Establishment and Characterization of a Chinese Hamster Ovary Cell Line, CHO-4J, Stably Expressing a Number of Na+/I−Symporters

Kosugi, Shinji; Sasaki, Norihito; Hai, Noritaka; Sugawa, Hideo; Aoki, Norihiko; Shigemasa, Chiaki; Mori, Toru; Yoshïda, Akio

doi:10.1006/bbrc.1996.1473

Cited by 49 publications

(56 citation statements)

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“…The factors of enhancement of iodide accumulation were 35 -, 41-, and 10 -fold respectively. These values are similar to those achieved by transfection into other mammalian cell lines, 5,8,9 and support the notion that transfection of the NIS gene followed by administration of radioiodine could constitute an approach to the therapy of tumors other than thyroid carcinoma. The kinetics of hNIS-mediated iodide uptake were examined in UVW ± hNIS cells, with V max and K m values obtained using the Lineweaver-Burk double reciprocal plot.…”

Section: Discussionsupporting

confidence: 83%

“…11 However, the observed rates of I À uptake and efflux are similar to those observed in other NIStransfected cell lines, 5,8,9 again implying that I À uptake is solely dependent on NIS expression.…”

Section: Discussionsupporting

confidence: 77%

“…previously published values for NIS -transfected cell lines. 5,8,9 The rate of iodide efflux was also established for UVW ±hNIS cells by incubation of cells with 10 mM NaI containing trace Na 125 I ( specific activity = 100 Ci/ mol ) for 1 hour, followed by replacement of the radioactive incubation buffer with fresh iodide-free buffer. Cells were then incubated at 378C for 3 ±27 minutes, incubation buffer removed, and counted for 125 I À using a gamma counter.…”

Section: Kinetics Of Iodide Uptake and Efflux In Uvw ± Hnis Cellsmentioning

confidence: 99%

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Experimental targeted radioiodide therapy following transfection of the sodium iodide symporter gene: Effect on clonogenicity in both two-and three-dimensional models

et al. 2000

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To evaluate the potential of the expression of the sodium / iodide symporter ( NIS ) as a means of targeting radioiodine to tumor cells, we have employed plasmid -mediated transfection of the NIS gene into a range of mammalian cell hosts. We observed perchlorate -inhibitable iodide uptake up to 41 -fold over control in all NIS -transfected cells. We assessed the effect of NIS expression followed by exposure to 131 I À on the clonogenic survival of UVW glioma cells. After exposure of two -dimensional monolayer cultures of UVW ± NIS cells to 131 I À at a radioactive concentration of 4 MBq / mL, clonogenic survival was reduced to 21%. Similar treatment of UVW ± NIS cells in three -dimensional spheroid cultures resulted in a reduction of clonogenic survival to 2.5%. This increase in sensitivity to 131 I À exposure is likely to be due to a radiological bystander effect. These results are very encouraging for the development of a novel cytotoxic gene -therapy strategy in which a radiological bystander effect plays a significant role in tumor cell sterilization. Cancer Gene Therapy ( 2000 ) 7, 1529 ± 1536

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 77%

Section: Kinetics Of Iodide Uptake and Efflux In Uvw ± Hnis Cellsmentioning

confidence: 99%

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Experimental targeted radioiodide therapy following transfection of the sodium iodide symporter gene: Effect on clonogenicity in both two-and three-dimensional models

et al. 2000

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“…The rapid efflux rate in FRTL-5 cells has been reported (32,42), whereas a low efflux rate was seen when a vector expressing the rat NIS cDNA was transfected into Chinese hamster ovary cells (41,42). In patients with total iodination defects in the thyroid, trapped iodide is completely released within 1 h after administration of ClO 4 -(51).…”

Section: Discussionmentioning

confidence: 99%

“…(c) The subtracted data (net uptake velocity) are expressed as means Ϯ SD (n ϭ 3). (d) The data of 3-300 M NaI, which were chosen according to previous studies (33,36,41), are graphed as Lineweaver-Burk plot. All points of triplicate wells are plotted.…”

Section: Iodide Efflux Is Relatively Low In Mcf-7 Cells Compared Withmentioning

confidence: 99%

Retinoic acid induces sodium/iodide symporter gene expression and radioiodide uptake in the MCF-7 breast cancer cell line

Kogai

Schultz

Johnson

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

127

133

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Molecular and cellular mechanisms of transepithelial iodide transport in the thyroid

Nilsson

1999

BioFactors

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Thyroid hormone is an essential regulator of developmental growth and metabolism in vertebrates. Iodine is a necessary constituent of thyroid hormone. Due to the scarcity and uneven distribution of iodine on the Earth's crust, the structure of the thyroid gland is adjusted to collect and store this element in order to secure a continuous supply of thyroid hormone throughout life. Still, disease resulting from hypothyroidism due to iodine deficiency is a global health problem, illustrating the great biological significance that iodine saving mechanisms have evolved. Iodide is accumulated together with prohormone (thyroglobulin) in the lumen of the thyroid follicles. The rate-limiting step of this transport is the sodium/iodide symporter located in the basolateral plasma membrane of the thyroid follicular cells. Iodide is also transferred across the apical plasma membrane into the lumen where hormonogenesis takes place. In this review, recent progress in the understanding of transepithelial iodide transport in the thyroid is summarized.

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Establishment and Characterization of a Chinese Hamster Ovary Cell Line, CHO-4J, Stably Expressing a Number of Na+/I−Symporters

Cited by 49 publications

References 0 publications

Experimental targeted radioiodide therapy following transfection of the sodium iodide symporter gene: Effect on clonogenicity in both two-and three-dimensional models

Experimental targeted radioiodide therapy following transfection of the sodium iodide symporter gene: Effect on clonogenicity in both two-and three-dimensional models

Retinoic acid induces sodium/iodide symporter gene expression and radioiodide uptake in the MCF-7 breast cancer cell line

Molecular and cellular mechanisms of transepithelial iodide transport in the thyroid

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