Abstract:Semisynthetic Ras proteins are efficient probes for cell‐biology experiments. With a Bodipy FL fluorophore introduced at an appropriate site on the Ras peptide by solid‐phase synthesis, the resulting Ras chimera is processed by the cellular machinery and the intracellular localization of the protein can then be visualized by means of confocal laser fluorescence microscopy at relatively low concentrations. The absence of a large N‐terminal protein tag overcomes possible interferences in the interaction with cel… Show more
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