Establishment and proteomic characterization of NCC-LMS1-C1, a novel cell line of primary leiomyosarcoma of the bone

Sakumoto, Marimu; Takahashi, Mami; Oyama, Rieko; Takai, Yoko; Kito, Fusako; Shiozawa, Kumiko; Qiao, Zhiwei; Yoshida, Akihiko; Kawai, Akira; Kondo, Tadashi

doi:10.1093/jjco/hyx096

Cited by 8 publications

(2 citation statements)

References 14 publications

(9 reference statements)

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“…The pathological examination of LMSB includes observing microscopic morphology and immunohistochemistry ( 19 ). Within LMSB lesions, long, narrow, spindle-shaped tumor cells form bundles within eosinophilic-rich cytoplasm and have cigar-shaped nuclei ( 23 , 24 ). Immunohistochemistry is of unique value in diagnosing myogenic tumors and must be conducted to accurately diagnose LMSB ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Case report and literature review: Primary leiomyosarcoma of the bone in the trochanteric region of the femur

Li²,

Cao³

et al. 2023

Front. Surg.

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BackgroundPrimary leiomyosarcoma of the bone (LMSB) is an extremely rare, invasive, and highly destructive primary osteosarcoma with limited treatment options and poor prognosis. Only a few case reports of LMSB have been described because of its rarity. Therefore, clinicians have a limited understanding of its diagnosis, treatment, and prognosis, and the final diagnosis depends on histopathological findings. In this report, we describe a rare case of primary LMSB in the trochanteric region of the femur. Reporting this case may increase the dissemination and understanding of information regarding LMSB and provide a reference for the diagnosis and treatment of similar cases.Case presentationA 63-year-old woman presented with pain and limited movement of the left hip, which had lasted for 3 months, with no history of trauma or illness. Plain radiography and computed tomography revealed a solitary osteolytic lesion in the trochanteric area of the left femur with focal cortical destruction. Magnetic resonance imaging findings suggested invasion of the lesion into the bone cortex, forming a soft tissue mass, although no distant positive findings were observed on a whole-body bone scan. A bone tumor puncture biopsy was performed to obtain a final diagnosis, and histopathological evaluation revealed left femoral intertrochanteric leiomyosarcoma, classified as G1T2M0 and staged as IB (extracompartmental low-grade malignant) according to the Enneking staging system. Thus, we performed extensive debridement and left hip arthroplasty. Postoperative chemotherapy was administered, and the patient was followed up for 4 years. Four years later, the patient's left hip pain had resolved, joint activity was good, and no signs of recurrence or distant metastasis of the bone tumor were noted.ConclusionFor proximal femoral Enneking stage IB LMSB, extensive tumor resection combined with tumor prosthesis replacement may be an effective treatment method to prolong the patient's lifespan and to restore joint function.

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Section: Discussionmentioning

confidence: 99%

Case report and literature review: Primary leiomyosarcoma of the bone in the trochanteric region of the femur

Li²,

Cao³

et al. 2023

Front. Surg.

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“…It remains however unclear how well these preclinical tumor models recapitulate patient tumor biology. We therefore developed sarcoma patient‐derived models for proteomic and functional studies, and tried to assess whether PDXsretain their proteomic characteristics with successive passaging …”

Section: Introductionmentioning

confidence: 99%

Species‐Specific Quantitative Proteomics Profiles of Sarcoma Patient‐Derived Models Closely Reflect Their Primary Tumors

Shiozawa¹,

Oyama²,

Takahashi³

et al. 2019

Proteomics Clinical Apps

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Purpose The purpose is to examine whether patient‐derived sarcoma models recapitulate the spectrum of sarcoma heterogeneity seen in patients. Experimental design To characterize patient‐derived models for functional studies, proteomic comparisons with originating sarcomas representative of three intrinsic subtypes by MS are performed. Results Human protein profiling is found to be retained with high fidelity in patient‐derived models. The protein profiles of patient sarcoma tumors and mouse stroma are characterized by species‐specific quantitative proteomics. Protein‐expression levels in mouse stroma are affected by the primary human tumor. The levels of stromal proteins derived from tumors are lower in PDXs and cell lines, and some human stromal proteins are replaced by the corresponding mouse proteins in PDXs. Conclusions and clinical relevance These findings suggest that the effects of the microenvironment on drug responses may not reflect those in the primary tumor. This cross‐species proteomic analysis in PDXs can potentially improve preclinical evaluation of treatment modalities and enhance the ability to predict clinical trial responses.

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Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1

Oyama¹,

Kito²,

Sakumoto³

et al. 2018

In Vitro Cell.Dev.Biol.-Animal

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Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18-SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18-SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18-SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene.

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Establishment and proteomic characterization of NCC-LMS1-C1, a novel cell line of primary leiomyosarcoma of the bone

Cited by 8 publications

References 14 publications

Case report and literature review: Primary leiomyosarcoma of the bone in the trochanteric region of the femur

Case report and literature review: Primary leiomyosarcoma of the bone in the trochanteric region of the femur

Species‐Specific Quantitative Proteomics Profiles of Sarcoma Patient‐Derived Models Closely Reflect Their Primary Tumors

Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1

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