Establishment and characterization of atrazine degrading cultures from Nigerian agricultural soil using traditional and Bio-Sep bead enrichment techniques

Omotayo, Ayodele E.; Ilori, Matthew O.; Amund, Olukayode O.; Ghosh, Debasish; Roy, Krishnakali; Radosevich, Mark

doi:10.1016/j.apsoil.2011.01.006

Cited by 16 publications

(7 citation statements)

References 66 publications

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“…It is also conceivable that 14 C-labelled atrazine may sorb to the activated carbon Bio-Sep bead and thus become biologically unavailable for biodegradation. However, previously published experiments with atrazine and Bio-Sep beads do not suggest that atrazine sorption on the beads retards biomineralization (Ghosh et al, 2009;Omotayo et al, 2011). …”

Section: Measurement Of Atrazine Mineralization With [U-ring-14 C]-atmentioning

confidence: 89%

“…The beads were made of granular activated carbon compressed into size and shape of lentils (Sublette et al, 1996;Peacock et al, 2004;Williams et al, 2013). The technique is based on sorption of atrazine in aqueous solution on the beads followed by in-situ incubation to enrich for atrazine-degrading bacteria, possibly in the form of biofilm growth (Mehta et al, 2004;Peacock et al, 2004;Ghosh et al, 2009;Omotayo et al, 2011). The beads were equilibrated in solution of 2 mg atrazine/l or, for control beads, the same volume of distilled water for at least three days.…”

Section: Samplingmentioning

confidence: 99%

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Molecular analysis of atrazine-degrading bacteria and catabolic genes in the water column and sediment of a created wetland in an agricultural/urban watershed

Douglass

Radosevich

Tuovinen

2015

Ecological Engineering

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Section: Measurement Of Atrazine Mineralization With [U-ring-14 C]-atmentioning

confidence: 89%

Section: Samplingmentioning

confidence: 99%

Molecular analysis of atrazine-degrading bacteria and catabolic genes in the water column and sediment of a created wetland in an agricultural/urban watershed

Douglass

Radosevich

Tuovinen

2015

Ecological Engineering

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“…Selected substrates can be attached to the surfaces of the beads by covalent bonding via a proprietary method that enables microbial access to substrates while preventing leaching. Bio-Sep beads have been used in aquatic systems (Anderson et al, 2003 ; Peacock et al, 2004 ; Sublette et al, 2006 ; Baldwin et al, 2008 ; Williams et al, 2013 ) as well as terrestrial systems (DeAngelis et al, 2011 ; Omotayo et al, 2011 ) to monitor microbial activity. Bead pouches used in this study consisted of 5 g (~20 ml) of Kraft lignin amended or unamended beads in an 8 cm diameter circular window screen mesh pouch (Phifer silver gray fiberglass screen, product 4788811608, approximately 1 mm mesh size) that was heat-sealed.…”

Section: Methodsmentioning

confidence: 99%

Two decades of warming increases diversity of a potentially lignolytic bacterial community

2015

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As Earth's climate warms, the massive stores of carbon found in soil are predicted to become depleted, and leave behind a smaller carbon pool that is less accessible to microbes. At a long-term forest soil-warming experiment in central Massachusetts, soil respiration and bacterial diversity have increased, while fungal biomass and microbially-accessible soil carbon have decreased. Here, we evaluate how warming has affected the microbial community's capability to degrade chemically-complex soil carbon using lignin-amended BioSep beads. We profiled the bacterial and fungal communities using PCR-based methods and completed extracellular enzyme assays as a proxy for potential community function. We found that lignin-amended beads selected for a distinct community containing bacterial taxa closely related to known lignin degraders, as well as members of many genera not previously noted as capable of degrading lignin. Warming tended to drive bacterial community structure more strongly in the lignin beads, while the effect on the fungal community was limited to unamended beads. Of those bacterial operational taxonomic units (OTUs) enriched by the warming treatment, many were enriched uniquely on lignin-amended beads. These taxa may be contributing to enhanced soil respiration under warming despite reduced readily available C availability. In aggregate, these results suggest that there is genetic potential for chemically complex soil carbon degradation that may lead to extended elevated soil respiration with long-term warming.

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“…Atrazine is the most widely used chloro-s-triazine globally and has been detected in various water bodies and groundwater throughout South Africa since the 1980′s (Ansara- Ross et al, 2012;Takáts et al, 2001). Its use is banned in Europe since 2004 due to its relative persistence and risk of water contamination, though the use of the other triazine herbicides is still permitted (Ackerman, 2007;Hang et al, 2005;Herranz et al, 2008;Lin et al, 2011;Omotayo et al, 2011;Wang and Xie, 2012). Atrazine is however still used extensively in China, USA, India, South America, Africa and Australia (Ansara- Ross et al, 2012;Liu et al, 2016;Siddiqua et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation of chloro-s-triazines in the Hartbeespoort Dam catchment, South Africa

Rimayi

Odusanya²,

Weiss

et al. 2018

Science of The Total Environment

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Seasonal variation of eight chloro-s-triazine herbicides and seven major atrazine and terbuthylazine degradation products was monitored in the Hartbeespoort Dam catchment using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS). Lake, river and groundwater were sampled from the Hartbeespoort Dam catchment over four seasons and the downstream Jukskei River was monitored during the winter season. Triazine herbicide concentrations in the Hartbeespoort Dam were in the order atrazine>simazine>propazine>ametryn>prometryn throughout the four seasons sampled. Triazine herbicide concentrations in the Hartbeespoort Dam surface water were highest in summer and gradually decreased in successive seasons of autumn, winter and spring. Terbuthylazine was the only triazine herbicide detected at all sampling sites in the Jukskei River, though atrazine recorded much higher concentrations for the N14 and Kyalami sites, with concentrations of 923 and 210ngL respectively, compared to 134 and 74ngL for terbuthylazine. Analytical results in conjunction with river flow data indicate that the Jukskei and Crocodile Rivers contribute the greatest triazine herbicide loads into the Hartbeespoort Dam. No triazine herbicides were detected in the fish muscle tested, showing that bioaccumulation of triazine herbicides is negligible. Atrazine and terbuthylazine metabolites were detected in the fish muscle with deethylatrazine (DEA) being detected in both catfish and carp muscle at low concentrations of 0.2 and 0.3ngg, respectively. Desethylterbuthylazine (DET) was detected only in catfish at a concentration of 0.3ngg. With atrazine herbicide groundwater concentrations being >130ngL for all seasons and groundwater ∑triazine herbicide concentrations ranging between 527 and 367ngL, triazine compounds in the Hartbeespoort Dam catchment may pose a risk to humans and wildlife in light findings of endocrine and immune disrupting atrazine effects by various researchers.

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Establishment and characterization of atrazine degrading cultures from Nigerian agricultural soil using traditional and Bio-Sep bead enrichment techniques

Cited by 16 publications

References 66 publications

Molecular analysis of atrazine-degrading bacteria and catabolic genes in the water column and sediment of a created wetland in an agricultural/urban watershed

Molecular analysis of atrazine-degrading bacteria and catabolic genes in the water column and sediment of a created wetland in an agricultural/urban watershed

Two decades of warming increases diversity of a potentially lignolytic bacterial community

Seasonal variation of chloro-s-triazines in the Hartbeespoort Dam catchment, South Africa

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