Establishment and Characterization of a Human Gastric Carcinoma Cell Line TMC-1

Abstract

Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61%, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and β-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53. The cell had the deletion at chromosome 18q and gains at chromosome 2p13–25, 5p15, 5q21–35, 7, 8q24, 9q, 11, 12p, 14q24–32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27–32% by interferon-α (2,000 U/ml) and by interferon-γ with an IC₅₀ of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-α and interferon-γ. It can be used to develop new modalities of human gastric cancer treatment.

Quantitative Proteomic and Genomic Profiling Reveals Metastasis-Related Protein Expression Patterns in Gastric Cancer Cells

“…SC-M1 is a well-established human gastric carcinoma cell line, originally cultured from a poorly differentiated adenocarcinoma that invaded through the stomach wall but showed no metastasis to lymph nodes or adjacent organs . Another gastric cancer cell line, TMC-1, was derived from the lymph node of an individual with a moderately differentiated adenocarcinoma of the stomach and exhibited highly tumorigenic features . The comparative mRNA and protein profiling of these two cell lines may allow us to study the cellular processes associated with the development of gastric cancer metastasis, facilitating further investigation by clinical specimens.…”

“…20 Another gastric cancer cell line, TMC-1, was derived from the lymph node of an individual with a moderately differentiated adenocarcinoma of the stomach and exhibited highly tumorigenic features. 21 The comparative mRNA and protein profiling of these two cell lines may allow us to study the cellular processes associated with the development of gastric cancer metastasis, facilitating further investigation by clinical specimens.…”