Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

Osaki, Tomohiro; Sunden, Yuji; Sugiyama, Akihiko; Aso, Kazuo; Murahata, Yusuke; Tsuka, Takeshi; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu

doi:10.4142/jvs.2016.17.3.385

Cited by 32 publications

(29 citation statements)

References 21 publications

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“…One interesting finding in our transcriptome profile was the overexpression of miRNA precursor genes such as cf‐mir‐374b , cf‐mir‐145 and cf‐mir‐23b . Indeed, cfa‐mir‐145 had already been described in a primary culture of canine mammary cancer, where it was found 993.4‐fold over normal mammary gland tissue expression . The mir‐374b was associated to malignant behaviour in human gastric cancer and was suggested as a potential prognostic marker in cervical cancer, while mir‐23b was found downregulated in human colon cancer maybe acting as a modulator suppressor of metastasis .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines

Cordeiro

Xavier

Rochetti

et al. 2017

Vet Comparative Oncology

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The prevalence of cancer in animals has increased significantly over the years. Mammary tumours are the most common neoplasia in dogs, in which around 50% are presented in the malignant form. Hence, the development and characterization of in vitro models for the study of canine tumours are important for the improvement of cancer diagnosis and treatment. Thus, the aim of this study was to characterize cell lines derived from canine mammary gland neoplasias which could be further used for basic and applied oncology research. Samples of canine mammary carcinomas were taken for cell culture and 2 cell lines were established and characterized in terms of cell morphology, tumourigenicity and global gene expression. Both cell lines presented spindle-shape morphology and shown common malignant features as in vitro invasion potential and expression of epithelial and mesenchymal proteins. Also, we found gene expression patterns between the 2 cell cultures in comparison to the normal mammary gland tissue. Cells from M25 culture showed a higher invasion and in vivo tumourigenic potential, associated to the overexpression of genes involved in focal adhesion and extracellular matrix communication, such as FN1, ITGA8 and THBS2. The phenotypic characterization of these cells along with their global gene expression profile potentially determine new therapeutic targets for mammary tumours.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines

Cordeiro

Xavier

Rochetti

et al. 2017

Vet Comparative Oncology

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“…In veterinary medicine, a few analyses of the miRNA expression levels in tissues of canine mammary tumors (CMT) have been published [ 119 , 120 , 123 , 124 ]. In one study, nine of ten miRNAs studied (miR-15a, miR-16, miR-17-5p, miR-21, miR-29b, miR-125b, miR-145, miR-155, miR-181b, let-7f) that are involved in human breast cancer appeared to have the same expression pattern in CMTs [ 119 ].…”

Section: Mirnamentioning

confidence: 99%

Current biomarkers of canine mammary tumors

et al. 2018

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Mammary tumors are the second most common neoplasia in dogs. Due to the high similarity of canine mammary tumors (CMT) to human breast cancers (HBC), human biomarkers of HBC are also detectable in cases of CMT. The evaluation of biomarkers enables clinical diagnoses, treatment options and prognosis for bitches suffering from this disease. The aim of this article is to give a short summary of the biomarkers of CMT based on current literature. Very promising biomarkers are miRNAs, cancer stem cells, and circulating tumor cells, as well as mutations of the breast cancer 1 gene (BRCA1) and breast cancer 2 gene (BRCA2). Until now, the most studied and reliable biomarkers of CMT have remained antigen Ki-67 (Ki-67), endothelial growth factor receptor, human epidermal growth factor receptor 2 (HER-2), estrogen receptor, progesterone receptor and cyclooxygenase 1 (COX-2), which can be detected in both serum and tissue samples using different molecular methods. However, carcinoembryonic antigen and cancer antigen 15-3 (CA 15-3), while poorly studied, seem to be good biomarkers, especially for the early detection and prognosis of CMT. We will also mention the following: proliferative cell nuclear antigen, tumor protein p53 (p53), E-cadherin, vascular endothelial growth factor, microRNAs, cancer stem cells and circulating tumor cells, which can also be useful biomarkers. Although many studies have been conducted so far, the estimation of biomarkers in cases of CMT is still not a common practice, and more detailed research should be done.

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“…Other in vitro studies have suggested miR‐143 and miR‐138a are dysregulated in some CMT cells, and that miR‐141 plays a role in CMT development by inhibiting tumor suppressor INK4A. [ 15,16 ]…”

Section: Introductionmentioning

confidence: 99%

CirculatingmicroRNAas biomarkers of canine mammary carcinoma in dogs

Fish

Martinez‐Romero

DeInnocentes

et al. 2020

Veterinary Internal Medicne

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Background: Differentiating benign from canine malignant mammary tumors requires invasive surgical biopsy. Circulating microRNAs (miRNA) may represent promising minimally invasive cancer biomarkers in people and animals.Objectives: To evaluate the serum mRNA profile between dogs with and without mammary carcinoma, and to determine if any of these markers have prognostic significance.Animals: Ten healthy client-owned female dogs (5 intact, 5 spayed) and 10 dogs with histologically confirmed mammary carcinoma were included; 9 were client-owned, whereas 1 was a research colony dog.Methods: Retrospective study. Serum miRNA was evaluated by RNA deep-sequencing (RNAseq) and digital droplet PCR (dPCR).Expression of candidate biomarkers miR-18a, miR-19b, miR-29b, miR-34c, miR-122, miR-125a, and miR-181a was compared with clinical characteristics, including grade, metastasis, and survival.Results: 452 unique serum miRNAs were detected by RNAseq. Sixty-five individual miRNAs were differentially expressed (>±1.5-fold) and statistically significant between groups. Serum miR-19b (P = .003) and miR-125a (P < .001) were significantly higher in the mammary carcinoma group by dPCR. Both had high accuracy based on receiver operator characteristic area under the curve (0.930 for miR-125a; 0.880 for miR-19b). Circulating miR-18a by RNAseq was significantly higher in mammary carcinoma dogs with histologic evidence of lymphatic invasion (P = 0.03). There was no significant association with any miRNA and survival or inflammatory status. Conclusions and Clinical Importance: Circulating miRNAs are differentially expressed in dogs with mammary carcinoma. Serum miR-19b and miR-18a represent candidate biomarkers for diagnosis and prognosis, respectively. Abbreviations: AUC, area under the curve; CMT, canine mammary tumor(s); dPCR, digital droplet PCR; FAM, 6-carboxyfluorescein; miRs/miRNA, microRNA; qPCR, quantitative PCR; RNAseq, RNA deep-sequencing; ROC, receiver operator characteristic.

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Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

Cited by 32 publications

References 21 publications

Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines

Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines

Current biomarkers of canine mammary tumors

CirculatingmicroRNAas biomarkers of canine mammary carcinoma in dogs

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