Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1

Liebertz, Daniel J.; Lechner, Melissa G.; Masood, Rizwan; Sinha, Uttam K.; Han, Jing; Puri, Raj K.; Correa, Adrian; Epstein, Alan L.

doi:10.1186/1758-3284-2-5

Cited by 39 publications

(49 citation statements)

References 20 publications

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“…We generated a xenograft metastasis model where USC-HN1 cells, highly metastatic human SCC cells (4), were labeled with GFP for tracking and injected to immunocompromised NOD.Cg mice. After 5 weeks, as visualized by GFP, the injected hSCC cells formed primary SCC tumors (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…SCC-15 and USC-HN1 (previously described and charactrerized in (4)) cell lines were cultured in DMEM High Glucose (Gibco) supplemented with 10% FBS (HyClone), 100IU penicillin and 100μg/mL streptomycin (Gibco). MDA-MB-231 cells were cultured in RPMI-1640 (Gibco) supplemented with 10% FBS (HyClone), 1mM sodium pyruvate (Gibco), 100IU penicillin and 100μg/mL streptomycin (Gibco).…”

Section: Methodsmentioning

confidence: 99%

“…Head and neck squamous cell carcinoma (HNSCC) is highly aggressive and account for 45,000 malignancies diagnosed each year and is the fifth leading cancer worldwide (3–6). Despite various treatment options available, HNSCC patients are still faced with a high chance of recurrence and/or metastasis, which is responsible for the poor clinical prognosis, with a 5-year survival rate of only about 50 percent (4, 5). …”

Section: Introductionmentioning

confidence: 99%

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α-Catulin Marks the Invasion Front of Squamous Cell Carcinoma and Is Important for Tumor Cell Metastasis

Cao

Chen

Masood

et al. 2012

Molecular Cancer Research

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Squamous cell cancers comprise the most common types of human epithelial cancers. One subtype, head and neck squamous cell carcinoma (HNSCC), is a particularly aggressive cancer with poor prognosis due to late diagnosis and lymph node metastasis. Of all the processes involved in carcinogenesis, local invasion and distant metastasis are clinically the most relevant, but are the least well understood on a molecular level. Here we find that in vivo, the α-catenin homologue – α-catulin, a protein originally reported to interact with Lbc Rho guanine nucleotide exchange factor, is highly expressed at the tumor invasion front and in the metastatic streams of cells in both malignant human HNSCCs and a mouse model of oral SCC. Knockdown of α-catulin in hHNSCC cell lines dramatically decrease the migratory and invasive potential of those cells in vitro and metastatic potential in xenotransplants in vivo. Analysis of tumors deficient in α-catulin showed that the tumor cells are unable to invade the surrounding stroma. Accordingly, transcriptional profiling of those tumors revealed that α-catulin ablation is accompanied by changes in genes involved in cell migration and invasion. Interestingly enough, in vitro experiments show that an upregulation of α-catulin expression correlates with the transition of tumor cells from an epithelial to a mesenchymal morphology, as well as an upregulation of EMT markers vimentin and snail. Overall, these results strongly indicate that α-catulin contributes to the invasive behavior of metastatic cells, and may be used as a prognostic marker and future therapeutic target for cancer patients.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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α-Catulin Marks the Invasion Front of Squamous Cell Carcinoma and Is Important for Tumor Cell Metastasis

Cao

Chen

Masood

et al. 2012

Molecular Cancer Research

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“…For instance, RPeL27 is upregulated in cells/tissues of sulforaphane treated-breast cancers [10], gastric tubular adenoma and carcinoma [11], hepatocellular carcinoma (HCC) [12], and metastatic liver lesions [13], while RPeL43 is upregulated in cases of astrocytomas [14], head and neck squamous cell carcinoma [15, 16], and hepatitis B-associated HCC [17]. The RPeL41 gene has been found to be overexpressed in neoplastic lung fibroblast cells [18].…”

Section: Discussionmentioning

confidence: 99%

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

Sim

Chan

et al. 2016

Disease Markers

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Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes' involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (RPeL27, RPeL41, and RPeL43) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support RPeL27, RPeL41, and RPeL43 as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins.

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“…Tumor cell lines were obtained from ATCC or gifted to the Epstein laboratory and authenticity was verified by cytogenetics and surface marker analysis as described previously 18 . HNSCC tumor biopsy samples were obtained and used under USC Keck School of Medicine IRB-approved protocol HS-09-00048.…”

Section: Methodsmentioning

confidence: 99%

USC-HN2, a new model cell line for recurrent oral cavity squamous cell carcinoma with immunosuppressive characteristics

Russell¹,

Lechner²,

Gong³

et al. 2011

Oral Oncology

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Objectives Head and neck squamous cell carcinomas (HNSCC) are common and aggressive tumors that have not seen an improvement in survival rates in decades. These tumors are believed to evade the immune system through a variety of mechanisms and are therefore highly immune modulatory. In order to elucidate their interaction with the immune system and develop new therapies targeting immune escape, new pre-clinical models are needed. Materials and Methods A novel human cell line, USC-HN2, was established from a patient biopsy specimen of invasive, recurrent buccal HNSCC and characterized by morphology, heterotransplantation, cytogenetics, phenotype, gene expression and immune modulation studies and compared to a similar HNSCC cell line; SCCL-MT1. Results and Conclusion Characterization studies confirmed the HNSCC origin of USC-HN2 and demonstrated a phenotype similar to the original tumor and typical of aggressive oral cavity HNSCC (EGFR+CD44v6+FABP5+Keratin+ and HPV−). Gene and protein expression studies revealed USC-HN2 to have highly immune-modulatory cytokine production (IL-1β, IL-6, IL-8, GM-CSF, and VEGF) and strong regulatory T and myeloid derived suppressor cell (MDSC) induction capacity in vitro. Of note, both USC-HN2 and SCCL-MT1 were found to have a more robust cytokine profile and MDSC induction capacity when compared to 7 previously established HNSCC cell lines. Additionally, microarray gene expression profiling of both cell lines demonstrate up-regulation of antigen presenting genes. Because USC-HN2 is therefore highly immunogenic, it also induces strong immune suppression to evade immunologic destruction. Based upon these results, both cell lines provide an excellent model for the development of new suppressor cell-targeted immunotherapies.

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Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1

Cited by 39 publications

References 20 publications

α-Catulin Marks the Invasion Front of Squamous Cell Carcinoma and Is Important for Tumor Cell Metastasis

α-Catulin Marks the Invasion Front of Squamous Cell Carcinoma and Is Important for Tumor Cell Metastasis

Human Ribosomal Proteins RPeL27, RPeL43, and RPeL41 Are Upregulated in Nasopharyngeal Carcinoma Cell Lines

USC-HN2, a new model cell line for recurrent oral cavity squamous cell carcinoma with immunosuppressive characteristics

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