Establishing EGFP Congenic Mice in a NOD/Shi-scid IL2Rgnull (NOG) Genetic Background Using a Marker-Assisted Selection Protocol (MASP)

Suemizu, Hiroshi; Yagihashi, Chie; Mizushima, Tomoko; Ogura, Tomoyuki; Etoh, Tomoo; Kawai, Kenji; Ito, Mamoru

doi:10.1538/expanim.57.471

Cited by 13 publications

(16 citation statements)

References 11 publications

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“…The murine line used in the present study was assigned the genetic designation NOG- Tg (Pgk-EGFP) 16 /ShiJic (formally, NOD.Cg- Prkdc scid Il2rg tm1Sug Tg (Pgk-EGFP) 16/ ShiJic, abridged name: PgkEGFP-NOG). We also generated BALB/cA.Cg- Tg (Pgk-EGFP) 16 /Jic (abridged name, PgkEGFP-BALB) and C57BL/6J.Cg- Tg (Pgk-EGFP) 16 /Jic (abridged name, PgkEGFP-C57BL/6) by crossing the inbred PgkEGFP-NOG strain with the BALB/cA or C57BL/6J strain, respectively, using the speed congenic method [18]. This study was performed in accordance with the guidelines of our institution and was approved by the Animal Experimentation Committee of the Central Institute for Experimental Animals.…”

Section: Methodsmentioning

confidence: 99%

“…NOG mice have no lymphocytes or NK cells and have impaired dendritic cells and macrophage function [9, 13]. Additionally, we previously reported an EGFP-expressing NOG mouse line (NOG-EGFP mouse) [18]. Since the NOG-EGFP mouse demonstrates severe immunodeficiency and systemic EGFP expression, it is considered the most appropriate recipient mouse for analyzing the microenvironment of xenograft tissues [15].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues

et al. 2014

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The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues

et al. 2014

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“…We generated NOG IL-3/GM-Tg mice by speed congenic techniques using a marker-assisted selection protocol (36) to change the genetic background of the mice from C57BL/6J to NOD. IL-3/ GM-Tg mice, in which all of the microsatellite markers were replaced by the NOD haplotype at N6, were further crossed onto the NOG background.…”

Section: Generation Of Nog Il-3/gm-tg Micementioning

confidence: 99%

Establishment of a Human Allergy Model Using Human IL-3/GM-CSF–Transgenic NOG Mice

Ito

Takahashi

Katano

et al. 2013

The Journal of Immunology

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133

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The development of animal models that mimic human allergic responses is crucial to study the pathophysiology of disease and to generate new therapeutic methodologies. Humanized mice reconstituted with human immune systems are essential to study human immune reactions in vivo and are expected to be useful for studying human allergies. However, application of this technology to the study of human allergies has been limited, largely because of the poor development of human myeloid cells, especially granulocytes and mast cells, which are responsible for mediating allergic diseases, in conventional humanized mice. In this study, we developed a novel transgenic (Tg) strain, NOD/Shi-scid-IL2rγnull (NOG), bearing human IL-3 and GM-CSF genes (NOG IL-3/GM–Tg). In this strain, a large number of human myeloid cells of various lineages developed after transplantation of human CD34+ hematopoietic stem cells. Notably, mature basophils and mast cells expressing FcεRI were markedly increased. These humanized NOG IL-3/GM–Tg mice developed passive cutaneous anaphylaxis reactions when administered anti–4-hydroxy-3-nitrophenylacetyl IgE Abs and 4-hydroxy-3-nitrophenylacetyl. More importantly, a combination of serum from Japanese cedar pollinosis patients and cedar pollen extract also elicited strong passive cutaneous anaphylaxis responses in mice. Thus, to our knowledge, our NOG IL-3/GM–Tg mice are the first humanized mouse model to enable the study of human allergic responses in vivo and are excellent tools for preclinical studies of allergic diseases.

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“…NOD/Shi-scid IL2Rg(null) (NOG) mice were injected with single-cell suspension of spheres after partial bone marrow ablation (primary BMT) [6]. At 12-week post-transplantation, peripheral blood, bone marrow-derived cells and splenocytes of xenotransplanted mice were analyzed for human and mouse hematopoietic cell chimerism.…”

Section: Dear Editormentioning

confidence: 99%

Human peripheral blood-born hematosphere as a niche for hematopoietic stem cell expansion

et al. 2011

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Establishing EGFP Congenic Mice in a NOD/Shi-scid IL2Rgnull (NOG) Genetic Background Using a Marker-Assisted Selection Protocol (MASP)

Abstract: Severely immunocompromised NOD/Shi-scid IL2Rgnull (NOG)

Cited by 13 publications

References 11 publications

A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues

A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues

Establishment of a Human Allergy Model Using Human IL-3/GM-CSF–Transgenic NOG Mice

Human peripheral blood-born hematosphere as a niche for hematopoietic stem cell expansion

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