Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with the myelodysplastic syndrome

Tohyama, Kaoru; Tsutani, Hiroshi; Ueda, Takanori; Nakamura, Tõru; Yoshida, Yataro

doi:10.1111/j.1365-2141.1994.tb04904.x

Cited by 49 publications

(57 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…18 Interestingly, whereas P39 showed normal patterns of expression of ZNF140 and MNDA, MDS92L demonstrated the low expression seen in adult familial MDS (Figures 5 and 6b).…”

Section: Verification and Extension Of Expression Array Datamentioning

confidence: 90%

“…T cells were isolated from normal peripheral blood and stimulated to proliferate with PHA, followed by IL-2, as described previously, 16 and MNCs were positively isolated using anti-CD4/CD8-conjugated microbeads, according to the manufacturer's instruction (Dynal). The MDS cell lines, P39 17 and MDS92L 18 and the promyelocytic leukemia cell line HL60, 19 were cultured in RPMI containing 10% fetal bovine serum, with the latter requiring IL-3 supplementation.…”

Section: Preparation Of Cell Samplesmentioning

confidence: 99%

“…GAPDH is visible at 18 cycles in unsubtracted cDNA from individual II-6, whereas in subtracted cDNA the GAPDH product is only visible at 23 cycles, giving an estimated 10-fold enrichment. [16][17][18][19] Table 1. (c) Clusters of genes and ESTs 45-fold overexpressed in affected family members, compared with unaffected controls.…”

Section: Differentially Expressed Genes In Adult Mdsmentioning

confidence: 99%

See 2 more Smart Citations

Differentially expressed genes in adult familial myelodysplastic syndromes

et al. 2004

View full text Add to dashboard Cite

The precise genetic events leading to myelodysplastic syndromes (MDSs) and leukemic transformation remain poorly defined. Even less is known about adult familial MDS. We report an adult MDS family in whom enriched tissue-specific transcripts were derived by subtractive hybridization of cDNA from the mononuclear and CD34 þ cells of affected and unaffected family members. These expression libraries were then hybridized to Genome Discovery arrays containing 18 404 genes and expressed sequence tags, and several clusters of differentially expressed genes were identified. A group of 21 genes was underexpressed (45-fold) in affected vs unaffected family members, and among these were transcription factors and genes involved in myeloid differentiation, such as ZNF140 and myeloid nuclear differentiation antigen (MNDA). Another group of 36 genes was overexpressed (45-fold), and these encoded proteins belonging to signaling pathways, such as Ras-and Fos-related genes. The top two genes downregulated in this MDS family, ZNF140 and MNDA, were similarly altered in another MDS family, and in some cases of sporadic MDS. Our data suggest that we have identified genes differentially expressed in adult familial MDS, and that alteration of some of these genes may also be important for the evolution of different stages or severity of sporadic MDS.

show abstract

“…18 Interestingly, whereas P39 showed normal patterns of expression of ZNF140 and MNDA, MDS92L demonstrated the low expression seen in adult familial MDS (Figures 5 and 6b).…”

Section: Verification and Extension Of Expression Array Datamentioning

confidence: 90%

Section: Preparation Of Cell Samplesmentioning

confidence: 99%

Section: Differentially Expressed Genes In Adult Mdsmentioning

confidence: 99%

See 1 more Smart Citation

Differentially expressed genes in adult familial myelodysplastic syndromes

et al. 2004

View full text Add to dashboard Cite

show abstract

“…21,22 MDS-L cells were positive for CD34, c-Kit, HLA-DR, CD13, CD33 and partially positive for CD41 and negative for CD3, CD14, CD20 and glycophorinA. The main karyotype was 49, XY, þ 1, der(5)t(5;19), À7, þ 8, À12, der(13)t(7;13), der(14)t (12;14), der(15)t(15;15), þ 19, þ 20, þ 21, der(22)t (11;22).…”

Section: Cell Lines and Culturementioning

confidence: 99%

Lenalidomide induces cell death in an MDS-derived cell line with deletion of chromosome 5q by inhibition of cytokinesis

et al. 2010

View full text Add to dashboard Cite

Myelodysplastic syndromes (MDS) are a group of hematopoietic stem cell disorders characterized by refractory cytopenias and susceptibility to leukemic transformation. On a subset of MDS patients with deletion of the long arm of chromosome5 (del(5q)), lenalidomide exerts hematological and cytogenetic effects, but the underlying pharmacological mechanisms are not fully understood. In this study, we have investigated the in vitro effects of lenalidomide on an MDS-derived cell line, MDS-L, which carries del(5q) and complex chromosome abnormalities. We found that the growth of MDS-L cells was specifically suppressed mainly by apoptosis, and in addition, multinucleated cells were frequently formed and finally died out in the presence of lenalidomide. Time-lapse microscopic observation and the DNA ploidy analysis revealed that lenalidomide does not affect DNA synthesis but inhibits cytokinesis of MDS-L cells. The gene expression profile showed decreased expression of M phase-related genes such as non-muscle myosin heavy-chain 10, polo-like kinase 1, aurora kinase B, citron kinase and kinesin family member 20A(KIF20A). Interestingly, KIF20A is located at 5q31. These data contribute to the understanding of action mechanisms of lenalidomide on MDS with del(5q) and complex abnormalities.

show abstract

“…Normal peripheral blood cells were obtained from 9 healthy volunteers. Alternatively, we used MDS92 cell line which was established from the bone marrow of an MDS patient (Tohyama et al, 1994). After the sample smears were stained with May-Gruenwald-Giemsa, the cells were morphologically assessed, captured visually with a CCD camera and their coordinates (xand y-) on the slide were recorded (Fig.2a-g, upper stand.)…”

Section: Dna Ploidy Analysis On Megaloblastic Anemia Using Lscmentioning

confidence: 99%