Establishment and characterization of a multi-purpose large animal exposure chamber for investigating health effects

Peng, Xinze; Maltz, Mia R.; Botthoff, Jon; Aronson, Emma L.; Nordgren, Tara M.; Lo, David; Cocker, David R.

doi:10.1063/1.5042097

Cited by 7 publications

(9 citation statements)

References 39 publications

(42 reference statements)

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“…Some airborne plant pathogens, such as those causing powdery mildews and sooty molds, may lead to crop losses or forest blights ( Peetz et al, 2007 ; Carisse et al, 2008 ). Previous studies have shown that air pollution, which may be more prevalent at lower elevations, interacts with fungal pathogens to exacerbate asthmatic responses and leads to chronic pulmonary disease or organic toxic dust syndrome in human hosts ( Rask-Andersen and Malmberg, 1990 ; Tunnicliffe et al, 2001 ; Peng et al, 2019 ). Overall, the persistence of these fungal groups likely has important consequences for ecological and human health.…”

Section: Discussionmentioning

confidence: 99%

Landscape Topography and Regional Drought Alters Dust Microbiomes in the Sierra Nevada of California

et al. 2022

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Dust provides an ecologically significant input of nutrients, especially in slowly eroding ecosystems where chemical weathering intensity limits nutrient inputs from underlying bedrock. In addition to nutrient inputs, incoming dust is a vector for dispersing dust-associated microorganisms. While little is known about dust-microbial dispersal, dust deposits may have transformative effects on ecosystems far from where the dust was emitted. Using molecular analyses, we examined spatiotemporal variation in incoming dust microbiomes along an elevational gradient within the Sierra Nevada of California. We sampled throughout two dry seasons and found that dust microbiomes differed by elevation across two summer dry seasons (2014 and 2015), which corresponded to competing droughts in dust source areas. Dust microbial taxa richness decreased with elevation and was inversely proportional to dust heterogeneity. Likewise, dust phosphorus content increased with elevation. At lower elevations, early season dust microbiomes were more diverse than those found later in the year. The relative abundances of microbial groups shifted during the summer dry season. Furthermore, mutualistic fungal diversity increased with elevation, which may have corresponded with the biogeography of their plant hosts. Although dust fungal pathogen diversity was equivalent across elevations, elevation and sampling month interactions for the relative abundance, diversity, and richness of fungal pathogens suggest that these pathogens differed temporally across elevations, with potential implications for humans and wildlife. This study shows that landscape topography and droughts in source locations may alter the composition and diversity of ecologically relevant dust-associated microorganisms.

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Section: Discussionmentioning

confidence: 99%

Landscape Topography and Regional Drought Alters Dust Microbiomes in the Sierra Nevada of California

et al. 2022

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“…Dust samples were collected as described previously. 20 Briefly, airborne particles in regions adjacent to the Salton Sea were collected using passive collectors. These collectors consisted of Teflon-coated round pans (25.4 cm in diameter) filled with quartz marbles that were suspended from the bottom of the pan with Teflon mesh.…”

Section: Methodsmentioning

confidence: 99%

Lung Inflammatory Response to Environmental Dust Exposure in Mice Suggests a Link to Regional Respiratory Disease Risk

Burr

Velazquez

Ulu

et al. 2021

JIR

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“…The aerosol sources for the exposure were each tested in advance in order to determine chemical composition (high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS), Aerodyne) and aerosol density (aerosol particle mass analyzer (APM), Kanomax) to prepare the solutions to yield the targeted particle mass concentration. Sample aerosolization was accomplished by using a homemade nebulizer with silica-gel dryers (Peng et al, 2019). 26 Mice in the control chamber were given filtered dry air (5.0 lpm) only, with other conditions matching the exposure chamber, including bedding replacement, food and water supplies, and corresponding day/night cycle.…”

Section: Chamber Operationmentioning

confidence: 99%

“…Sample aerosolization was accomplished by using a homemade nebulizer with silica-gel dryers (Peng et al, 2019). 26 Mice in the control chamber were given filtered dry air (5.0 lpm) only, with other conditions matching the exposure chamber, including bedding replacement, food and water supplies, and corresponding day/night cycle. Particulate matter was only monitored within the exposure chamber by a scanning mobility particle sizer (SMPS, including Series 3080 Electrostatic Classifier and Ultrafine Condensation Particle Counter 3776, TSI) to assist in maintaining stable PM concentration.…”

Section: Chamber Operationmentioning

confidence: 99%

“…Exposure studies were performed in dual 540 L animal chambers (an exposure chamber and a control chamber) developed as described in Peng et al ( 2019) 26 and as used in Biddle et al (2021). 21 The relative humidity, temperature, and atmospheric pressure were measured in both chambers, with ammonia selectively measured in some exposures to ensure consistent exposure conditions were maintained.…”

Section: Chamber Operationmentioning

confidence: 99%

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Aerosolized Desert Dusts Collected Near a Drying Lake and Pulmonary Inflammation in Mice: Implications for Environmental Exposures and Asthma

Biddle

Yisrael

Drover

et al. 2022

Preprint

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Background: A high incidence of asthma is prevalent among residents near the Salton Sea, a large inland terminal lake in southern California. This arid region has high levels of ambient particulate matter (PM); yet while high PM levels are often associated with asthma in many environments, it is possible that the rapidly retreating lake may contribute components with a specific role in promoting asthma symptoms. Objectives: Our hypothesis is that asthma may be higher in residents closest to the Salton Sea due to chronic exposures to playa dust. Playa emissions may be concentrating dissolved material from the lake, with microbial components capable of inducing pulmonary innate immune responses. Such inflammatory responses may contribute to the development of asthma-like symptoms in residents. To test this hypothesis, we used a mouse model of aerosol exposures to assess the effects of playa dust. Methods: From dust collected around the Salton Sea region, aqueous extracts were used to generate aerosols, which were injected into an environmental chamber for mouse exposure studies. We compared the effects of exposure to Salton Sea aerosols, as well as to known immunostimulatory reference materials. Acute 48-hour and chronic 7-day exposures were compared, with lungs analyzed for inflammatory cell recruitment and gene expression. Results: Dust from sites nearest to the Salton Sea triggered lung neutrophil inflammation that was stronger at 48-hours but reduced at 7-days. This acute inflammatory profile and kinetics resembled the response to innate immune ligands LTA and LPS while distinct from the classic allergic response to Alternaria. Conclusion: Lung inflammatory responses to Salton Sea dusts are similar to acute innate immune responses, raising the possibility that microbial components are entrained in the dust, promoting inflammation. This effect highlights the health risks at drying terminal lakes from inflammatory components in dust emissions from exposed lakebed.

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Establishment and characterization of a multi-purpose large animal exposure chamber for investigating health effects

Abstract: A fusion of principal component analysis and singular value decomposition based multivariate denoising algorithm for free induction decay transversal data Review of Scientific Instruments 90, 035116 (2019);

Cited by 7 publications

References 39 publications

Landscape Topography and Regional Drought Alters Dust Microbiomes in the Sierra Nevada of California

Landscape Topography and Regional Drought Alters Dust Microbiomes in the Sierra Nevada of California

Lung Inflammatory Response to Environmental Dust Exposure in Mice Suggests a Link to Regional Respiratory Disease Risk

Aerosolized Desert Dusts Collected Near a Drying Lake and Pulmonary Inflammation in Mice: Implications for Environmental Exposures and Asthma

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