Abstract:Preliminary functional characterization of a gene within a short time period is only possible through transient gene expression assays. Transient gene expression assays are simple, cost effective and easy to perform as compared to generation of stable transformants. Here we report an Agro-infiltration based transient gene expression assay, optimized in Nicotiana tabacum using β-glucuronidase (GUS) intron gene as an indicator to evaluate various factors effecting its expression. The factors evaluated include ba… Show more
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