Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection

Song, Yingying; Shou, Shuyu; Guo, Huimin; Gao, Zixiang; Liu, Nannan; Yang, Yang; Wang, Feifei; Deng, Qiang; Liu, Jing; Xie, Youhua

doi:10.1016/j.virs.2022.05.002

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…Considering the practical disadvantages of HepG2-NTCP cells, the HepG2-NTCP-A3/C2 subclone has relatively increased viral production under PEG-free conditions and showed an improved in vitro HBV infection system [145]. Additionally, hepatocyte nuclear factor 4α and the NTCPexpressing hepatoma cell line, BEL7404, have been permissive for HBV replication and susceptible to HBV infection [146]. Nevertheless, more cell culture models with increased susceptibility to HBV infection need to be identified and studied.…”

Section: Nctp-expressed Hepatoma Derived Hepg2 Cell Linesmentioning

confidence: 99%

Cell Culture Systems for Studying Hepatitis B and Hepatitis D Virus Infections

Lee

Purdy

Choi

2023

Life

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The hepatitis B virus (HBV) and hepatitis D virus (HDV) infections cause liver disease, including hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). HBV infection remains a major global health problem. In 2019, 296 million people were living with chronic hepatitis B and about 5% of them were co-infected with HDV. In vitro cell culture systems are instrumental in the development of therapeutic targets. Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. Current HBV therapeutics, such as nucleoside analogs, effectively suppress viral replication but are not curative. Additionally, no effective treatment for HDV infection is currently available. Therefore, there is an urgent need to develop therapies to treat both viral infections. A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. However, the lack of an efficient cell culture system hampers the development of novel antiviral strategies for HBV/HDV infections. In vitro cell culture models have evolved with significant improvements over several decades. Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. We address various cell culture systems along with different cell lines and how these cell culture systems can be used to provide better tools for HBV and HDV studies.

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Section: Nctp-expressed Hepatoma Derived Hepg2 Cell Linesmentioning

confidence: 99%