Establishing a rabbit model of perianal fistulizing Crohn’s disease

Lu, Shuangshuang; Liu, Wenjia; Niu, Qiuya; Huo, Chun-Yan; Cheng, Yuqing; Wang, En-Jing; Li, Rongnan; Feng, Feng; Cheng, Yi‐Ming; Liu, Rong; Huang, Jin

doi:10.3748/wjg.v28.i15.1536

Cited by 3 publications

(1 citation statement)

References 32 publications

(33 reference statements)

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“…Although first developed for use in rats [25], this model has become widely used for inducing colitis in zebrafish [57][58][59], mice [60,61], rabbits [62,63], pigs [64][65][66], and dogs [67][68][69]. TNBS is a hapten administered as an enema in combination with ethanol (30-50%) to break the mucosal barrier and allow TNBS penetration into the bowel wall [55].…”

Section: Plos Onementioning

confidence: 99%

Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis

Ocampo,

Castro,

Pájaro

et al. 2024

PLoS ONE

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Phytotherapy is an attractive strategy to treat inflammatory bowel disease (IBD) that could be especially useful in developing countries. We previously demonstrated the intestinal anti-inflammatory effect of the total ethereal extract from the Physalis peruviana (Cape gooseberry) calyces in TNBS-induced colitis. This work investigates the therapeutic potential of Peruviose A and B, two sucrose esters that constitute the major metabolites of its calyces. The effect of the Peruvioses A and B mixture on TNBS-induced colitis was studied after 3 (preventive) and 15-days (therapy set-up) of colitis induction in rats. Colonic inflammation was assessed by measuring macroscopic/histologic damage, MPO activity, and biochemical changes. Additionally, LPS-stimulated RAW 264.7 macrophages were treated with test compounds to determine the effect on cytokine imbalance in these cells. Peruvioses mixture ameliorated TNBS-induced colitis in acute (preventive) or established (therapeutic) settings. Although 3-day treatment with compounds did not produce a potent effect, it was sufficient to significantly reduce the extent/severity of tissue damage and the microscopic disturbances. Beneficial effects in the therapy set-up were substantially higher and involved the inhibition of pro-inflammatory enzymes (iNOS, COX-2), cytokines (TNF-α, IL-1β, and IL-6), as well as epithelial regeneration with restoration of goblet cells numbers and expression of MUC-2 and TFF-3. Consistently, LPS-induced RAW 264.7 cells produced less NO, PGE2, TNF-α, IL-6, and MCP-1. These effects might be related to the inhibition of the NF-κB signaling pathway. Our results suggest that sucrose esters from P. peruviana calyces, non-edible waste from fruit production, might be useful as an alternative IBD treatment.

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Section: Plos Onementioning

confidence: 99%

Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis

Ocampo,

Castro,

Pájaro

et al. 2024

PLoS ONE

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Pathogenesis of Perianal Fistulising Crohn’s Disease: Current Knowledge, Gaps in Understanding, and Future Research Directions

Cao

Colonna

Deepak

2023

Journal of Crohn's and Colitis

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Fistulizing perianal involvement is a common presentation of Crohn’s disease. Perianal Crohn’s disease dramatically impairs patients’ social and sexual life and its management remains a critical challenge in field of inflammatory bowel disease. No medical therapy is specifically designed for the treatment of perianal Crohn’s disease, largely due to the poorly understood pathophysiology. Limited evidence from pre-clinical and clinical studies suggest that the initiation, progression, and maintenance of Crohn’s disease-associated perianal fistulas involves complex interactions between host, microbial, and environmental factors. This review intends to discuss the possible roles of genetic predisposition, mucosal immunity, and gut microbiome in perianal Crohn’s disease, and considers how epithelial-to-mesenchymal transition may contribute to the pathogenesis. It also summarizes recent advances on the development of animal models and new therapies for perianal Crohn’s disease. In the end, we discussed future directions in basic, translational, and clinical research that may dramatically improve our understanding of perianal Crohn’s disease and open avenues for novel therapeutic strategies with a multidisciplinary approach. Overall, this review aims to identify current gaps in perianal fistulizing Crohn’s disease and explore future research priorities for this debilitating condition.

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Does Combined Medical and Surgical Treatment Improve Perianal Fistula Outcomes in Patients With Crohn’s Disease? A Systematic Review and Meta-Analysis

Fung,

Farbod,

Kankouni

et al. 2024

Journal of Crohn's and Colitis

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BACKGROUND The optimal treatment of perianal fistulizing Crohn’s disease (PFCD) is unknown. We performed a systematic review with meta-analysis to compare combined surgical intervention and anti-TNF therapy (combined therapy) vs. either therapy alone. METHODS MEDLINE, EMBASE, and Cochrane databases were searched systematically through December 2023. Surgical intervention was defined as an exam under anesthesia ± setons. We calculated weighted risk ratios (RR) with 95% confidence intervals (CI) for our co-primary outcomes: fistula response and healing, defined clinically as a reduction in fistula drainage or number of draining fistulas and fistula closure respectively. RESULTS Thirteen studies were analysed: 515 patients treated with combined therapy, 330 patients with surgical intervention and 406 patients with anti-TNF therapy with follow-up between 10 weeks and 3 years. Fistula response (RR 1.10; 95% CI, 0.93-1.30, p=0.28) and healing (RR 1.06; 95% CI, 0.86-1.31, p=0.58) was not significantly different when comparing combined therapy with anti-TNF therapy alone. In contrast, combined therapy was associated with significantly higher rates of fistula response (1.25; 95% CI, 1.10-1.41, p<0.001) and healing (RR 1.17; 95% CI, 1.00-1.36, p=0.05) compared with surgical intervention alone. Our results remained stable when limiting to studies that assessed outcomes within 1 year and studies where <10% of patients underwent fistula closure procedures. CONCLUSION Combined surgery and anti-TNF therapy was not associated with improved PFCD outcomes compared with anti-TNF therapy alone. Due to an inability to control for confounding and small study sizes, future, controlled trials are warranted to confirm these findings.

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Establishing a rabbit model of perianal fistulizing Crohn’s disease

Cited by 3 publications

References 32 publications

Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis

Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis

Pathogenesis of Perianal Fistulising Crohn’s Disease: Current Knowledge, Gaps in Understanding, and Future Research Directions

Does Combined Medical and Surgical Treatment Improve Perianal Fistula Outcomes in Patients With Crohn’s Disease? A Systematic Review and Meta-Analysis

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