ESTABLISHMENT AND CHARACTERIZATION OF A NEW HUMAN TESTICULAR GERM CELL TUMOR CELL LINE (TCam-2)

Mizuno, Yohei; Gotoh, Akinobu; Kamidono, Sadao; Kitazawa, Sohei

doi:10.5980/jpnjurol1989.84.1211

Cited by 88 publications

(113 citation statements)

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“…These were GCT27, GCT44, Tera1, Tera2, H12.2, 2102Ep, 577MF, NTERA2, SUSA and TCam-2. The cell lines were cultured as described previously (Pera et al, 1987;Kelland et al, 1992;Mizuno et al, 1993;Summersgill et al, 2001;Goddard et al, 2007). A pool of 45 individual samples of normal testis RNA was available from Clontech (Mountain View, CA, USA) and normal DNA from Sigma (St Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Genomic copy number and expression patterns in testicular germ cell tumours

McIntyre

Summersgill

et al. 2007

Br J Cancer

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Testicular germ cell tumours of adults and adolescents (TGCT) include seminomas (SE) and nonseminomas (NS), with spermatocytic seminomas (SSE) representing a distinct entity in older men. SE and NS have gain of 12p material in all cases, whereas SSE are associated with overrepresentation of chromosome 9. Here, we compare at the chromosomal level, copy number imbalances with global expression changes, identified by comparative expressed sequence hybridisation analyses, in seven SE, one combined tumour, seven NS and seven cell lines. Positive correlations were found consistent with copy number as a main driver of expression change, despite reported differences in methylation status in SE and NS. Analysis of chromosomal copy number and expression data could not distinguish between SE and NS, in-keeping with a similar genetic pathogenesis. However, increased expression from 4q22, 5q23.2 and 9p21 distinguished SSE from SE and NS and decreased copy number and expression from 2q36 -q37 and 6q24 was a specific feature of NS-derived cell lines. Our analysis also highlights 19 regions with both copy number and expression imbalances in greater than 40% of cases. Mining available expression array data identified genes from these regions as candidates for involvement in TGCT development. Supplementary data is available at

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Section: Methodsmentioning

confidence: 99%

Genomic copy number and expression patterns in testicular germ cell tumours

McIntyre

Summersgill

et al. 2007

Br J Cancer

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“…18 The human TCam-2 cell line was primarily derived from a testicular tumor sample of pure classical seminoma in 1992. 19 In this study, TCam-2 cells (kindly gifted from Dr. Riko Kitazawa, Department of Diagnostic Pathology, Ehime University Hospital, Matsuyama, Japan) were maintained in complete medium (Roswell Park Memorial Institute Medium-1640 with 10% fetal calf serum) in a humidified 5% CO 2 , 37 C incubator.…”

Section: Chemical Compounds and Antibodiesmentioning

confidence: 99%

TDRG1 regulates chemosensitivity of seminoma TCam-2 cells to cisplatin via PI3K/Akt/mTOR signaling pathway and mitochondria-mediated apoptotic pathway

Gan

Wang

Tan

et al. 2016

Cancer Biology & Therapy

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“…However, with the exception of primary tumor samples, few tools are available to study the molecular pathogenesis of seminoma (15). To the best of our knowledge and according to the literature, only 3 cell lines (TCam-2, JKT-1 and SEM-1) originate from seminoma (16)(17)(18). Furthermore, the origin of these cell lines is not certain; therefore it remains controversial to describe these cell lines as seminoma cell lines (19,20).…”

Section: Discussionmentioning

confidence: 99%

In vitro study on shRNA-mediated reduction of testis developmental related gene 1 expression and its effects on the proliferation, invasion and apoptosis of NTERA-2 cells

et al. 2015

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Abstract. Testis developmental related gene 1 (TDRG1) is a novel human testis-specific gene. TDRG1 is differentially expressed in cancerous tissue compared with normal testicular tissue and demonstrates a unique expression pattern in normal testes; therefore, this gene may be involved in the occurrence and development of testicular germ cell tumors (TGCT). In the present study, the expression level of TDRG1 was downregulated in human TGCT NTERA-2 cells by RNA interference (RNAi) in order to investigate the association between TDRG1 and TGCT. The TDRG1 mRNA and protein expression levels in NTERA-2 cells were significantly inhibited following transfection with specific RNAi plasmids. The ability to proliferate (inhibited by 15.4% at day 3 and 26.1% at day 5; P<0.001) and invade (reduced by 49.1%; P=0.01) in vitro was suppressed in cells in which the expression level of TDRG1 was reduced, and a corresponding increase in the apoptotic potential was observed (the early apoptotic potential and total apoptotic potential were increased by 75%; P=0.019 and 54.8%; P=0.009, respectively). The results of the present study indicated that the biological behavior of NTERA-2 cells is associated with TDRG1 expression levels, and that this gene may be a novel target candidate in the treatment of TGCT.

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ESTABLISHMENT AND CHARACTERIZATION OF A NEW HUMAN TESTICULAR GERM CELL TUMOR CELL LINE (TCam-2)

Cited by 88 publications

References 0 publications

Genomic copy number and expression patterns in testicular germ cell tumours

Genomic copy number and expression patterns in testicular germ cell tumours

TDRG1 regulates chemosensitivity of seminoma TCam-2 cells to cisplatin via PI3K/Akt/mTOR signaling pathway and mitochondria-mediated apoptotic pathway

In vitro study on shRNA-mediated reduction of testis developmental related gene 1 expression and its effects on the proliferation, invasion and apoptosis of NTERA-2 cells

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