Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient

Abstract

A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured <i>in Vitro</i>

“…Saponaro et al [19] demonstrate that the VEGF-A expression in BRCA1-2 carrier breast cancer tissues was higher than that in BRCAX breast cancer tissues. This confirms what we have in AMJ13 the cell line under investigation which is BRCA1 positive [12]. Furthermore, in both BRCA1-2 carriers and BRCAX the VEGF-A expression was higher than the sporadic group.…”

“…Cancer cells secret many factors that activate angiogenesis process to support themselves for nutrients and waste removal [3]. Our experiment is designed to study newly established breast cancer cell line named AMJ13 [12] in regards to its ability to induce angiogenesis by screening its secretions during cells propagations in vitro. This cell line is important as it is first Iraqi breast cancer model and we need to study cancer cells angiogenesis factors and to know which main factor this cancer cells depend on during angiogenesis.…”

“…Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. The cells were positive for BRCA1 and BRCA2, as well as for vimentin [12].…”

Cytotoxic Activity of Iraqi Cressa Cretica

“…0.0021** 0.0009** 0.0004** 0.0001** 0.0001** 0.0001** (10). Figure (11) showed the presence of different chemical constituents with major peak at Rt 3.45minutes representing percent area of 80.2%of the total compositions as illustrated in Table (5).…”

Selective cytotoxic effect of Plantago lanceolata L. against breast cancer cells

“…The human breast cancer cell lines AMJ13 [20], MCF7, MDAMB, and CAL51, as well as mouse embryo fibroblasts (MEF) were supplied by Cell Bank Unit. AMJ13 cells were cultured in RPMI-1640 medium (USbiological, USA) supplemented with 10% fetal bovine serum (FBS) (Capricorn Scientific, Germany), 100 units/mL penicillin, and 100 μg/ mL streptomycin.…”