Establishment of a Cell Line From Embryonic Tissues of the Fleshfly, Sarcophaga Peregrina (Insecta: Diptera)

Abstract

A continuous cell line was established from embryonic tissues of the fleshfly, Sarcophaga peregrina, and was designated as NIH-SaPe-4. The primary culture was initiated in October, 1977. and the cell line was passed 68 times during the following year. The cells were heterogeneous in morphology. Most cells were diploid and their chromosomes consisted of 4 metacentric, 6 submetacentric and 2 micro chromosomes. The population doubling time of the cell line was about 30 hr. The cells grew faster in Mitsuhashi-Maramorosch's medium than in Schneider's medium. The cells were either stored in the usual medium at 5°C for about 3 months, or in a medium containing 10% glycerol at -880°C for a longer period. Cell growth was suppressed by 20-hydroxyecdysone when at a greater concentration than 0.01 ,ug/ml, whereas insulin showed no effects on cell growth at a strength of 0.4 and 0.04 IU/ml.

In vitro analysis of venom from the wasp Nasonia vitripennis: Susceptibility of different cell lines and venom-induced changes in plasma membrane permeability

“…The focus of most of our work is with an embryonic cell line (NIH SaPe4; Takahashi et al, 1980) derived from the flesh fly, Sarcophaga peregrina (Diptera: Sarcophagidae). These cells are highly sensitive to the venom and the bi-and muhipolar morphology of adherent cells has permitted tracking of chronological changes in cell morphology induced by the venom.…”

Two new cell lines originated from the embryos of Clostera anachoreta (Lepidoptera: Notodontidae): characterization and susceptibility to baculoviruses

“…Karyologic analysis of the two cell lines was carried out at passage 15 according to Takahashi et al (1980). The chromosome number in CAF-Clan I and CAF-Clan II at passage 15 varied from 62 to 100 in the majority of cells, though a few cells exceeded 260 (n=30; Saitoh 1960), as shown in Fig.…”

Preliminary formulation of a chemically defined medium for insect cell cultures

“…As the assay cell line, NIH-SaPe-4 cell line [3] was mainly used, because it could be cultured in serum-free MM medium, and the growth was vigorous. After examining various media combinations, a 1: 1 mixture of MGM-443 and TC-199 media plus a mixture of lipids and lipid soluble substances was found to support the growth of the cell line.…”

“…However, this medium is not commercially available, and is purported to be difficult to prepare, and some components were hardly soluble. When I attempted to prepare this medium, I also experienced the same problem, and the soluble part alone could not support the growth of insect cell lines from Sarcophaga peregrina [3], Aedes albopictus [4], Papilo xuthus [5], and Mamestra brassicae [6] (Mitsuhashi, unpublished).…”

“…Fortunately, I found that a flesh fly, S. peregrina, cell line, NIHSaPe-4 [3], which had been cultured continuously in serum-free MM medium [7] could continue to multiply in the 1: 1 mixture of a chemically defined MGM-443 medium [8] and TC-199 medium [9] without serum, but with a mixture of lipids and lipid soluble substances added. Then based upon the components of this mixture, I could formulate a chemically defined medium which supports the continuous culture of the NIH-SaPe-4 cell line.…”