Establishment and growth of the rhizome of<i>Alstroemeria</i>as affected by temperature and the root system

Bond, S.; Alderson, P. G.

doi:10.1080/00221589.1993.11516423

Cited by 6 publications

(1 citation statement)

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“…Similar conditions have been recommended for shoot induction in floral buds of Freesia and for pedicel explants of Eucharis grandiflora and Nerine bowdenii (Pierik, 1990). According to Bond and Alderson (1993a), vernalization in Alstroemeria can be achieved under greenhouse conditions by exposing plants to 5°C for 6-8 weeks or to 13°C for 16 weeks.…”

Section: Discussionmentioning

confidence: 95%

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

Pedraza‐Santos

López-Peralta

Hernández

et al. 2005

Plant Cell Tiss Organ Cult

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The common techniques for the in vitro production of Alstroemeria plants are based on rhizomes as explants, which have low multiplication rates and a high risk of carrying viral diseases. To overcome these problems, we developed a protocol for the in vitro regeneration of Alstroemeria cv.'Yellow King', by testing for shoot induction several explant sources (leaf, stem apices, rhizomes and immature inflorescence apices), temperature and light/dark regimes, hormone and salt concentrations. For shoot multiplication and rooting, several hormone concentrations were tested. We found that only the young floral apices produced adventitious shoots by direct organogenesis. The highest shoot induction rate (10.4 shoots per explant) was obtained by incubation in the dark for 15 days at 8°C followed by 15 days at 25°C and a 16-h/8-h light/ dark regime, on a Murashige and Skoog (1962) liquid medium at 50% of the salt concentration, supplemented with 2.5 mg l )1 KIN, 1.5 mg l )1 BA and 1.0 mg l )1 NAA, using a piece filter paper to support the explant. The highest shoot multiplication rate (9 shoots per explant) was obtained on a liquid MS medium at full strength supplemented only with BA at 1.0 mg l )1 . In vitro rooting of shoots was induced also on a liquid MS medium, either with or without plant hormones.

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Section: Discussionmentioning

confidence: 95%