Establishing the pangolin mitochondrial D-loop sequences from the confiscated scales

Hsieh, Hsing-Mei; Lee, James Chun-I; Wu, Jane-Hong; Chen, Chao‐An; Chen, Yen-Jean; Wang, Guan‐Bang; Chin, Shih-Chien; Wang, Lih-Chiann; Linacre, Adrian; Tsai, Li-Chin

doi:10.1016/j.fsigen.2010.06.003

Cited by 35 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among those, 44 "Manis sp." attributed to scales seized in Taiwan (Hsieh et al, 2011) actually belonged to M. javanica ( Fig. 2 and Supplementary Table 2).…”

Section: To the Editormentioning

confidence: 99%

What's behind these scales? Comments to “The complete mitochondrial genome of Temminck's ground pangolin (Smutsia temminckii; Smuts, 1832) and phylogenetic position of the Pholidota (Weber, 1904)”

Gaubert

Antunes

2015

Gene

View full text Add to dashboard Cite

“…Among those, 44 "Manis sp." attributed to scales seized in Taiwan (Hsieh et al, 2011) actually belonged to M. javanica ( Fig. 2 and Supplementary Table 2).…”

Section: To the Editormentioning

confidence: 99%

What's behind these scales? Comments to “The complete mitochondrial genome of Temminck's ground pangolin (Smutsia temminckii; Smuts, 1832) and phylogenetic position of the Pholidota (Weber, 1904)”

Gaubert

Antunes

2015

Gene

View full text Add to dashboard Cite

“…Pangolins (monotypic order Pholidota and family Manidae) are believed to be the most heavily poached and trafficked mammals in the world, accounting for as much as 20% of all illegal transnational wildlife trade [1][2][3][4]. Overexploitation driven by escalating demand for their meat as luxury food consumption, and scales for the traditional medicines has driven pangolins to the edge of extinction [5][6][7][8][9][10]. More than one million pangolins have been poached in the decade prior to 2014 [11].…”

Section: Introductionmentioning

confidence: 99%

Genomic consequences of population decline in critically endangered pangolins and their demographic histories

Hao

Frantz

et al. 2020

National Science Review

110

View full text Add to dashboard Cite

Pangolins are among the most critically endangered animals due to heavy poaching and world wild trafficking. However, their demographic histories and the genomic consequences of their recent population declines remain unknown. We generated high-quality de novo reference genomes for critically endangered Malayan (Manis javanica, MJ) and Chinese (M. pentadactyla, MP) pangolins and re-sequencing population genomic data from 74 MJs and 23 MPs. We recovered the population identities of illegally traded pangolins and previously unrecognized genetic populations that should be protected as evolutionarily distinct conservation units. Demographic reconstruction suggested environmental changes have resulted in population size fluctuation of pangolins. Additionally, recent population size declines due to human activities have resulted in an increase in inbreeding and genetic load.Deleterious mutations were enriched in genes related to cancer/diseases and cholesterol homeostasis, which may have increased their susceptibility to diseases and decreased their survival potential to adapt to environmental changes and high-cholesterol diet. This comprehensive study provides not only high-quality pangolin reference genomes, but also valuable information concerning the driving factors of long-term population size fluctuations and the genomic impact of recent population size declines due to human activities, which is essential for pangolin conservation management and global action planning.

show abstract

“…The traceability of the pangolin trade is rendered difficult by the various forms under which pangolins are traded, including live animals, smoked carcasses, chopped meat, scales, paws and embryos. Despite the availability of molecular markersnotably mitochondrial DNAto trace the pangolin trade (Gaubert & Antunes 2015), only a few studies focusing on Asian species have so far applied the genetic tool to the survey of pangolin seizures (Luo et al 2009;Hsieh et al 2011;Zhang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Phylogeography of the heavily poached African common pangolin (Pholidota, Manis tricuspis) reveals six cryptic lineages as traceable signatures of Pleistocene diversification

et al. 2016

View full text Add to dashboard Cite

Knowledge on faunal diversification in African rainforests remains scarce. We used phylogeography to assess (i) the role of Pleistocene climatic oscillations in the diversification of the African common pangolin (Manis tricuspis) and (ii) the utility of our multilocus approach for taxonomic delineation and trade tracing of this heavily poached species. We sequenced 101 individuals for two mitochondrial DNA (mtDNA), two nuclear DNA and one Y-borne gene fragments (totalizing 2602 bp). We used a time-calibrated, Bayesian inference phylogenetic framework and conducted character-based, genetic and phylogenetic delineation of species hypotheses within African common pangolins. We identified six geographic lineages partitioned into western Africa, Ghana, the Dahomey Gap, western central Africa, Gabon and central Africa, all diverging during the Middle to Late Pleistocene. MtDNA (cytochrome b + control region) was the sole locus to provide diagnostic characters for each of the six lineages. Tree-based Bayesian delimitation methods using single- and multilocus approaches gave high support for 'species' level recognition of the six African common pangolin lineages. Although the diversification of African common pangolins occurred during Pleistocene cyclical glaciations, causative correlation with traditional rainforest refugia and riverine barriers in Africa was not straightforward. We conclude on the existence of six cryptic lineages within African common pangolins, which might be of major relevance for future conservation strategies. The high discriminative power of the mtDNA markers used in this study should allow an efficient molecular tracing of the regional origin of African common pangolin seizures.

show abstract

Establishing the pangolin mitochondrial D-loop sequences from the confiscated scales

Cited by 35 publications

References 31 publications

What's behind these scales? Comments to “The complete mitochondrial genome of Temminck's ground pangolin (Smutsia temminckii; Smuts, 1832) and phylogenetic position of the Pholidota (Weber, 1904)”

What's behind these scales? Comments to “The complete mitochondrial genome of Temminck's ground pangolin (Smutsia temminckii; Smuts, 1832) and phylogenetic position of the Pholidota (Weber, 1904)”

Genomic consequences of population decline in critically endangered pangolins and their demographic histories

Phylogeography of the heavily poached African common pangolin (Pholidota, Manis tricuspis) reveals six cryptic lineages as traceable signatures of Pleistocene diversification

Contact Info

Product

Resources

About