Establishment and characterization of C-type RNA virus-producing cell lines from radiation-induced murine osteosarcomas

Abstract

Eight cell lines were established from murine osteosarcomas induced in vivo with the radionuclides 224Ra and 227Th. They have been compared by light and electron microscopy, by karyology, and by their growth properties. The morphology, the growth pattern, and the ability to induce tumors in mice indicate that five of them are tumor cell lines. Chromosome studies demonstrated that the five cell lines have marker chromosomes. The other cell lines only showed some criteria generally used to score for transformation of fibroblasts and they may be derived from stromal cells. All cell lines release virus particles in the culture fluid which have the typical properties of RNA tumor viruses. They possess C-type morphology, a density of 1.16--1.18 g/cm3, a 60--70 S RNA, a RNA dependent DNA polymerase and they induce syncytia in rat XC cells. The possible significance of these virus particles in radiation osteosarcomagenesis is discussed.

Establishment and characterization of osteogenic cell lines from a spontaneous murine osteosarcoma

“…After 14 days in culture K 7 and K 8 cells developed small compact colonies with little variation in size, K 12 and K 14 cells large compact colonies, and K 37 cells large, loosely packed colonies. None of the soft agar colonies formed by these cells reached the size of those observed after seedidng 0s-5 cells, a permanent cell line established from a radiation-induced mouse osteosarcoma which was used as a control [12].…”

“…None of the soft agar colonies formed by these cells reached the size of those observed after seedidng 0s-5 cells, a permanent cell line established from a radiation-induced mouse osteosarcoma which was used as a control [12]. The size and morphology of the colonies, however, varied considerably.…”

Amplification of endogenous proviral MuLV sequences in radiationinduced osteosarcomas

“…In addition, retroviruses have frequently been detected in spontaneous and radiation-induced murine bone tumors (Finkel and Biskis, 1969; Erfle and Marquart, 1978) as well as in cell lines established from radiation-induced osteosarcomas (Erfle et al, 1979).…”

The Influence of Paramunisation, Tumor Cell Vaccination and Whole Body Irradiation on the Growth of Radiation‐induced Murine Osteosarcoma

“…Exp 1,5,6,7;. Tables 5,6) and was relatively constant except for three experiments where the TD,, was five times higher (2.5 x 104 cells) (Table 4, Exp. 2, 3,4).…”

“…Turnour Cell Vaccine: Passage no. 170 of the permanent cell line 0s-2, originating from a **'Radium induced osteogenic sarcoma in a female C3H x 101/F, hybrid mouse was used for the immunization studies (6). The cells induce sarcomas in newborn C 3 H X 101/F, mice, but not in adult mice and they produce a murine C-type retrovirus.…”