Establishment by enzyme-linked immunosorbent assay of seronegative range for herpes simplex virus and cytomegalovirus antibodies and evaluation of heterologous responses to live varicella vaccine

Gong, Alice; Lipton, Susan; Brunell, Philip A.

doi:10.1128/jcm.26.4.781-783.1988

Cited by 2 publications

(2 citation statements)

References 16 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coated plates could be used for up to 3 months. The ELISA method used in our laboratory (8,21), including the assays for CMV and HSV-1 (16) and for VZV (28), has been described in detail elsewhere. The results were expressed as difference of optical density (DOD), which was calculated by subtracting the average optical density of two controls from the average of two antigen values multiplied by a thousand.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme-linked immunosorbent assay for detection of antibody to human herpesvirus 6

Chokephaibulkit

Brunell

Vimal

et al. 1997

Clin Diagn Lab Immunol

Self Cite

View full text Add to dashboard Cite

The results obtained with an enzyme-linked immunosorbent assay (ELISA) for detection of human herpesvirus 6 (HHV-6) immunoglobulin G using a single 1:100 dilution of serum correlated well with those found by an indirect fluorescence microscopic assay (IFA) (r ‫؍‬ 0.71). Concordant results were found in all 7 paired serum samples obtained from patients with acute primary infections and in 37 of 41 (90.24%) single serum samples. Fourteen serum samples (25%) which yielded nonspecific results by IFA were evaluable by ELISA. In a serologic survey using the ELISA, a disproportionate number of 12-month-old infants had low differenceof-optical-density values, suggesting that maternal antibody might persist beyond a year of age. This finding and the rises in antibody to HHV-6 found in patients with primary cytomegalovirus infections might lead to overestimation of HHV-6 infection rates in young children in seroprevalence studies. Other herpesvirus infections produced lesser effects on anti-HHV-6.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Establishment of the seronegative range. The method for determining the seronegative range has been described elsewhere (16). Twenty cord serum samples were treated with protein G (Quik-Sep IgM assay; Isolab, Inc., Akron, Ohio) twice to remove IgG.…”

Section: Methodsmentioning

confidence: 99%