Establishment and Characterization of Three New Cell Lines Derived from the Ascites of Human Ovarian Carcinomas

Microarray analysis of gene expression mirrors the biology of an ovarian cancer model

“…Most human ovarian cancer cell lines described were derived from ascites or pleural e usion (Alama et al, 1996;Bast et al, 1981;Buller et al, 1995;DiSaia et al, 1975;Fogh and Trempe, 1975;Golombick and Bezwoda, 1991;Golombick et al, 1990;Grunt et al, 1993;Hamilton et al, 1983;Hirte et al, 1994;Langdon et al, 1988;Provencher et al, 1993;Sinna et al, 1979;Wong et al, 1990;Yamada et al, 1991), with only a few cell lines derived from primary ovarian solid tumors (Crickard et al, 1989;Langdon et al, 1988;Woods et al, 1979) or metastases (Buick et al, 1985). In addition, most cell lines originate from tumor material obtained following adjuvant therapy such as chemotherapy or radiation therapy, which could introduce confounding genetic events.…”

Stromal cell-derived factor-1α (SDF-1α/CXCL12) stimulates ovarian cancer cell growth through the EGF receptor transactivation

“…OC 314, OC 315, and OC 316 were established from human ovarian carcinoma malignant ascites as previously described [16]. The human ovarian cancer cell line IGROV was obtained from the Interlab Cell Line Collection (Genova, Italy).…”

Conversion of a tumor-binding peptide identified by phage display to a functional chimeric T cell antigen receptor

“…The plasmid DNA was precipitated with a 1:10 volume of 3 M sodium acetate and 2.5 volume of ethanol (EtOH), washed in 70% EtOH and resuspended in sterile distilled H 2 O. Linearized Bpep-CAR DNA was stored at À201C until used for electroporation. Low passage primary human ovarian cancer cell lines (RSN001 and RSN002) were established in the laboratory according to previously published methods by Alama et al 10 Ascites was collected from women with ovarian cancer who were undergoing an exploratory laparotomy. The ascites was centrifuged at 1200 r.p.m.…”