Establishment and characterization of a carcinoembryonic antigen producing cell line derived from human pancreatic exocrine cancer

Yamaguchi, Naohito; Uozumi, Gentsu; Ikeuchi, Hideo; Morioka, Hiroyuki; Machino, Mitsuo; Kawai, Keiichi

doi:10.1007/bf02774699

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…The origin, histological type, grade of differentiation and mutational status of the most frequent genetic changes (p53, K-ras, DPC4/smad4, p16) of PDCL investigated are reported in Table 1 [11,12,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38]. All the cell lines were cultured as monolayers in RPMI 1640 (Biochrom, Berlin, Germany) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Hyclone, Logan, UT), penicillin and streptomycin (100 mg/ml) under standard culture conditions (5% CO 2 , 95% air in humidified chamber at 37C).…”

Section: Cell Linesmentioning

confidence: 99%

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A comprehensive in vitro characterization of pancreatic ductal carcinoma cell line biological behavior and its correlation with the structural and genetic profile

et al. 2004

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There are a large number of stable pancreatic ductal carcinoma cell lines (PDCL) that are used by researchers worldwide. Detailed data about their differentiation status and genetic alterations are present in the literature, but a systematic correlation with cell biological behavior is often lacking. PDCL ( n=12) were clustered by source of tumor cell (ascites, primary tumor, metastasis), and the data of functional cell biology were correlated with the reported structural and genetic profiles. Major histocompatibility complex expression, chemosensitivity and aneuploidia appeared to be related to the source of PDCL, and proliferative capacity appeared to be related to the grade of differentiation. No correlation between genetic/structural features of PDCL and biological behavior was found. All the cell lines appeared generally insensitive to in vitro treatment with 5-fluorouracil and showed variable degrees of susceptibility to gemcitabine, raltitrexed and oxaliplatin. All the PDCL showed resistance to Fas-mediated apoptosis but were significantly sensitive to the pro-apoptotic effect of inflammatory cytokines [interleukin (IL)-1beta, tumor necrosis factor (TNF)alpha and interferon gamma]. PDCL were characterized for the secretion of several factors relevant to the tumor-immune cross talk. Vascular endothelial growth factor, CCL2, CCL5 and transforming growth factor beta were the factors most frequently released; less frequent was the secretion of CXCL8, CCL22, IL-6 and sporadically CXCL12, IL-10 and hepatocyte growth factor. The cytokines IL-1beta and TNFalpha were always undetectable. In conclusion, a clear correlation between structural/genetic features and function could not be detected, suggesting the weakness of a "morphological" classification for the in vitro studies of pancreatic cancer.

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Section: Cell Linesmentioning

confidence: 99%

“…Mut [23,30] ? Mut [31] T3M-4 T. Okabe (Japan) [32,33] Male, 64 PDAC G2 [11,12,24,34] Mut (p.m) [11,12] Mut (meth) [11,12] W.t. [11,12] AsPC-1 Ascites M.H Tan (USA) [35,36] Female, 62 PDAC G2 G2 10 26 Yes G1/G2/G3 [35,36] ATCC Mut (p.m.) [11,12] Mut (f.s.)…”

Section: Pdac G1mentioning

confidence: 99%

A comprehensive in vitro characterization of pancreatic ductal carcinoma cell line biological behavior and its correlation with the structural and genetic profile

et al. 2004

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“…The guide RNA (gRNA) target sequence (5ʹ-CATGCGCTGTGACCGCAAGA-3ʹ) was designed using the online gRNA design tool crispr.mit.edu and subcloned into the plasmid LentiCRISPRV2 (52961, Addgene, Cambridge, MA). The generation of lentiviral particles has been previously described (Yamaguchi et al 1983). QGP1 cells were infected with lentiviral particles containing either LentiCRISPRV2 empty-vector control or gRNAcontaining LentiCRISPRV2 in medium supplemented with 8 µg/mL polybrene (Sigma-Aldrich).…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats (C...mentioning

confidence: 99%

“…The PanNET cell line QGP1 is an immortalized human cell line that was established in 1980 from a somatostatinproducing islet cell carcinoma (Yamaguchi et al 1983).…”

Section: Creation Of a Men1 Knockout Cell Line In Qgp1 Cells Using Cr...mentioning

confidence: 99%

Loss of MEN1 function impairs DNA repair capability of pancreatic neuroendocrine tumors

Lakiza

Lutze

Vogle

et al. 2022

Endocrine-Related Cancer

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Somatic MEN1 mutations occur in up to 50% of pancreatic neuroendocrine tumors (PanNETs). Clinical studies have shown that radiation therapy (IR) is effective in a subset of PanNETs, but it remains unclear why some patients respond better to IR than others. Herein, we study whether MEN1 loss of function increases radiosensitivity of PanNETs and determine its effect on DNA double strand break (DSB) repair. After creating a MEN1 knockout PanNET cell line, we confirmed reduced DSB repair capacity in MEN1 deficient cells and linked these findings to a defect in homologous recombination, as well as reduced BRCA2 expression levels. Consistent with this model, we found that MEN1 mutant cells displayed increased sensitivity to the highly trapping poly (ADP-ribose) polymerase (PARP) 1 inhibitor talazoparib in vitro. Our results suggest that combining IR with PARP inhibition may be beneficial in patients with PanNETs and MEN1 loss of function.

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Establishment of a human carcinoembryonic antigen (CEA)-producing cell line in a protein-free chemically defined medium

Yamaguchi

Okabe

Kawai

1985

J Cancer Res Clin Oncol

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To examine whether a human carcinoembryonic antigen (CEA)-producing cell can proliferate and sythesize CEA in vitro in culture without protein supplements, long-term cultivation of such cells was carried out in a protein-free chemically defined medium. Using stepwise decreases in fetal bovine serum concentration, continuous growth of the cells was established in a protein-free am's F-12 medium. The cells, designated as HLC-Yl, have been propagated in this medium for 3 years. The population doubling time of the cells is about 52 h. Addition of the serum stimulated the cell growth (population doubling time = 27 h). Saturation density was not increased by the addition of serum. The cells grown in a protein-free F-12 secrete large amounts of CEA (65.4 +/- 2.6 ng/10(6) cells/24 h). Addition of serum did not stimulate the production of CEA. The cells produced tumours when inoculated into athymic nude mice. The mice bearing the tumour showed high serum CEA levels, and CEA was demonstrated in the tumour tissue by the immunoperoxidase method. The present study suggests that cells grown in a protein-free medium do not require serum components for their growth or CEA synthesis and provide an excellent model for better understanding the growth and production of CEA in human lung cancer cells.

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Establishment and characterization of a carcinoembryonic antigen producing cell line derived from human pancreatic exocrine cancer

Cited by 7 publications

References 15 publications

A comprehensive in vitro characterization of pancreatic ductal carcinoma cell line biological behavior and its correlation with the structural and genetic profile

A comprehensive in vitro characterization of pancreatic ductal carcinoma cell line biological behavior and its correlation with the structural and genetic profile

Loss of MEN1 function impairs DNA repair capability of pancreatic neuroendocrine tumors

Establishment of a human carcinoembryonic antigen (CEA)-producing cell line in a protein-free chemically defined medium

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