“…Such assays are performed wherever the presence of α-amylase needs to be determined, for example, in the human serum, urine, various physiologically active substances, and various food products, among others [ 23 ]. The most frequently used for alpha-Amylase assay are BpNPG7 (non-reducing-end blocked p-nitrophenyl malto-heptaoside) [ 24 , 25 , 26 , 27 , 28 , 29 ] and ethylidene-4-NP-G7 (4-nitrophenyl-4,6-ethylidene-alpha-D-maltoheptaoside) [ 30 , 31 ] which are also parts of commertial kits. Beta-Amylase assay using PNPβ-G3 (p-nitrophenyl-β-D-maltotrioside) [ 27 , 32 ] and BpNPG7 [ 26 ] was reported as well.…”