“…Se4 cells (BCIRL/AMCY-SeE-CLG4), originated from embryos of the beet armyworm S. exigua (Goodman et al, 2001) were maintained at 27 C in Ex-Cell Ò 420 medium (JRH Biosciences, Hampshire, UK), supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Sigma Co., Bornem, Belgium). Se4 cell lines resistant to 20E (Se4-20E-R4) or methoxyfenozide (Se4-RH2485-R4) were grown in the presence of 100 mM of their respective ecdysteroid agonist .…”