Establishing the adipose stem cell identity: Characterization assays and functional properties

Harrison, Mark A.; Al-Ghadban, Sara; O’Donnell, Benjamen T.; Mohiuddin, Omair A.; Wise, Rachel M.; Sullivan, Brianne N.; Bunnell, Bruce A.

doi:10.1016/b978-0-12-819376-1.00002-0

Cited by 4 publications

(2 citation statements)

References 222 publications

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Section: Discussionmentioning

confidence: 99%

“…However, later research demonstrated that AT is indeed an endocrine organ secreting factors involved in several biological and regulatory processes and also contains other cell types including preadipocytes, pericytes, fibroblasts, endothelial cells, hematopoietic cells, macrophages, and ASCs ( Gimble et al, 2014 ). ASCs can be obtained through enzymatic digestion of LA samples, isolated from SVF by plastic adherence and culture expanded in vitro ( Fraser et al, 2007 ; Lee et al, 2020 ; Harrison et al, 2022 ). Interestingly, ASCs isolated from different subcutaneous fat depots (i.e., abdomen, gluteal region, thigh) show different marker expression and multi-differentiation potential, suggesting that ASCs harvested from different anatomical niches may present unique properties ( Ong et al, 2014 ; Tsuji, 2014 ; Di Taranto et al, 2015 ; Vadala et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

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Micro-fragmented and nanofat adipose tissue derivatives: In vitro qualitative and quantitative analysis

Cicione

Vadalà

Giacomo

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Adipose tissue is widely exploited in regenerative medicine thanks to its trophic properties, mainly based on the presence of adipose-derived stromal cells. Numerous devices have been developed to promote its clinical use, leading to the introduction of one-step surgical procedures to obtain minimally manipulated adipose tissue derivatives. However, only a few studies compared their biological properties. This study aimed to characterize micro-fragmented (MAT) and nanofat adipose tissue (NAT) obtained with two different techniques.Methods: MAT, NAT and unprocessed lipoaspirate were collected from surgical specimens. RNA extraction and collagenase isolation of stromal vascular fraction (SVF) were performed. Tissue sections were analysed by histological and immunohistochemical (collagen type I, CD31, CD34 and PCNA) staining to assess tissue morphology and cell content. qPCR was performed to evaluate the expression of stemness-related (SOX2, NANOG and OCT3/4), extracellular matrix (COL1A1) and inflammatory genes (IL1β, IL6 and iNOS). Furthermore, multilineage differentiation was assessed following culture in adipogenic and osteogenic media and staining with Oil Red O and Alizarin red. ASC immunophenotype was assessed by flow cytometric analysis of CD90, CD105, CD73 and CD45.Results: Histological and immunohistochemical results showed an increased amount of stroma and a reduction of adipocytes in MAT and NAT, with the latter displaying the highest content of collagen type I, CD31, CD34 and PCNA. From LA to MAT and NAT, an increasing expression of NANOG, SOX2, OCT3/4, COL1A1 and IL6 was noted, while no significant differences in terms of IL1β and iNOS emerged. No statistically significant differences were noted between NAT and SVF in terms of stemness-related genes, while the latter demonstrated a significantly higher expression of stress-related markers. SVF cells derived from all three samples (LA, MAT, and NAT) showed a similar ASC immunoprofile as well as osteogenic and adipogenic differentiation.Discussion: Our results showed that both MAT and NAT techniques allowed the rapid isolation of ASC-rich grafts with a high anabolic and proliferative potential. However, NAT showed the highest levels of extracellular matrix content, replicating cells, and stemness gene expression. These results may provide precious clues for the use of adipose tissue derivatives in the clinical setting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Micro-fragmented and nanofat adipose tissue derivatives: In vitro qualitative and quantitative analysis

Cicione

Vadalà

Giacomo

et al. 2023

Front. Bioeng. Biotechnol.

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Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications

Ahmed,

Tauseef,

Mohiuddin

2024

Methods in Molecular Biology

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Adipose Stem Cells in Regenerative Medicine: Looking Forward

Al-Ghadban

Artiles

Bunnell

2022

Front. Bioeng. Biotechnol.

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Over the last decade, stem cell-based regenerative medicine has progressed to clinical testing and therapeutic applications. The applications range from infusions of autologous and allogeneic stem cells to stem cell-derived products. Adult stem cells from adipose tissue (ASCs) show significant promise in treating autoimmune and neurodegenerative diseases, vascular and metabolic diseases, bone and cartilage regeneration and wound defects. The regenerative capabilities of ASCs in vivo are primarily orchestrated by their secretome of paracrine factors and cell-matrix interactions. More recent developments are focused on creating more complex structures such as 3D organoids, tissue elements and eventually fully functional tissues and organs to replace or repair diseased or damaged tissues. The current and future applications for ASCs in regenerative medicine are discussed here.

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Establishing the adipose stem cell identity: Characterization assays and functional properties

Cited by 4 publications

References 222 publications

Micro-fragmented and nanofat adipose tissue derivatives: In vitro qualitative and quantitative analysis

Micro-fragmented and nanofat adipose tissue derivatives: In vitro qualitative and quantitative analysis

Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications

Adipose Stem Cells in Regenerative Medicine: Looking Forward

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