Establishment and Maintenance of Cell Polarity in the C. elegans Intestine

Bossinger, Olaf; Wiesenfahrt, Tobias; Hoffmann, Michael

doi:10.1007/978-3-319-14466-5_2

Cited by 3 publications

(2 citation statements)

References 206 publications

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“…Ce AJs are fully established during the so-called bean and comma stages (names attributed to the early elongation stages based on the shape of the embryo; Sulston et al, 1983 ). C. elegans possesses a single type of apical junction that comprises two adjacent adhesion systems: AS-I and AS-II ( Bossinger et al, 2015 ). AS-I is composed of a cadherin-catenin complex (CCC), constituted by HMR-1/E-cadherin, HMP-1/α-catenin, HMP-2/β-catenin and JAC-1/p120-catenin, which links to intermediate filaments of the cytoskeleton and F-actin ( Costa et al, 1998 ; Pettitt et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

et al. 2021

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mRNA localization is an evolutionarily widespread phenomenon that can facilitate subcellular protein targeting. Extensive work has focused on mRNA targeting through “zip-codes” within untranslated regions (UTRs), while much less is known about translation-dependent cues. Here, we examine mRNA localization in Caenorhabditis elegans embryonic epithelia. From an smFISH-based survey, we identified mRNAs associated with the cell membrane or cortex, and with apical junctions in a stage- and cell type-specific manner. Mutational analyses for one of these transcripts, dlg-1/discs large, revealed that it relied on a translation-dependent process and did not require its 5’ or 3'UTRs. We suggest a model in which dlg-1 transcripts are co-translationally localized with the nascent protein: first the translating complex goes to the cell membrane using sequences located at the carboxy-terminus/3’ end, and then apically using amino-terminal/5’ sequences. These studies identify a translation-based process for mRNA localization within developing epithelia and determine the necessary cis-acting sequences for dlg-1 mRNA targeting.

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Section: Introductionmentioning

confidence: 99%

Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

et al. 2021

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“…Many factors contribute to the onset and establishment of this asymmetric segregation of proteins, including the trafficking and turnover of active Cdc42, actomyosin organization, asymmetric distribution of specific phospholipids, adhesion junctions and mutual antagonism (Jiang et al, 2015;Jewett and Prekeris, 2018;Motegi et al, 2020). In C. elegans, intestinal cells acquire apicobasal polarity, which is crucial for formation of the interior lumen (Leung et al, 1999;Bernadskaya et al, 201;Shafaq-Zadah et al, 2012;Rasmussen et al, 2012;Bossinger et al 2015;Asan et al, 2016). Epidermal cells also have apicobasal polarity, which is reinforced by adhesion junction components that connect neighboring cells (Patel et al, 2008;Patel and Soto, 2013).…”

Section: Introductionmentioning

confidence: 99%

Multi-tissue patterning drives anterior morphogenesis of theC. elegansembryo

Grimbert

Mastronardi

Christensen

et al. 2020

Preprint

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Complex structures derived from multiple tissue types are challenging to study in vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical and mechanical cues between cells from two different tissues can govern specific morphogenetic events. Here we usedCaenorhabditis elegans as a model system to show how cells from three different tissues are coordinated to give rise to the anterior lumen. This poorly understood process has remained a black box for embryonic morphogenesis. Using various microscopy and software approaches, we describe the movements and patterns of epidermal cells, neuroblasts and pharyngeal cells that contribute to lumen formation. The anterior-most pharyngeal cells (arcade cells) may provide the first marker for the location of the future lumen and facilitate the patterning of the surrounding neuroblasts. These neuroblast patterns control the rate of migration of the anterior epidermal cells, whereas the epidermal cells ultimately reinforce and control the position of the future lumen, as they must join with the pharyngeal cells for their epithelialization. Our studies are the first to characterize anterior morphogenesis in C. elegans in detail and should lay the framework for identifying how these different patterns are controlled at the molecular level.

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Translation-dependent mRNA localization toCaenorhabditiselegans adherens junctions

Tocchini

Rohner

Stetina

2021

Preprint

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mRNA localization is an evolutionarily widespread phenomenon that facilitates sub-cellular protein targeting. Extensive work has focused on mRNA targeting through “zip codes” within untranslated regions (UTRs), while much less is known about translation-dependent cues. Here, we examine mRNA localization in Caenorhabditis elegans embryonic epithelia. From an smFISH-based survey, we identified mRNAs associated with the cell membrane or cortex, and with apical junctions in a stage- and cell type-specific manner. Mutational analyses for one of these transcripts, dlg-1/discs large, revealed that it relied on a translation-dependent process and did not require its 5′ or 3′ UTR. We suggest a model in which dlg-1 transcripts are co-translationally colocalized with the encoded protein: first the translating complex goes to the cell membrane through sequences of the SH3 domain, and then to the apical junction by the L27 and PDZ sequences. In addition, the Hook and GuK sequences contribute to the second step: they are required for mRNA, but not protein, to accumulate at the apical junctions from locations at or near the membrane. These studies identify a translation-based process for mRNA localization within developing epithelia and determine the necessary cis-acting sequences for dlg-1 mRNA targeting.

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Establishment and Maintenance of Cell Polarity in the C. elegans Intestine

Cited by 3 publications

References 206 publications

Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

Multi-tissue patterning drives anterior morphogenesis of theC. elegansembryo

Translation-dependent mRNA localization toCaenorhabditiselegans adherens junctions

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