Establishing an essential amino acid profile for maintenance in poultry using deletion method

Dorigam, J. C. P.; Sakomura, Nilva Kazue; Lima, Michele Bernardino de; Sarcinelli, M. F.; Suzuki, Rafael Massami

doi:10.1111/jpn.12403

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…However, there is still an overall average effect when comparing young animal values of 185 versus 158 for adult animals, showing the influence of growth. However, this difference may have the influence of the studied amino acid lysine that has important effect on broiler growth (Dorigam et al, 2016), and future studies can be developed with other amino acids to confirm our hypothesis.…”

Section: Discussionmentioning

confidence: 75%

Alternative procedure for determining lysine maintenance requirement in poultry

Dorigam¹,

Silva²,

Sakomura³

et al. 2020

Revista Brasileira De Zootecnia

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Non-ruminants Full-length research article Alternative procedure for determining lysine maintenance requirement in poultry ABSTRACT-The objective with this study was to determine the lysine maintenance requirements (LMR) of male and female broilers as animal models. A total of 252 birds were used for nitrogen balance trials during three periods (I: 6-21, II: 22-37, and III: 38-53 days). Six lysine levels were used (2.76, 5.88, 8.99, 12.1, 15.2, and 18.3 g kg −1) with six replications. A control group also included, totalizing seven treatments for males and females for each assay. The experimental period was 15 days. The response variables included nitrogen intake (NI) and excretion (NEX), and their difference was assumed to be deposited as nitrogen. An exponential regression between NEX and lysine intake (LI) was fitted, and LMR was estimated when LI = 0. The daily values for LMR were 9.29, 33.4, and 40.2 mg BW 0.67 kg −1 for males and 9.36, 30.0, and 39.4 mg BW 0.67 kg −1 for females. The final value for both sexes were 10.1 (period I), 31.5 (period II), and 39.8 mg BW 0.67 kg −1 (period III). Expressed as body protein weight at maturity (BPm), the LMR were 172 and 148 (period I), 216 and 207 (period II), and 189 and 180 mg BPm 0.73 kg −1 (period III) for males and females, respectively. The results provided ranges of LMR values recommended in previous studies, validating this procedure. The procedure to estimate the requirements presented here provides new insights into the model of amino acid requirement estimations.

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Section: Discussionmentioning

confidence: 75%

Alternative procedure for determining lysine maintenance requirement in poultry

Dorigam¹,

Silva²,

Sakomura³

et al. 2020

Revista Brasileira De Zootecnia

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“…One of the things that alters the abundance of these metabolites in feces may be inefficient nutrient absorption caused by coccidiosis. , With the impairment of intestinal absorption, the nutrient could be excreted without absorption by the chicken. For example, methionine and phenylalanine are essential amino acids for poultry and are used as supplementary dietery. − In our model, methionine was weighted toward infected chickens. On the contrary, the abundance of phenylacetaldehyde, one of the metabolites of phenylalanine, is lower in infected chickens and weighted negatively in the Lasso model.…”

Section: Results and Discussionmentioning

confidence: 99%

On-Site Diagnosis of Poultry Coccidiosis by a Miniature Mass Spectrometer and Machine Learning

Wang

Chung

Dutkiewicz

et al. 2021

ACS Agric. Sci. Technol.

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Coccidiosis is a prevalent parasitic disease in poultry that costs the modern poultry industry more than US$3 billion worldwide every year for the resulting prevention, treatment, and related production loss. Currently, the diagnosis of coccidiosis relies on detecting the oocysts of Eimeria, a genus that causes coccidiosis, in feces by a veterinarian or polymerase chain reaction. However, both methods must be performed in a laboratory and could observe the oocysts of Eimeria only 4 days after infection. Herein, we present an on-site, early coccidiosis diagnosis platform by differentiating the fecal metabolic profile acquired using a miniature mass spectrometer between healthy and Eimeria tenella-infected chickens using a machine learning model. Our model achieved an accuracy of 94%, a sensitivity of 88%, and a specificity of 100%. It is noteworthy that our model can diagnose E. tenella infection 2 days earlier than current diagnostic methods. To make the mass spectrometry-based diagnosis platform usable in the field and feasible, a cart carrying all of the equipment and a user-friendly data processing graphical user interface was designed. On average, our diagnositc approach takes ∼9 min to obtain the results for each sample. This platform, miniature mass spectrometrybased metabolomics (M3S), provides a solution for early detection of parasite infection, from which an accurate, easy-to-determine on-site coccidiosis diagnosis can be realized.

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“…They are acceptable sources of proteins, energy, vitamins, fat, and minerals ( Doriana et al, 2020 ). In poultry, the provision of essential amino acids ( EAAs ) is a key factor to grow rapidly in a brief period ( Dorigam et al, 2016 ). Having higher concentrations of EAAs, earthworms are a natural source of protein for poultry ( Sun et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%