Establishment of 15 Cancer Cell Lines From Patients With Lung Cancer and the Potential Tools for Immunotherapy

Sugaya, Masakazu; Takenoyama, Mitsuhiro; Osaki, Toshihiro; Yasuda, Manabu; Nagashima, Akira; Sugio, Kenji; Yasumoto, Kosei

doi:10.1378/chest.122.1.282

Cited by 57 publications

(59 citation statements)

References 23 publications

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“…Agarose gel electrophoresis showed only the DNA sample with mutation to reveal a band, under stringent PCR conditions. Next, we used a DNA derived from cell line (G603L) (Sugaya et al, 2002) with an L858R mutation confirmed by sequencing ( Figure 2B), as a positive control. To confirm the sensitivity of the MASA method, PCR was performed in the mixture of diluted mutation-positive DNA with wild-type DNA.…”

Section: Results Exon 19 and 21 Mutations In Egfr Gene By Screening Mmentioning

confidence: 99%

Mutations within the tyrosine kinase domain of EGFR gene specifically occur in lung adenocarcinoma patients with a low exposure of tobacco smoking

et al. 2006

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Somatically acquired mutations in the epidermal growth factor receptor (EGFR) gene in lung cancer are associated with significant clinical responses to gefitinib, a tyrosine kinase inhibitor that targets EGFR. We screened the EGFR in 469 resected tumours of patients with lung cancer, which included 322 adenocarcinomas, 102 squamous cell carcinomas, 27 large cell carcinomas, 13 small cell carcinomas, and five other cell types. PCR with a specific condition was performed to identify any deletion in exon 19, while mutantallele-specific amplification was performed to identify a mutation in codon 858 of exon 21. EGFR mutations were found in 136 cases (42.2%) with adenocarcinoma, in one case with large cell carcinoma, and in one case with pleomorphic carcinoma. An in-frame deletion in exon 19 was found in 62 cases while an L858R mutation was found in 77 cases. In the 322 cases with adenocarcinoma, these mutations were more frequently found in women than in men (P ¼ 0.0004), in well differentiated tumours than in poorly differentiated tumours (P ¼ 0.0014), and in patients who were never smokers than in patients who were current/former smokers (Po0.0001). The mutation was more frequently observed in patients who smoked p20 pack-year, and in patients who quit at least 20 years before the date of diagnosis for lung cancer. The K-ras mutations were more frequently found in smokers than in never smokers, and in high-dose smokers than in low-dose smokers. In conclusion, the mutations within the tyrosine kinase domain of EGFR were found to specifically occur in lung adenocarcinoma patients with a low exposure of tobacco smoking.

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Section: Results Exon 19 and 21 Mutations In Egfr Gene By Screening Mmentioning

confidence: 99%

Mutations within the tyrosine kinase domain of EGFR gene specifically occur in lung adenocarcinoma patients with a low exposure of tobacco smoking

et al. 2006

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“…In general, it is considered much more difficult to establish lung cancer cell lines in comparison with other types of epi- npg thelia-originated cell lines [28] . Chemically defined media such as ACL3, ACL4, and HITES were first used for primary lung cancer cell culture with success.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of primary lung cancer cell lines from Chinese population

Zheng

Sun

et al. 2011

Acta Pharmacol Sin

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Aim: To establish and characterize primary lung cancer cell lines from Chinese population. Methods: Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results: Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations. Conclusion: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.

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“…The cisplatin-resistant KB/CP4 and P/CDP6 cells were derived from KB and PC3 as described previously (Tanabe et al, 2003). Seven lung cancer cell lines were obtained as described previously (Sugaya et al, 2002). Cell lines were maintained in a 5% CO 2 atmosphere at 371C.…”

Section: Cell Culturementioning

confidence: 99%

ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes

et al. 2007

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Zinc-finger protein 143 (ZNF143) is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription. We previously showed that ZNF143 expression is induced by treatment with DNA-damaging agents and that it preferentially binds to cisplatin-modified DNA. In this study, the potential function of ZNF143 was investigated. ZNF143 was overexpressed in cisplatin-resistant cells. ZNF143 knockdown in prostate cancer caused increased sensitivity for cisplatin, but not for oxaliplatin, etoposide and vincristine. We also showed that ZNF143 is associated with tumor suppressor gene product p73 but not with p53. p73 could stimulate the binding of ZNF143 to both ZNF143 binding site and cisplatin-modified DNA, and modulate the function of ZNF143. We provide a direct evidence that both Rad51 and flap endonuclease-1 are target genes of ZNF143 and overexpressed in cisplatin-resistant cells. Taken together, these experiments demonstrate that an interplay of ZNF143, p73 and ZNF143 target genes is involved in DNA repair gene expression and cisplatin resistance.

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Establishment of 15 Cancer Cell Lines From Patients With Lung Cancer and the Potential Tools for Immunotherapy

Cited by 57 publications

References 23 publications

Mutations within the tyrosine kinase domain of EGFR gene specifically occur in lung adenocarcinoma patients with a low exposure of tobacco smoking

Mutations within the tyrosine kinase domain of EGFR gene specifically occur in lung adenocarcinoma patients with a low exposure of tobacco smoking

Establishment and characterization of primary lung cancer cell lines from Chinese population

ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes

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