Establishment and characterization of an immortalized human oviductal cell line

Lee, Yin Lau; Lee, Ckf; Xu, Jianbing; Wang, Yanling; Tsao, Sai‐Wah; Yeung, William S.B.

doi:10.1002/mrd.1046

Cited by 67 publications

(42 citation statements)

References 38 publications

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“…The variation in the gene expression of the immortalized oviductal cells could be associated with increasing number of cell passages. In this connection, the telomerase activity of the immortalized cells increases with increasing number of cell passages (13). The observation that exogenous iC3b alone can stimulate embryo development to the greatest extent indicates that the molecule is the actual active embryotrophic molecule.…”

Section: Table II Effects Of C3 and Its Fragments On Mouse Embryo Devmentioning

confidence: 96%

The Embryotrophic Activity of Oviductal Cell-derived Complement C3b and iC3b, a Novel Function of Complement Protein in Reproduction

Lee

et al. 2004

Journal of Biological Chemistry

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The oviduct-derived embryotrophic factor, ETF-3, enhances the development of trophectoderm and the hatching process of treated embryos. Monoclonal anti-ETF-3 antibody that abolishes the embryotrophic activity of ETF-3 recognized a 115-kDa protein from the conditioned medium of immortalized human oviductal cells. Mass spectrometry analysis showed that the protein was complement C3. Western blot analysis using an antibody against C3 confirmed the cross-reactivities between anti-C3 antibody with ETF-3 and anti-ETF-3 antibody with C3 and its derivatives, C3b and iC3b. Both derivatives, but not C3, were embryotrophic. iC3b was most efficient in enhancing the development of blastocysts with larger size and higher hatching rate, consistent with the previous reported embryotrophic activity of ETF-3. Embryos treated with iC3b contained iC3b immunoreactivity. The oviductal epithelium produced C3 as evidenced by the presence of C3 immunoreactivity and mRNA in the human oviduct and cultured oviductal cells. Cyclical changes in the expression of C3 immunoreactivity and mRNA were also found in the mouse oviduct with the highest expression at the estrus stage. Molecules involving in the conversion of C3b to iC3b and binding of iC3b were present in the human oviduct (factor I) and mouse preimplantation embryo (Crry and CR3), respectively. In conclusion, the present data showed that the oviduct produced C3/C3b, which was converted to iC3b to stimulate embryo development.Research is ongoing to optimize the embryo culture condition in human in vitro fertilization and embryo transfer programs. A large proportion of time when embryos are cultured in vitro is when they should be developing in the oviduct in vivo. Oviductal microenvironment is generally accepted to provide the best support to early embryo development. Oviductal cell coculture, the culture of embryo with oviductal cells, has been shown to improve the success rate in prospective randomized control clinical trials (1). In vitro experiments show that human oviductal cell coculture enhances the ability of the embryo at blastocyst stage to escape from its outer shell, zona pellucida, in a process known as hatching. Coculture also reduces the fragmentation rate of human embryos (2, 3) and increases the blastulation rate and total cell count per blastocyst of mouse embryos (4).Despite the success of coculture, it is not commonly used in in vitro fertilization and embryo transfer programs because of the complexity in its implementation as a service. Therefore, sequential culture, the use of different media for culturing embryos at different stages of development, is currently the method of choice for improving the outcome of the human in vitro fertilization and embryo transfer program (5). However, the culture condition in sequential culture systems is still suboptimal as the development of human embryos in these systems can be further improved by supplementation of granulocyte-macrophage colony-stimulating factor (6), a cytokine with peak expression during the preimplantation...

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Section: Table II Effects Of C3 and Its Fragments On Mouse Embryo Devmentioning

confidence: 96%

The Embryotrophic Activity of Oviductal Cell-derived Complement C3b and iC3b, a Novel Function of Complement Protein in Reproduction

Lee

et al. 2004

Journal of Biological Chemistry

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“…OE-E6/E7 cells 22 were maintained in DMEM/F12 medium supplemented with 10% fetal bovine serum (growth medium) in 5% CO 2 at 37°C. Cells were seeded at 500,000 cells per well in 12-well dishes and incubated for 24 hours.…”

Section: Treatment Of Oe-e6/e7 Cell Line With Cotinine and The Nachr␣mentioning

confidence: 99%

Cotinine Exposure Increases Fallopian Tube PROKR1 Expression via Nicotinic AChRα-7

Shaw

Oliver

Lee

et al. 2010

The American Journal of Pathology

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Tubal ectopic pregnancy (EP) is the most common cause of maternal mortality in the first trimester of pregnancy; however, its etiology is uncertain. In EP, embryo retention within the Fallopian tube (FT) is thought to be due to impaired smooth muscle contractility (SMC) and alterations in the tubal microenvironment. Smoking is a major risk factor for EP. FTs from women with EP exhibit altered prokineticin receptor-1 (PROKR1) expression, the receptor for prokineticins (PROK). PROK1 is angiogenic, regulates SMC, and is involved in intrauterine implantation. We hypothesized that smoking predisposes women to EP by altering tubal PROKR1 expression. Sera/FT were collected at hysterectomy (n ‫؍‬ 21). Serum levels of the smoking metabolite, cotinine, were measured by enzyme-linked immunosorbent assay. FTs were analyzed by q-RT-PCR , immunohistochemistry , and Western blotting for expression of PROKR1 and the predicted cotinine receptor, nicotinic acetylcholine receptor ␣-7 (AChR␣؊7). FT explants (n ‫؍‬ 4) and oviductal epithelial cells (cell line OE-E6/E7) were treated with cotinine and an nAChR␣؊7 antagonist. PROKR1 transcription was higher in FTs from smokers (P < 0.01). nAChR␣؊7 expression was demonstrated in FT epithelium. Cotinine treatment of FT explants and OE-E6/E7 cells increased PROKR1 expression (P < 0.05) , which was negated by cotreatment with nAChR␣؊7 antagonist. Smoking targets human FTs via nAChR␣؊7 to increase tubal PROKR1, leading to alterations in the tubal microenvironment that could predispose to EP.

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“…The homology comparisons also showed that there were 40-50% smilarities between rabbit oviductin and some enzymes of chitinase gene family, but the oviductins showed no chitinase activity [14]. In recent years, few researches concerning the roles of oviductins in the development of early embryo were reported [15], [16].…”

Section: Discussionmentioning

confidence: 91%

Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

Pan

et al. 2002

Cell Res

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A full-length rabbit oviductin cDNA(1909bp) was cloned. It consists of a 5'-UTR of 52bp, an open reading frame (ORF) of 1374bp and a 3 -UTR of 483bp and has more than 80% homology with that of other mammal oviductins. N-terminal peptide (NTP) (384 residues) and C-terminal peptide (CTP) (73 residues) of deduced protein precursor has about 80% and 50% identity with that of other mammals respectively. Fusion proteins GST-NTP 368(1R-368N)and GST-CTP73 (369F-441A) were expressed and purified. NH2-terminal of CTP sequencing reveals that the purified protein is consistent with the deduced one. In order to study the function of NTP and CTP the mouse anti-NTP and rabbit anti-CTP antisera were prepared. Tissue-specific (skeleton muscle, oviduct, uterus, ovary, liver, heart and brain) analysis indicated that rabbit oviductin was only found in oviduct. The conditioned medium derived from the rabbit oviduct mucosa epithelial cells has a function of overcoming the early embryonic development block of Kunming mouse cultured in vitro. Anti-CTP antiserum could totally inhibit the early embryo development at 2-cell stage cultured in the conditioned culture medium, but anti-NTP antiserum couldn t. There was a positive relationship between the ratio of early embryos at development block and the dosage of anti-CTP antiserum added in the conditioned culture medium. These results suggest that oviductin has a function not only on fertilization, but also on the release of early embryonic development block, and the later function domain of rabbit oviductin may be situate in its C-terminal.

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Establishment and characterization of an immortalized human oviductal cell line

Cited by 67 publications

References 38 publications

The Embryotrophic Activity of Oviductal Cell-derived Complement C3b and iC3b, a Novel Function of Complement Protein in Reproduction

The Embryotrophic Activity of Oviductal Cell-derived Complement C3b and iC3b, a Novel Function of Complement Protein in Reproduction

Cotinine Exposure Increases Fallopian Tube PROKR1 Expression via Nicotinic AChRα-7

Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

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