Establishing Criteria for Human Mesenchymal Stem Cell Potency

Samsonraj, Rebekah M.; Rai, Bina; Sathiyanathan, Padmapriya; Puan, Kia Joo; Rötzschke, Olaf; Hui, James; Raghunath, Michael; Stanton, Lawrence W.; Nurcombe, Victor; Cool, Simon M.

doi:10.1002/stem.1982

Cited by 175 publications

(168 citation statements)

References 58 publications

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“…In contrast, PDGFRA was highly expressed in MSC but not informative in perivascular cells, and PDGFRB was highly expressed in both populations. Others have shown that high expression of PDGFRA is associated with highly proliferative MSC colonies, suggesting that its expression is associated with expansion in culture (Samsonraj et al, 2015). These data are consistent with a classification hierarchy determined by mouse and human lineage studies, where multipotent adult cells are quiescent in a perivascular location (Crisan et al, 2008;Acar et al, 2015).…”

Section: Comparison Of Msc and Adult Stem/progenitor Cell Typessupporting

confidence: 78%

A Molecular Classification of Human Mesenchymal Stromal Cells

Rohart

Mason

Matigian

et al. 2015

Preprint

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Mesenchymal stromal cells (MSC) are widely used for the study of mesenchymal tissue repair, and increasingly adopted for cell therapy, despite the lack of consensus on the identity of these cells. In part this is due to the lack of specificity of MSC markers. Distinguishing MSC from other stromal cells such as fibroblasts is particularly difficult using standard analysis of surface proteins, and there is an urgent need for improved classification approaches. Transcriptome profiling is commonly used to describe and compare different cell types; however, efforts to identify specific markers of rare cellular subsets may be confounded by the small sample sizes of most studies. Consequently, it is difficult to derive reproducible, and therefore useful markers. We addressed the question of MSC classification with a large integrative analysis of many public MSC datasets. We derived a sparse classifier (The Rohart MSC test) that accurately distinguished MSC from non-MSC samples with >97% accuracy on an internal training set of 635 samples from 41 studies derived on 10 different microarray platforms. The classifier was validated on an external test set of 1,291 samples from 65 studies derived on 15 different platforms, with >95% accuracy. The genes that contribute to the MSC classifier formed a protein-interaction network that included known MSC markers. Further evidence of the relevance of this new MSC panel came from the high number of Mendelian disorders associated with mutations in more than 65% of the network. These result in mesenchymal defects, particularly impacting on skeletal growth and function. The Rohart MSC test is a simple in silico test that accurately discriminates MSC from fibroblasts, other adult stem/progenitor cell types or differentiated stromal cells.

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Section: Comparison Of Msc and Adult Stem/progenitor Cell Typessupporting

confidence: 78%

A Molecular Classification of Human Mesenchymal Stromal Cells

Rohart

Mason

Matigian

et al. 2015

Preprint

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“…They introduced surface markers including STRO-1 and PDGFR-α as markers for differentiating the MSCs with high and low capacity for ectopic bone formation. They also concluded that MSCs with greater colony-forming efficacy, predominant small-size cells, and greater growth capacity are more potent to form ectopic bone (Samsonraj et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic regulation of specific transcription factors in osteogenicdifferentiation of mesenchymal stem cells

Heydarabad¹,

Vatanmakanian²,

Nikasa³

et al. 2016

Turk J Biol

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An abundance of experiments have been performed to clarify the differentiation process of mesenchymal stem cells (MSCs). Osteogenic differentiation and in vitro conditions favoring these cell lineages have attracted the attention of many researchers. Moreover, the gene expression profile during MSC differentiation toward its main specialized cells (bone, cartilage, and adipose cells) has been mostly understood. In the last decades another layer of investigation has attempted to clarify the epigenetic mechanisms underlying MSC differentiation into its specialized cells. It has been shown that as MSCs progress through the differentiation process, more nonspecific genes undergo DNA methylation to prevent differentiation into improper cell fates. Moreover, promoters of lineage-specific genes are strongly hypomethylated in MSCs during differentiation. The main objective of this review is to address the role of major epigenetic mechanisms such as DNA methylation, histone modifications, and noncoding RNAs, especially miRNAs, in osteoblastic differentiation of MSCs and to summarize all the previous studies that have determined the epigenetic alterations of the nuclear genome during osteoblastic differentiation of MSCs.

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“…34 It was reported that small-sized MSCs undergo rapid self-renewal and have a greater potential for multipotent differentiation than larger cells. 35 The engraftment of MSCs demonstrates site-specific and multipotent differentiation, and they are present at sites of wound healing and tissue regeneration after transplantation. 36 We found that some of the grafted MSCs had differentiated into endothelial cells and formed capillaries.…”

Section: Discussionmentioning

confidence: 99%

Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair

Zhang¹,

Zhou²,

Zhou³

et al. 2017

IJN

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Prosthetic meshes used for hernioplasty are usually complicated with chronic pain due to avascular fibrotic scar or mesh shrinkage. In this study, we developed a tissue-engineered mesh (TEM) by seeding autologous bone marrow-derived mesenchymal stem cells onto nanosized fibers decellularized aorta (DA). DA was achieved by decellularizing the aorta sample sequentially with physical, mechanical, biological enzymatic digestion, and chemical detergent processes. The tertiary structure of DA was constituted with micro-, submicro-, and nanosized fibers, and the original strength of fresh aorta was retained. Inguinal hernia rabbit models were treated with TEMs or acellular meshes (AMs). After implantation, TEM-treated rabbit models showed no hernia recurrence, whereas AM-treated animals displayed bulges in inguinal area. At harvest, TEMs were thicker, have less adhesion, and have stronger mechanical strength compared to AMs ( P <0.05). Moreover, TEM showed better cell infiltration, tissue regeneration, and neovascularization ( P <0.05). Therefore, these cell-seeded DAs with nanosized fibers have potential for use in inguinal hernioplasty.

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Establishing Criteria for Human Mesenchymal Stem Cell Potency

Cited by 175 publications

References 58 publications

A Molecular Classification of Human Mesenchymal Stromal Cells

A Molecular Classification of Human Mesenchymal Stromal Cells

Epigenetic regulation of specific transcription factors in osteogenicdifferentiation of mesenchymal stem cells

Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair

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