Establishment and characterization of unique cell lines derived from pyothorax‐associated lymphoma which develops in long‐standing pyothorax and is strongly associated with Epstein‐Barr virus infection

Takakuwa, Tetsuya; Luo, Wen; Ham, Maria Francisca; Mizuki, Masao; Iuchi, Keiji; Aozasa, Katsuyuki

doi:10.1111/j.1349-7006.2003.tb01367.x

Cited by 25 publications

(29 citation statements)

References 19 publications

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“…In the previous studies, shortening of poly A tracts within the sequence of ATR genes was described in sporadic stomach cancer with mutation of mismatch repair (MMR) genes, 36 whereas such a mutation was not observed in OPL-3, which is the only PAL line showing mismatch repair phenotype. 5 Further study is necessary to evaluate whether the mutation of ATR is frequent in lymphoid malignancies with mismatch repair phenotype or not.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][7] Characteristics of these eight lines are summarized in Table 1. These lines were established from eight patients with PAL, with a 39-66 year history of chronic pyothorax resulting from artificial pneumothorax for the treatment of pulmonary tuberculosis or tuberculous pleuritis.…”

Section: Cell Linesmentioning

confidence: 99%

“…Until now, at least eight cell lines derived from PAL tissues have been established. [4][5][6][7] These lines could be used for studying the effect of chronic inflammation in lymphomagenesis.…”

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confidence: 99%

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Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma

Liu

Takakuwa

Fujita

et al. 2005

Laboratory Investigation

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Pyothorax-associated lymphoma (PAL) is non-Hodgkin's lymphoma that develops from chronic inflammation. Free radicals and oxidative stress generated in the inflammatory lesions could cause DNA damage and thus provide a basis for lymphomagenesis. Ataxia-telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) genes are responsive genes for DNA damage, therefore potential involvement of these genes in PAL lymphomagenesis was examined in eight PAL cell lines and clinical samples from five cases. ATM mutations were detected in five of eight PAL lines. All but one of these mutations affected the phosphatidylinositol 3-kinase domain, indicating the loss-of-function mutation of ATM gene. Heterozygous mutations of ATR were found in two of eight lines; one a missense and the other a truncation mutation. ATR mutations were also detected in two of five cases in clinical samples from PAL. PAL cells with ATR mutation showed a delay or abrogation in repair for ionizing radiation (IR)-induced DNA double-strand breaks (DSBs) or ultraviolet (UV)-induced DNA single-strand breaks (SSBs), and exhibited a defect in p53 accumulation and failure in cell cycle checkpoint at G1-S phase. These findings showed that mutations of ATR gene result in failure for DNA DSB and SSB repair, suggesting the role of ATM and ATR gene mutations in PAL lymphomagenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Linesmentioning

confidence: 99%

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Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma

Liu

Takakuwa

Fujita

et al. 2005

Laboratory Investigation

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“…13 In situ hybridization with an EBER-1 probe revealed positive signals in the nucleus of tumor cells in all PAL cases. 8,9 Amplification and sequencing of rearranged IgV H and IgV L genes…”

Section: Clinical Casesmentioning

confidence: 99%

Cell origin of pyothorax-associated lymphoma: a lymphoma strongly associated with Epstein–Barr virus infection

et al. 2007

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“…Although the vast majority of individuals harbouring EBV spend their lives as asymptomatic carriers, latent infections may induce endemic Burkitt's lymphoma, nasopharyngeal carcinoma (NPC), gastric carcinoma (GC), NK/T-cell lymphoma, pyothorax-associated lymphoma (PAL), chronic active EBV infection (CAEBV) and so on (Rickinson & Kieff, 2001;Imai et al, 1998;Takakuwa et al, 2003). The latency-associated genes encode six nuclear antigens (EBNAs) and three latent membrane proteins (LMPs).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Epstein–Barr virus BamHI-A rightward transcripts and their expression patterns in lytic and latent infections

Yamamoto

Iwatsuki

2012

Journal of Medical Microbiology

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Epstein-Barr virus (EBV)BamHI-A rightward transcripts (BARTs; also designated complementary strand transcripts or CSTs) have been demonstrated to contain several splicing forms in EBV-infected cells. To date, however, little is known about the actual full-length splicing form and its functions. In the present study, we proved that six forms of BARTs were present in EBV-positive cell lines and various tissue specimens with different EBV infection patterns. Of the BART-encoded genes, mRNA of four major splicing forms, including BARF0, RPMS1, RPMS1A and A73, were expressed in all EBV-infected cells. On the other hand, mRNA of two minor splicing forms, RK-BARF0 and RB3, was rarely detected, or if at all, at very low expression levels. Both RPMS1A and RPMS1 mRNA was transcribed at higher levels in EBV-infected cells. In particular, RPMS1 mRNA was expressed abundantly in epithelial carcinoma cells, including gastric carcinoma and nasopharyngeal carcinoma, in association with a lytic infection signal, BZLF1 mRNA. The four major splicing forms were expressed much less in B-cell lines with an integrated EBV genome than in those with episomal EBV genomes. These data indicate that at least six splicing forms can be expressed by EBV-infected cells or tissues, although the expression patterns or levels differ for different infection states such as lytic and latent infections.

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Establishment and characterization of unique cell lines derived from pyothorax‐associated lymphoma which develops in long‐standing pyothorax and is strongly associated with Epstein‐Barr virus infection

Abstract: alignant lymphoma frequently develops in the pleural cavity of patients with over 20 years' history of pyothorax.

Cited by 25 publications

References 19 publications

Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma

Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma

Cell origin of pyothorax-associated lymphoma: a lymphoma strongly associated with Epstein–Barr virus infection

Diversity of Epstein–Barr virus BamHI-A rightward transcripts and their expression patterns in lytic and latent infections

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