Establishment and biochemical characterization of tamarillo (Solanum betaceum Cav.) embryogenic cell suspension cultures

Alves, Ana; Caeiro, André; Correia, Sandra; Verı́ssimo, Paula; Canhoto, Jorge

doi:10.1007/s11627-017-9864-z

Cited by 10 publications

(9 citation statements)

References 55 publications

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“…The integration of fractionation and/or type-enrichment for proteins or peptides, including resolving protein homogenate by 1D-PAGE followed by in-gel digestion and LC-MS/MS antibody-based acetylated-lysine enrichment ( Zhao et al, 2015a , b ; dos Santos et al, 2016 ; Xia et al, 2016 ; Alves et al, 2017 ), may provide new insights and in-depth proteome coverage during the induction of SE. However, few of the exquisite variety of proteomic tools have been implemented so far.…”

Section: Plant Proteomics Technologiesmentioning

confidence: 99%

“…Biochemical analyses of the proteolytic activity reveal that serine proteases, aspartic proteases, and metalloproteases are dominant in EC, whereas serine proteases dominate NEC in Solanum betaceum ( Alves et al, 2017 ). Leucine aminopeptidase has been found to be unique to PEG-dependent somatic embryo maturation of C. papaya ( Vale et al, 2014 ).…”

Section: Proteolytic Enzymes and The Ubiquitin Proteasome Systemmentioning

confidence: 99%

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Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Aguilar-Hernández

Loyola‐Vargas

2018

Front. Plant Sci.

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Somatic embryogenesis (SE) is a cell differentiation process by which a somatic cell changes its genetic program and develops into an embryonic cell. Investigating this process with various explant sources in vitro has allowed us to trace somatic embryo development from germination to plantlets and has led to the generation of new technologies, including genetic transformation, endangered species conservation, and synthetic seed production. A transcriptome data comparison from different stages of the developing somatic embryo has revealed a complex network controlling the somatic cell’s fate, suggesting that an interconnected network acts at the protein level. Here, we discuss the current progress on SE using proteomic-based data, focusing on changing patterns of proteins during the establishment of the somatic embryo. Despite the advanced proteomic approaches available so far, deciphering how the somatic embryo is induced is still in its infancy. The new proteomics techniques that lead to the quantification of proteins with different abundances during the induction of SE are opening this area of study for the first time. These quantitative differences can elucidate the different pathways involved in SE induction. We envisage that the application of these proteomic technologies can be pivotal to identifying proteins critical to the process of SE, demonstrating the cellular localization, posttranslational modifications, and turnover protein events required to switch from a somatic cell to a somatic embryo cell and providing new insights into the molecular mechanisms underlying SE. This work will help to develop biotechnological strategies for mass production of quality crop material.

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Section: Plant Proteomics Technologiesmentioning

confidence: 99%

Section: Proteolytic Enzymes and The Ubiquitin Proteasome Systemmentioning

confidence: 99%

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Aguilar-Hernández

Loyola‐Vargas

2018

Front. Plant Sci.

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“…The second group of substrates ( Figure 5 d–f) was used to evaluate the presence of glycoside hydrolases (MU-G, MU-NAG, and MU-C) and alkaline phosphatases (MU-P). The choice of these substrates is based on previous works with tamarillo cell suspension cultures [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of ICL cellular protein profiles revealed high expression levels of stress-related proteins [ 11 , 12 ], confirming the role of tissue culture systems in inducing molecular reprogramming. During recent experiments, S. betaceum ICL was grown efficiently as PCSs [ 13 ]. The evaluation of their extracellular protein patterns showed that cell suspensions of tamarillo produce and secrete a wide array of proteins into the culture medium, including endoglucanases, proteases, and low molecular weight (>20 kDa) peptides [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…During recent experiments, S. betaceum ICL was grown efficiently as PCSs [ 13 ]. The evaluation of their extracellular protein patterns showed that cell suspensions of tamarillo produce and secrete a wide array of proteins into the culture medium, including endoglucanases, proteases, and low molecular weight (>20 kDa) peptides [ 13 ]. These results followed the previously obtained intracellular protein profiles of tamarillo ICL [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the Production of Hydrolytic Enzymes in Elicited Tamarillo (Solanum betaceum Cav.) Cell Suspension Cultures

Casimiro

Mota

Verı́ssimo

et al. 2023

Plants

Self Cite

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Plant cell suspension cultures are widely used as a tool for analyzing cellular and molecular processes, metabolite synthesis, and differentiation, bypassing the structural complexity of plants. Within the range of approaches used to increase the production of metabolites by plant cells, one of the most recurrent is applying elicitors capable of stimulating metabolic pathways related to defense mechanisms. Previous proteomics analysis of tamarillo cell lines and cell suspension cultures have been used to further characterize and optimize the growth and stress-related metabolite production under in vitro controlled conditions. The main objective of this work was to develop a novel plant-based bioreactor system to produce hydrolytic enzymes using an elicitation approach. Based on effective protocols for tamarillo micropropagation and plant cell suspension culture establishment from induced callus lines, cell growth has been optimized, and enzymatic activity profiles under in vitro controlled conditions characterized. By testing different sucrose concentrations and the effects of two types of biotic elicitors, it was found that 3% (w/v) sucrose concentration in the liquid medium enhanced the production of hydrolytic enzymes. Moreover, casein hydrolysate at 0.5 and 1.5 g/L promoted protein production, whereas yeast extract (0.5 g/L) enhanced glycosidase activity. Meanwhile, chitosan (0.05 and 0.1 g/L) enhanced glycosidases, alkaline phosphates, and protease activities.

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Analysis of the potential involvement of lncRNAs in embryogenic competence of Solanum betaceum Cav. (tamarillo) calli

Cordeiro,

Camelo,

Pedrosa

et al. 2024

Plant Cell Tiss Organ Cult

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Somatic embryogenesis (SE) is a process by which somatic cells reprogram, acquire totipotency and embark on embryo formation. Although SE is a valuable tool for micropropagation in many crops, it presents specific challenges in woody species due to some bottlenecks, such as loss of embryogenic competence during subcultures and the often-low somatic embryo conversion into plantlets. Hence, great interest exists in exploring the regulatory networks involved on SE. As transcription modulators, long non-coding RNAs (lncRNAs) have been found essential in a wide range of biological processes. This work aimed to identify lncRNAs related to the embryogenic competence in Solanum betaceum Cav. (tamarillo). Nanopore® long-read sequencing was conducted in cell lines with distinct cell fates and, based on their coding potential, 60 transcripts were selected as lncRNA candidates. Similar expression patterns were found among embryogenic cell lines (EC) and cells that lost their embryogenic potential (herein long-term callus, LTC), whereas non-EC (NEC) showed differentially expressed lncRNAs. Whereas lncRNAs upregulated in EC and LTC were predicted to target embryogenesis-related genes, such as AGAMOUS-like 15 and WUSCHEL-related HOMEOBOX 2, lncRNAs upregulated in NEC were predicted to target mainly genes involved in carbohydrate metabolism, cell wall formation, auxin and ethylene signalling pathways. Altogether, these results show the involvement of lncRNA in the process of somatic embryogenesis in S. betaceum, indicating that EC present a pattern of lncRNA expression that suggests its action on genes that directly modulate the morphogenic response in vitro, while in NEC the modulation through this type of RNAs seems to be predominantly reflected in genes more related to cellular physiology. Graphical Abstract

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Establishment and biochemical characterization of tamarillo (Solanum betaceum Cav.) embryogenic cell suspension cultures

Cited by 10 publications

References 55 publications

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Enhancing the Production of Hydrolytic Enzymes in Elicited Tamarillo (Solanum betaceum Cav.) Cell Suspension Cultures

Analysis of the potential involvement of lncRNAs in embryogenic competence of Solanum betaceum Cav. (tamarillo) calli

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