Establishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line HKESC-4 of Chinese origin

Cheung, Leo C. M.; Tang, Johnny; Lee, P. Y.; Hu, Liang; Guan, Xin Yuan; Tang, Wing Ka; Srivastava, Gopesh; Wong, John W.; Luk, John M.; Law, Simon

doi:10.1016/j.cancergencyto.2007.05.026

Cited by 28 publications

(13 citation statements)

References 26 publications

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“…These cell lines were previously established by our team [16–20]. NE-1 cells were cultured in keratinocyte-SFM medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (Invitrogen, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

Cytoplasmic Forkhead Box M1 (FoxM1) in Esophageal Squamous Cell Carcinoma Significantly Correlates with Pathological Disease Stage

et al. 2011

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Esophageal cancer is a deadly cancer with esophageal squamous cell carcinoma (ESCC) as the major type. Until now there has been a lack of reliable prognostic markers for this malignancy. This study aims to investigate the clinical correlation between Forkhead box M1 (FoxM1) and patients’ parameters in ESCC.MethodsImmunohistochemistry was performed to investigate the expression and localization of FoxM1 in 64 ESCC tissues and 10 nontumor esophageal tissues randomly selected from 64 patients before these data were used for clinical correlations.ResultsCytoplasmic and nuclear expressions of FoxM1 were found in 63 and 16 of the 64 ESCC tissues, respectively. Low cytoplasmic expression of FoxM1 was correlated with early pathological stage in ESCC (P = 0.018), while patients with nuclear FoxM1 were younger in age than those without nuclear expression (P < 0.001). Upregulation of FoxM1 mRNA was found in five ESCC cell lines (HKESC-1, HKESC-2, HKESC-3, HKESC-4, and SLMT-1) when compared to non-neoplastic esophageal squamous cell line NE-1 using quantitative polymerase chain reaction (qPCR). Except for HKESC-3, all studied ESCC cell lines demonstrated a high expression of FoxM1 protein using immunoblot. A high mRNA level of FoxM1 was observed in all of the ESCC tissues examined when compared to their adjacent nontumor tissues using qPCR.ConclusionCytoplasmic FoxM1 was correlated with pathological stage and might be a biomarker for advanced ESCC.

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Section: Methodsmentioning

confidence: 99%

Cytoplasmic Forkhead Box M1 (FoxM1) in Esophageal Squamous Cell Carcinoma Significantly Correlates with Pathological Disease Stage

et al. 2011

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“…Karyotypic analysis showed that the ESC‐410 ESCC cell line, established in this experiment, contained a mixed population of hyperdiploid and polyploid cells, which indicated the phenotypic and genotypic heterogeneity of this cell line 16 …”

Section: Discussionmentioning

confidence: 65%

“…Cells with variable epithelial morphological features were observed and grew adherently in a monolayer as described in a former study 7 . Ultrastructural examinations revealed tonofilament bundles in the cytoplasm and numerous desmosomes in the intercellular connections, which are the characteristic features of epithelial cells 7,16 …”

Section: Discussionmentioning

confidence: 78%

“…In this experimental study, a new ESCC cell line, ESC‐410, was established from the ESCC tissue derived from a 65‐year‐old male patient with gastric cardiac and cervical lymph node metastases. Until now, this cell line was maintained for more than 16 months and passaged for more than 60 times, and kept growth potential well; the population doubling time was about 53 h, shorter than that reported previously, 63 h for the Hong Kong esophageal squamous cell (HKESC‐4) cell line 16 …”

Section: Discussionmentioning

confidence: 89%

“…Until now, this cell line was maintained for more than 16 months and passaged for more than 60 times, and kept growth potential well; the population doubling time was about 53 h, shorter than that reported previously, 63 h for the Hong Kong esophageal squamous cell (HKESC-4) cell line. 16 Cells with variable epithelial morphological features were observed and grew adherently in a monolayer as described in a former study. 7 Ultrastructural examinations revealed tonofilament bundles in the cytoplasm and numerous desmosomes in the intercellular connections, which are the characteristic features of epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of a novel human esophageal squamous cell carcinoma cell line (ESC-410) and its partial biological characterization

Zhang

Gao

et al. 2010

Diseases of the Esophagus

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Esophageal cancer exhibits an uneven geographical distribution strikingly, resulting in focal endemic high-incidence areas in several countries worldwide including China, which might be associated with the environmental and genetic risk factors in those areas. Permanent cancer cell lines are invaluable tools in understanding the biology of cancers and experimental therapeutics. To enrich cell line panel and animal models of human esophageal squamous cell carcinoma (ESCC) from different geographical areas and investigate the environmental and genetic risk factors in the carcinogenesis of ESCC, a novel human esophageal squamous cancer cell line (ESC-410) was established. The cell line grew adherent as a monolayer and maintained stable growth rate with a doubling time of 53 h and distinct epithelial morphological appearance; it was maintained in vitro for 18 months and subcultured for more than 50 passages. Ultrastructural examination revealed large irregular nuclei, desmosome, and tonofilaments; karyotype analysis showed a modal number of chromosomes that ranged from 35 to 73, with a median of 57, and 77% of analyzed cells were hyperdiploidy; reverse transcription polymerase chain reaction (RT-PCR) detected the mRNA expressions of CK8, CK18, and CK19 in the established cells; immunofluorescence assay identified the protein expressions of neurotrophin receptor p75 and integrin α6 (CD49f) in the ESC-410 cell line; xenotransplantation of ESC-410 cells into athymic nude mice subcutaneously induced the formation of solid tumor masses in about 2 weeks. By histopathological examination, heterogeneity of xenograft tumor was observed, as same as that of human primary ESCC. All findings and evidence in this experimental study suggested that this cell line might be a useful model in vitro and in vivo in cellular and molecular studies as well as in testing novel therapies for human ESCC.

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Prognostic significance of phosphorylated RON in esophageal squamous cell carcinoma

et al. 2011

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Esophageal squamous cell carcinoma (ESCC) is the most common type of esophageal cancer. RON is a transmembrane receptor overexpressed in various cancers; however, the clinical significance of its phosphorylated form (pRON) is not fully deciphered. This report is the first to investigate the expression and clinical significance of pRON in human ESCC. Quantitative polymerase chain reaction revealed an up-regulation of RON mRNA in 70% (7/10) of ESCC tissues when compared to the adjacent nontumor tissues. An overexpression of pRON protein was found in most of the ESCC cell lines studied (4/5) when compared to two non-neoplastic esophageal epithelial cells using immunoblot. In 64 ESCC tissues, pRON was localized at the cell membrane, cytoplasm and nucleus in 15 (23.4%), 63 (98.4%) and 61 (95.3%) cases using immunohistochemistry. Patients having high expression of cytoplasmic pRON significantly associated with shorter median survival when compared to those with low expression (25.41 months vs. 14.43 months), suggesting cytoplasmic pRON as a potential marker for poor prognosis in ESCC patients.

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Establishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line HKESC-4 of Chinese origin

Cited by 28 publications

References 26 publications

Cytoplasmic Forkhead Box M1 (FoxM1) in Esophageal Squamous Cell Carcinoma Significantly Correlates with Pathological Disease Stage

Cytoplasmic Forkhead Box M1 (FoxM1) in Esophageal Squamous Cell Carcinoma Significantly Correlates with Pathological Disease Stage

Establishment of a novel human esophageal squamous cell carcinoma cell line (ESC-410) and its partial biological characterization

Prognostic significance of phosphorylated RON in esophageal squamous cell carcinoma

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