL11a1 in the occurrence of vertebral deformities. Recently, characterization of vertebrae transcriptomes in sea bream (Sparus auratus) [26] and rainbow trout [27] suggested that homologs of key genes involved in bone metabolism are conserved in vertebrates. As bone metabolism in fish is complex, transcriptomic-wide analyses may be the best approach to identify the principal affected pathways during P deficiency.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

J¹,

MH²

2015

Next Generat Sequenc & Applic

View full text Add to dashboard Cite

International audienceThe prevalence of bone deformities, particularly linked with mineral deficiency, is an important issue for fish production. Juvenile triploid rainbow trout (Oncorhynchus mykiss) were fed a low-phosphorus (P) diet for 27 weeks (60 to 630 g body mass). At study termination, 24.9% of the fish fed the low-P diet displayed homogeneous biconcave vertebrae (deformed vertebrae phenotype), while 5.5% displayed normal vertebral phenotypes for the entire experiment. The aim of our study was to characterize the deformed phenotype and identify the putative genes involved in the appearance of P deficiency-induced deformities. Both P status and biomechanical measurements showed that deformed vertebrae were significantly less mineralized (55.0 ± 0.4 and 59.4 ± 0.5,% ash DM, for deformed and normal vertebrae, respectively) resulting in a lower stiffness (80.3 ± 9.0 and 140.2 ± 6.3 N/mm, for deformed and normal phenotypes, respectively). The bone profiles based on μCT observations showed no difference in the osteoclastic resorption while no difference in matrix production was observed between deformed (total bone area 5442.0 ± 110.1 μm2) and normal vertebrae (total bone area 5931.2 ± 249.8 μm2) in this study. Consequently, the lower P content rather results from a reduced degree of mineralization in the deformed phenotype. Finally, we quantified differential gene expression between deformed vertebrae (pronounced biconcave) and normal phenotype by employing deep RNA-sequencing and mapping against a reference bone transcriptome for rainbow trout. In total, 1289 genes were differentially expressed. Among them, in deformed fish we observed that BGLAP, MGP and NOG, an inhibitor of BMP signalling pathway, were up-regulated while COL11a1 was down-regulated. These genes are central actors involved in the reduced degree of mineralization triggering vertebral deformities. These results will further the understanding of P deficiency-induced deformities; hence providing new tools for improved P management in production setting

show abstract

Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

J¹,

MH²

2015

Next Generat Sequenc & Applic

View full text Add to dashboard Cite

show abstract

“…This has resulted in a comprehensive insight into bone matrix and cell biology; moreover, functional ‐omic studies have identified critical players in normal bone formation and remodelling (Le Luyer et al . ). The preferred method for fish skeletal deformity diagnostics has for long been radiography.…”

Section: Methodological Considerationsmentioning

confidence: 97%

“…The use of in vitro studies is an alternative method applied to further unravel the underlying mechanisms of bone development (Le Luyer et al . ). Some obvious advantages of such methods over experiments conducted in vivo include the ability to have more control on the cells being assessed and their environment, the relative ease of sampling and analysing changes in the parameters applied under investigation and the ability to reduce animal experiments.…”

Section: Methodological Considerationsmentioning

confidence: 97%

Mineral nutrition and bone health in salmonids

Bæverfjord

Prabhu

Fjelldal

et al. 2018

Reviews in Aquaculture

View full text Add to dashboard Cite

In the recent years, challenges faced in salmonid farming with regard to deformities have helped to improve our knowledge on skeletal biology and development of bone health in salmonids. Different nutritional, genetic and environmental factors are associated with skeletal deformities in salmonids and other fish species. Minerals are a group of essential nutrients having a vital role to play in skeletal development, growth and remodelling. The knowledge generated thus far on the structural and functional importance of minerals in salmonid bone health is largely restricted to phosphorus. A brief account of dietary phosphorus-related bone deformities encountered in salmonids, critical life stages for the development of bone deformities, recent developments in the understanding of their aetiology and dietary phosphorus levels required to improve bone health are presented. The effect of increased dietary level of phosphorus in reducing bone health disorders under conditions of improved feed conversion efficiency and use of triploids in salmon farming are illustrated with help of metadata analysis from literature. With regard to the role of other essential minerals (macro-and micro-) impacting bone health in salmonids, data available are extremely limited and hence information on other fish species and mammals is summarized in relation to bone development and incidence of deformities. The need to improve mineral bioavailability, utilization and reduce effluent mineral load is presented in brief with example from Norwegian salmon farming. Refinements in mineral requirement recommendations for salmonids and advanced methodologies for studying aetiology of skeletal anomalies, bone mineral status, skeletal development and deformities are also discussed.

show abstract

mRNA and microRNA transcriptomics analyses in intermuscular bones of two carp species, rice flower carp (Cyprinus carpio var. Quanzhounensis) and Jian carp (Cyprinus carpio var. Jian)

Peng

Zeng

et al. 2019

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

Establishment of a comprehensive reference transcriptome for vertebral bone tissue to study the impacts of nutritional phosphorus deficiency in rainbow trout (Oncorhynchus mykiss, Walbaum)

Cited by 4 publications

References 11 publications

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

RNA-Seq Transcriptome Analysis of Pronounced Biconcave Vertebrae: A Common Abnormality in Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fed a Low-Phosphorus Diet

Mineral nutrition and bone health in salmonids

mRNA and microRNA transcriptomics analyses in intermuscular bones of two carp species, rice flower carp (Cyprinus carpio var. Quanzhounensis) and Jian carp (Cyprinus carpio var. Jian)

Contact Info

Product

Resources

About