Establishment and characterization of a cell line (OMC-9) originating from a human endometrial stromal sarcoma

Abstract

Cell lines are very useful for clinical and basic research. The establishment of uterine malignant tumor cell lines with unusual histology is especially important. We describe the establishment and characterization of a new human endometrial stromal sarcoma cell line of the uterus. The cell line OMC-9 was established from a tumor mass in the uterine body of a 55-year-old woman. Characteristics of the cell line studied include morphology, chromosome analysis, heterotransplantation, tumor markers and chemosensitivity. This cell line has grown well for 196 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, appear to be spindle-shaped or multipolar and have a tendency to pile up without contact inhibition. The cells exhibit a human karyotype with a modal chromosomal number in the diploid range. The cells were able to be transplanted into the subcutis of nude mice and produced tumors resembling the original tumor. OMC-9 cells produced tissue polypeptide antigen. Both CD10, a sensitive and diagnostically useful marker of endometrial stromal neoplasms, and vimentin were identified immunohistochemically in the original tumor and the heterotransplanted tumor. The cells were sensitive to actinomycin D, doxorubicin, carboplatin, cisplatin and etoposide, drugs used commonly in the treatment of gynecologic cancer. Only three reports of uterine endometrial stromal sarcoma cell lines have thus far been reported in the literature. OMC-9 is the first endometrial stromal sarcoma cell line in which CD10 expression and chemosensitivity have been identified.

Identification of a novel, recurrent MBTD1‐CXorf67 fusion in low‐grade endometrial stromal sarcoma

“…cDNAs prepared from OMC‐9 ESS cell line and from GIST‐T1 cell line were used as negative controls (for more details, see Supporting Information).…”

Salvage cytoreductive surgery for pleural dissemination of low-grade endometrial stromal sarcoma using rib-cross thoracotomy with argon beam coagulation: strategies to achieve a grossly complete resection—a case report

“…Moreover, LG-ESS is classified into 3 categories based on molecular genetic abnormalities as follows: translocation t(7;17) (p15;q21); 6p21rearrangements; and X;22 or 7-rearrangements (11). Cell lines for genetic and molecular biological investigations have been developed (12). As LG-ESS is basically hormone sensitive, hormone therapy with MPA or letrozole, in addition to chemotherapy, can contribute to the improvement of prognosis in patients with residual disease, progressive disease, or recurrence (3)(4)(5)(6)(7).…”

248 Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo

“…For some other cell lines details regarding tumorigenicity in nude mice are missing. In a recent publication Kakuno et al reported the establishment of a new cell line (OMC-9) originated from a human endometrial stromal sarcoma [4]. According to the authors, these cells are tumorigenic in nude mice and could, therefore, be useful for development of an in vivo system.…”