Establishment and Characterization of a Malignant Epithelioid Hemangioendothelioma from Mouse Tnyroid Tumor.

Hosoda, Chihiro; Ohtsuru, Akira; Namba, Hiroyuki; Motomura, Katsuaki; Hara, Takeshi; Nakashima, Masahiro; Ito, Masahiro; Yokoro, Kenjiro; Yamashita, Shunichi

doi:10.1507/endocrj.42.341

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…Cell Culture-The mouse hemangioendothelioma (MHE) cell line established from the thyroid, expressing factor VIII and vimetidine (19), was used as vascular endothelial cells. MHE cells can incorporate acetylated low-density lipoprotein and stain positively for factor VIII antigen.…”

Section: Methodsmentioning

confidence: 99%

Long Exposure to High Glucose Concentration Impairs the Responsive Expression of γ-Glutamylcysteine Synthetase by Interleukin-1β and Tumor Necrosis Factor-α in Mouse Endothelial Cells

Urata

Yamamoto

Goto

et al. 1996

Journal of Biological Chemistry

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134

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To elucidate the pathological metabolism of glutathione synthesis in diabetic endothelial cells, we studied the expression of gamma-glutamylcysteine synthetase (gamma-GCS) using a mouse vascular endothelial cell line. Exposing normoglycemic endothelial cells to tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta) increased the activity and the mRNA expression of gamma-GCS. The addition of inhibitors for nuclear factor kappaB (NF-kappaB) to the cells caused a loss of the gamma-GCS mRNA expression in response to TNF-alpha. A shift of the concentration of glucose in the medium from 5.5 to 28 mM glucose and a following incubation for 7 days decreased the expression of gamma-GCS mRNA. These cells showed no apparent responses of gamma-GCS mRNA or the activity of NF-kappaB to TNF-alpha or IL-beta. Increase in the GSH concentration of the cells treated with 28 mM glucose restored the expression of gamma-GCS mRNA and its response to TNF-alpha or IL-beta, suggesting that redox regulation is involved in the expression of gamma-GCS. In summary, the expression of gamma-GCS is regulated by TNF-alpha or IL-1beta in endothelial cells mediated by NF-kappaB stimulation, and impairment of the regulation of gamma-GCS in hyperglycemic cells may be a cause of medical complications that develop in diabetes mellitus.

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Section: Methodsmentioning

confidence: 99%

Long Exposure to High Glucose Concentration Impairs the Responsive Expression of γ-Glutamylcysteine Synthetase by Interleukin-1β and Tumor Necrosis Factor-α in Mouse Endothelial Cells

Urata

Yamamoto

Goto

et al. 1996

Journal of Biological Chemistry

Self Cite

134

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“…Finally, concerning the molecular pathogenesis of primary thyroidal angiosarcomas, Hosoda et al (28) have recently reported on the establishment and characterization of a malignant angiosarcoma-like cell line, mEHE/CH5, which was derived from a mouse thyroid tumor and also overexpressed the proto-oncogene c-myc. Interestingly, the growth of these cells were thyrotropin (TSH)-independent, but could be inhibited by cAMP and vitamin D 3 (28).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the growth of these cells were thyrotropin (TSH)-independent, but could be inhibited by cAMP and vitamin D 3 (28).…”

Section: Discussionmentioning

confidence: 99%

Juxtathyroidal Neck Soft Tissue Angiosarcoma Presenting as an Undifferentiated Thyroid Carcinoma

Steiner

Muench

et al. 2002

Thyroid

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Angiosarcoma is a malignant growth of endothelial origin, uncommon in the head and neck. We present the case of a 38-year-old woman with long-standing goiter who presented with a rapidly growing 6.0-cm neck mass. Fine-needle aspiration biopsy of the tumor showed features of "undifferentiated thyroid carcinoma (ThyrCa)." Total thyroidectomy resulted in extirpation of all gross disease. Pathology revealed a high-grade angiosarcoma of the neck invading the thyroid gland, coexisting with papillary ThyrCa (follicular variant) in the contralateral lobe. Aggressive external electron beam radiotherapy was initiated for local control. Despite the absence of systemic dissemination initially, bulky neck recurrences, and pulmonary metastases developed rapidly, leading to the patient's demise on postoperative day 41. Autopsy showed metastatic disease involving most organs. This case illustrates that neck angiosarcomas need to be considered in the differential diagnosis of "poorly differentiated" thyroid malignancies. These soft tissue neck tumors may complicate postoperative management due to their bleeding tendency and aggressive infiltrative behavior, and carry a dismal prognosis because of the rapidity of development of local recurrence and distant metastases.

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Establishment of a new murine-phenotypic angiosarcoma cell line (ISOS-1)

Masuzawa

Fujimura

Tsubokawa

et al. 1998

Journal of Dermatological Science

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Establishment and Characterization of a Malignant Epithelioid Hemangioendothelioma from Mouse Tnyroid Tumor.

Abstract: Abstract. A mouse epithelioid hemangioendothelioma (mEHE) cell line, passagable in vivo without TSH stimulation, was established from a thyroid tumor.

Cited by 3 publications

References 26 publications

Long Exposure to High Glucose Concentration Impairs the Responsive Expression of γ-Glutamylcysteine Synthetase by Interleukin-1β and Tumor Necrosis Factor-α in Mouse Endothelial Cells

Long Exposure to High Glucose Concentration Impairs the Responsive Expression of γ-Glutamylcysteine Synthetase by Interleukin-1β and Tumor Necrosis Factor-α in Mouse Endothelial Cells

Juxtathyroidal Neck Soft Tissue Angiosarcoma Presenting as an Undifferentiated Thyroid Carcinoma

Establishment of a new murine-phenotypic angiosarcoma cell line (ISOS-1)

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