Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma

Abstract

In vitro models are required for the study of these cancers, and several cell lines have already been established and characterised (Lafargues & Ozzello, 1958;Soule et al., 1973;Cailleau et al., 1974;Engel et al., 1978;Whitehead et al., 1983;Yamane et al., 1984;Chu et al., 1985;Vandewalle et al., 1987). Nevertheless, owing to the heterogenity and the diversity of mammary cancers, a great number of cell models is necessary to understand the reasons for this diversity and the effect of anticancer drugs on tumour cells.Cytogenetic studies of mammary adenocarcinoma cell lines are essential for comprehension of the pathogenesis of these cancers (Trent, 1985;Gebhart et al., 1986). The implication of chromosomal alterations in these pathologies has opened a new and promising route towards better knowledge of these cancers (Cervenka & Koulischer, 1973). Chromosomal alterations are generally numerous, and markers often demonstrate hyperploidy in these cancers (Sandberg, 1980). Demonstration of the minimum genetic alterations indispensable for cell transformation is difficult, and might be easier on cells with a karyotype closer to normal. Sandberg and Wolman mentioned the existence of such cells, but most of their results concerned karyotype studies without chromosome banding (Sandberg, 1980;Wolman, 1983 [-', hyaluronidase 25 IU for 20 ml, Hepes buffer 4.8 g 1' in distilled water). Cells were then fixed in acetic acid:methanol (1:3) and dropped onto grease-free, cooled slides for chromosome counting and examination. R bands were obtained by heat denaturation of the chromosomes according to the method of Dutrillaux and Lejeune (1971). Xenografted CAL51 cells were plated and studied in vitro in the same manner.

Molecular cytogenetic analysis of breast cancer cell lines

“…CAL51 was perfectly diploid, as previously reported by classical banding (Gioanni et al, 1990). MT-3 showed minimal deviation from diploidy -iso13 q, trisomy 7 and loss of X.…”

BRK tyrosine kinase expression in a high proportion of human breast carcinomas

“…MCF 10A (ATCC-CRL 10317) were grown in DMEM Hams F12, 5% horse serum, hydrocortisone (0.5 mg/ml), cholera toxin (0.1 mg/ml), insulin (5 mg/ml) and EGF (0.01 mg/ml). (Whitehead et al, 1983) and Cal51 (Gioanni et al, 1990) were routinely cultured in DMEM, 10% FBS.…”

Chfr inactivation is not associated to chromosomal instability in colon cancers

“…The chfr-specific probe (623 pb) was generated by PCR using 5 0 -AGT CCA GGA TTA CGT GTG CCC-3 0 (sense) and 5 0 -CCT CCA GTG CTG CTG AAA GCT GCT C-3 0 (antisense) primers mutation leading to MIN (Wheeler et al, 1999;Gayet et al, 2001). All breast cancer cell lines were aneuploid with the exception of HME-1 and Cal-51 (Gioanni et al, 1990;Forozan et al, 2000;Kytola et al, 2000;Rummukainen et al, 2001).…”